Publication

Impaired biosynthesis of the non-bilayer lipids phosphatidylethanolamine or cardiolipin does not affect peroxisome biogenesis and proliferation in Saccharomyces cerevisiae

Kawalek, A., Jagadeesan, C. & van der Klei, I. J., 11-Nov-2016, In : Biochemical and Biophysical Research Communications. 480, 2, p. 228-233 6 p.

Research output: Contribution to journalArticleAcademicpeer-review

APA

Kawalek, A., Jagadeesan, C., & van der Klei, I. J. (2016). Impaired biosynthesis of the non-bilayer lipids phosphatidylethanolamine or cardiolipin does not affect peroxisome biogenesis and proliferation in Saccharomyces cerevisiae. Biochemical and Biophysical Research Communications, 480(2), 228-233. https://doi.org/10.1016/j.bbrc.2016.10.033

Author

Kawalek, Adam ; Jagadeesan, Chandhuru ; van der Klei, Ida J. / Impaired biosynthesis of the non-bilayer lipids phosphatidylethanolamine or cardiolipin does not affect peroxisome biogenesis and proliferation in Saccharomyces cerevisiae. In: Biochemical and Biophysical Research Communications. 2016 ; Vol. 480, No. 2. pp. 228-233.

Harvard

Kawalek, A, Jagadeesan, C & van der Klei, IJ 2016, 'Impaired biosynthesis of the non-bilayer lipids phosphatidylethanolamine or cardiolipin does not affect peroxisome biogenesis and proliferation in Saccharomyces cerevisiae', Biochemical and Biophysical Research Communications, vol. 480, no. 2, pp. 228-233. https://doi.org/10.1016/j.bbrc.2016.10.033

Standard

Impaired biosynthesis of the non-bilayer lipids phosphatidylethanolamine or cardiolipin does not affect peroxisome biogenesis and proliferation in Saccharomyces cerevisiae. / Kawalek, Adam; Jagadeesan, Chandhuru; van der Klei, Ida J.

In: Biochemical and Biophysical Research Communications, Vol. 480, No. 2, 11.11.2016, p. 228-233.

Research output: Contribution to journalArticleAcademicpeer-review

Vancouver

Kawalek A, Jagadeesan C, van der Klei IJ. Impaired biosynthesis of the non-bilayer lipids phosphatidylethanolamine or cardiolipin does not affect peroxisome biogenesis and proliferation in Saccharomyces cerevisiae. Biochemical and Biophysical Research Communications. 2016 Nov 11;480(2):228-233. https://doi.org/10.1016/j.bbrc.2016.10.033


BibTeX

@article{f16e9ddfb2e4441f91dcf709bbbfd70f,
title = "Impaired biosynthesis of the non-bilayer lipids phosphatidylethanolamine or cardiolipin does not affect peroxisome biogenesis and proliferation in Saccharomyces cerevisiae",
abstract = "The non-bilayer forming lipids cardiolipin (CL) and phosphatidylethanolamine (PE) modulate membrane curvature, facilitate membrane fusion and affect the stability and function of membrane proteins. Yeast peroxisomal membranes contain significant amounts of CL and PE.We analysed the effect of CL deficiency and PE depletion on peroxisome biogenesis and proliferation in Saccharomyces cerevisiae. Our data indicate that deletion of CRD1, which encodes cardiolipin synthase, does not affect peroxisome biogenesis or abundance, both at peroxisome repressing (glucose) or inducing (oleate) growth conditions. Analysis of strains deficient in one of the three PE biosynthesis pathways (psdl, psd2 or the triple deletion strain ekil ckil dpll) revealed that in all three strains peroxisome numbers were reduced upon growth of cells on oleic acid, whereas the psdl strain also showed a reduction in peroxisome abundance upon growth on glucose. Because PE is an intermediate of the phosphatidylcholine (PC) biosynthesis pathway, PE depletion affects PC formation. PC however can be synthesized by an alternative pathway when choline is supplemented to the growth medium. Because the addition of choline resulted in suppression of the peroxisome phenotypes in phosphatidylserine decarboxylase mutant strains, we conclude that peroxisome biogenesis and proliferation are not crucially dependent on CL or PE. (C) 2016 Elsevier Inc. All rights reserved.",
keywords = "Peroxisome, Non-bilayer forming lipids, Cardiolipin, Phosphatidylethanolamine, PHOSPHATIDYLSERINE DECARBOXYLASE, MITOCHONDRIAL PHOSPHATIDYLETHANOLAMINE, HANSENULA-POLYMORPHA, YEAST, GENE, MEMBRANES, PATHWAYS, ACYLTRANSFERASE, IDENTIFICATION, ARABIDOPSIS",
author = "Adam Kawalek and Chandhuru Jagadeesan and {van der Klei}, {Ida J.}",
year = "2016",
month = "11",
day = "11",
doi = "10.1016/j.bbrc.2016.10.033",
language = "English",
volume = "480",
pages = "228--233",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "ACADEMIC PRESS INC ELSEVIER SCIENCE",
number = "2",

}

RIS

TY - JOUR

T1 - Impaired biosynthesis of the non-bilayer lipids phosphatidylethanolamine or cardiolipin does not affect peroxisome biogenesis and proliferation in Saccharomyces cerevisiae

AU - Kawalek, Adam

AU - Jagadeesan, Chandhuru

AU - van der Klei, Ida J.

PY - 2016/11/11

Y1 - 2016/11/11

N2 - The non-bilayer forming lipids cardiolipin (CL) and phosphatidylethanolamine (PE) modulate membrane curvature, facilitate membrane fusion and affect the stability and function of membrane proteins. Yeast peroxisomal membranes contain significant amounts of CL and PE.We analysed the effect of CL deficiency and PE depletion on peroxisome biogenesis and proliferation in Saccharomyces cerevisiae. Our data indicate that deletion of CRD1, which encodes cardiolipin synthase, does not affect peroxisome biogenesis or abundance, both at peroxisome repressing (glucose) or inducing (oleate) growth conditions. Analysis of strains deficient in one of the three PE biosynthesis pathways (psdl, psd2 or the triple deletion strain ekil ckil dpll) revealed that in all three strains peroxisome numbers were reduced upon growth of cells on oleic acid, whereas the psdl strain also showed a reduction in peroxisome abundance upon growth on glucose. Because PE is an intermediate of the phosphatidylcholine (PC) biosynthesis pathway, PE depletion affects PC formation. PC however can be synthesized by an alternative pathway when choline is supplemented to the growth medium. Because the addition of choline resulted in suppression of the peroxisome phenotypes in phosphatidylserine decarboxylase mutant strains, we conclude that peroxisome biogenesis and proliferation are not crucially dependent on CL or PE. (C) 2016 Elsevier Inc. All rights reserved.

AB - The non-bilayer forming lipids cardiolipin (CL) and phosphatidylethanolamine (PE) modulate membrane curvature, facilitate membrane fusion and affect the stability and function of membrane proteins. Yeast peroxisomal membranes contain significant amounts of CL and PE.We analysed the effect of CL deficiency and PE depletion on peroxisome biogenesis and proliferation in Saccharomyces cerevisiae. Our data indicate that deletion of CRD1, which encodes cardiolipin synthase, does not affect peroxisome biogenesis or abundance, both at peroxisome repressing (glucose) or inducing (oleate) growth conditions. Analysis of strains deficient in one of the three PE biosynthesis pathways (psdl, psd2 or the triple deletion strain ekil ckil dpll) revealed that in all three strains peroxisome numbers were reduced upon growth of cells on oleic acid, whereas the psdl strain also showed a reduction in peroxisome abundance upon growth on glucose. Because PE is an intermediate of the phosphatidylcholine (PC) biosynthesis pathway, PE depletion affects PC formation. PC however can be synthesized by an alternative pathway when choline is supplemented to the growth medium. Because the addition of choline resulted in suppression of the peroxisome phenotypes in phosphatidylserine decarboxylase mutant strains, we conclude that peroxisome biogenesis and proliferation are not crucially dependent on CL or PE. (C) 2016 Elsevier Inc. All rights reserved.

KW - Peroxisome

KW - Non-bilayer forming lipids

KW - Cardiolipin

KW - Phosphatidylethanolamine

KW - PHOSPHATIDYLSERINE DECARBOXYLASE

KW - MITOCHONDRIAL PHOSPHATIDYLETHANOLAMINE

KW - HANSENULA-POLYMORPHA

KW - YEAST

KW - GENE

KW - MEMBRANES

KW - PATHWAYS

KW - ACYLTRANSFERASE

KW - IDENTIFICATION

KW - ARABIDOPSIS

U2 - 10.1016/j.bbrc.2016.10.033

DO - 10.1016/j.bbrc.2016.10.033

M3 - Article

VL - 480

SP - 228

EP - 233

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 2

ER -

ID: 44601452