Improved methodology for the affinity isolation of human protein complexes expressed at near endogenous levelsDomanski, M., Molloy, K., Jiang, H., Chait, B. T., Rout, M. P., Jensen, T. H. & LaCava, J., May-2012, In : Biotechniques. 0, 0, p. 1-6 6 p.
Research output: Contribution to journal › Article › Academic › peer-review
An efficient and reliable procedure for the capture of affinity-tagged proteins and associated complexes from human cell lines is reported. Through multiple optimizations, high yield and low background affinity-purifications are achieved from modest quantities of human cells expressing endogenous-level tagged proteins. Isolations of triple-FLAG and GFP-tagged fusion proteins involved in RNA metabolism are presented.
|Number of pages||6|
|Publication status||Published - May-2012|