Publication

Improved methodology for the affinity isolation of human protein complexes expressed at near endogenous levels

Domanski, M., Molloy, K., Jiang, H., Chait, B. T., Rout, M. P., Jensen, T. H. & LaCava, J., May-2012, In : Biotechniques. 0, 0, p. 1-6 6 p.

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  • Laboratory of Cellular and Structural Biology, The Rockefeller University, New York

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DOI

  • Michal Domanski
  • Kelly Molloy
  • Hua Jiang
  • Brian T Chait
  • Michael P Rout
  • Torben Heick Jensen
  • John LaCava

An efficient and reliable procedure for the capture of affinity-tagged proteins and associated complexes from human cell lines is reported. Through multiple optimizations, high yield and low background affinity-purifications are achieved from modest quantities of human cells expressing endogenous-level tagged proteins. Isolations of triple-FLAG and GFP-tagged fusion proteins involved in RNA metabolism are presented.

Original languageEnglish
Pages (from-to)1-6
Number of pages6
JournalBiotechniques
Volume0
Issue number0
Publication statusPublished - May-2012
Externally publishedYes

ID: 117143353