Improved methodology for the affinity isolation of human protein complexes expressed at near endogenous levels

Domanski, M., Molloy, K., Jiang, H., Chait, B. T., Rout, M. P., Jensen, T. H. & LaCava, J., May-2012, In : Biotechniques. 0, 0, p. 1-6 6 p.

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  • Laboratory of Cellular and Structural Biology, The Rockefeller University, New York

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  • Michal Domanski
  • Kelly Molloy
  • Hua Jiang
  • Brian T Chait
  • Michael P Rout
  • Torben Heick Jensen
  • John LaCava

An efficient and reliable procedure for the capture of affinity-tagged proteins and associated complexes from human cell lines is reported. Through multiple optimizations, high yield and low background affinity-purifications are achieved from modest quantities of human cells expressing endogenous-level tagged proteins. Isolations of triple-FLAG and GFP-tagged fusion proteins involved in RNA metabolism are presented.

Original languageEnglish
Pages (from-to)1-6
Number of pages6
Issue number0
Publication statusPublished - May-2012
Externally publishedYes

ID: 117143353