Publication

Impact of acute exposure to cigarette smoke on airway gene expression

Billatos, E., Faiz, A., Gesthalter, Y. O., LeClerc, A., Alekseyev, Y., Zhang, X., Liu, G., Ten Hacken, N. H. T., Heijink, I., Timens, W., Brandsma, C-A., Postma, D. S., van den Berge, M., Spira, A. & Lenburg, M. E., Sep-2018, In : Physiological Genomics. 50, 9, p. 705-713 9 p.

Research output: Contribution to journalArticleAcademicpeer-review

APA

Billatos, E., Faiz, A., Gesthalter, Y. O., LeClerc, A., Alekseyev, Y., Zhang, X., Liu, G., Ten Hacken, N. H. T., Heijink, I., Timens, W., Brandsma, C-A., Postma, D. S., van den Berge, M., Spira, A., & Lenburg, M. E. (2018). Impact of acute exposure to cigarette smoke on airway gene expression. Physiological Genomics, 50(9), 705-713. https://doi.org/10.1152/physiolgenomics.00092.2017

Author

Billatos, Ehab ; Faiz, Alen ; Gesthalter, Yaron O ; LeClerc, Ashley ; Alekseyev, Yuriy ; Zhang, Xiaohui ; Liu, Gang ; Ten Hacken, Nick H T ; Heijink, Irene ; Timens, Wim ; Brandsma, Corry-Anke ; Postma, Dirkje S ; van den Berge, Maarten ; Spira, Avrum ; Lenburg, Marc E. / Impact of acute exposure to cigarette smoke on airway gene expression. In: Physiological Genomics. 2018 ; Vol. 50, No. 9. pp. 705-713.

Harvard

Billatos, E, Faiz, A, Gesthalter, YO, LeClerc, A, Alekseyev, Y, Zhang, X, Liu, G, Ten Hacken, NHT, Heijink, I, Timens, W, Brandsma, C-A, Postma, DS, van den Berge, M, Spira, A & Lenburg, ME 2018, 'Impact of acute exposure to cigarette smoke on airway gene expression', Physiological Genomics, vol. 50, no. 9, pp. 705-713. https://doi.org/10.1152/physiolgenomics.00092.2017

Standard

Impact of acute exposure to cigarette smoke on airway gene expression. / Billatos, Ehab; Faiz, Alen; Gesthalter, Yaron O; LeClerc, Ashley; Alekseyev, Yuriy; Zhang, Xiaohui; Liu, Gang; Ten Hacken, Nick H T; Heijink, Irene; Timens, Wim; Brandsma, Corry-Anke; Postma, Dirkje S; van den Berge, Maarten; Spira, Avrum; Lenburg, Marc E.

In: Physiological Genomics, Vol. 50, No. 9, 09.2018, p. 705-713.

Research output: Contribution to journalArticleAcademicpeer-review

Vancouver

Billatos E, Faiz A, Gesthalter YO, LeClerc A, Alekseyev Y, Zhang X et al. Impact of acute exposure to cigarette smoke on airway gene expression. Physiological Genomics. 2018 Sep;50(9):705-713. https://doi.org/10.1152/physiolgenomics.00092.2017


BibTeX

@article{693d956267744913bdaef92ce65718c1,
title = "Impact of acute exposure to cigarette smoke on airway gene expression",
abstract = "Background: Understanding effects of acute smoke exposure (ASE) on airway epithelial gene expression and their relationship with the effects of chronic smoke exposure may provide biological insights into the development of smoking-related respiratory diseases. Methods: Bronchial airway epithelial cell brushings were collected from 63 individuals without recent cigarette smoke exposure and before and 24 h after smoking three cigarettes. RNA from these samples was profiled on Affymetrix Human Gene 1.0 ST microarrays. Results: We identified 91 genes differentially expressed 24 h after ASE (false discovery rate <0.25). ASE induced genes involved in xenobiotic metabolism, oxidative stress, and inflammation and repressed genes related to cilium morphogenesis and cell cycle. While many genes altered by ASE are altered similarly in chronic smokers, metallothionein genes are induced by ASE and suppressed in chronic smokers. Metallothioneins are also suppressed in current and former smokers with lung cancer relative to those without lung cancer. Conclusions: Acute exposure to as little as three cigarettes and chronic smoking induce largely concordant changes in airway epithelial gene expression. Differences in short-term and long-term effects of smoking on metallothionein expression and their relationship to lung cancer requires further study given these enzymes' role in the oxidative stress response.",
keywords = "airway, cigarettes, epithelial cells, gene expression, smoking, BRONCHIAL EPITHELIAL-CELLS, OBSTRUCTIVE PULMONARY-DISEASE, SET ENRICHMENT ANALYSIS, LUNG-CANCER, DIAGNOSTIC EVALUATION, TOBACCO-SMOKE, PROBE LEVEL, RELEASE, NEUTROPHIL",
author = "Ehab Billatos and Alen Faiz and Gesthalter, {Yaron O} and Ashley LeClerc and Yuriy Alekseyev and Xiaohui Zhang and Gang Liu and {Ten Hacken}, {Nick H T} and Irene Heijink and Wim Timens and Corry-Anke Brandsma and Postma, {Dirkje S} and {van den Berge}, Maarten and Avrum Spira and Lenburg, {Marc E}",
year = "2018",
month = sep,
doi = "10.1152/physiolgenomics.00092.2017",
language = "English",
volume = "50",
pages = "705--713",
journal = "Physiological Genomics",
issn = "1094-8341",
publisher = "AMER PHYSIOLOGICAL SOC",
number = "9",

}

RIS

TY - JOUR

T1 - Impact of acute exposure to cigarette smoke on airway gene expression

AU - Billatos, Ehab

AU - Faiz, Alen

AU - Gesthalter, Yaron O

AU - LeClerc, Ashley

AU - Alekseyev, Yuriy

AU - Zhang, Xiaohui

AU - Liu, Gang

AU - Ten Hacken, Nick H T

AU - Heijink, Irene

AU - Timens, Wim

AU - Brandsma, Corry-Anke

AU - Postma, Dirkje S

AU - van den Berge, Maarten

AU - Spira, Avrum

AU - Lenburg, Marc E

PY - 2018/9

Y1 - 2018/9

N2 - Background: Understanding effects of acute smoke exposure (ASE) on airway epithelial gene expression and their relationship with the effects of chronic smoke exposure may provide biological insights into the development of smoking-related respiratory diseases. Methods: Bronchial airway epithelial cell brushings were collected from 63 individuals without recent cigarette smoke exposure and before and 24 h after smoking three cigarettes. RNA from these samples was profiled on Affymetrix Human Gene 1.0 ST microarrays. Results: We identified 91 genes differentially expressed 24 h after ASE (false discovery rate <0.25). ASE induced genes involved in xenobiotic metabolism, oxidative stress, and inflammation and repressed genes related to cilium morphogenesis and cell cycle. While many genes altered by ASE are altered similarly in chronic smokers, metallothionein genes are induced by ASE and suppressed in chronic smokers. Metallothioneins are also suppressed in current and former smokers with lung cancer relative to those without lung cancer. Conclusions: Acute exposure to as little as three cigarettes and chronic smoking induce largely concordant changes in airway epithelial gene expression. Differences in short-term and long-term effects of smoking on metallothionein expression and their relationship to lung cancer requires further study given these enzymes' role in the oxidative stress response.

AB - Background: Understanding effects of acute smoke exposure (ASE) on airway epithelial gene expression and their relationship with the effects of chronic smoke exposure may provide biological insights into the development of smoking-related respiratory diseases. Methods: Bronchial airway epithelial cell brushings were collected from 63 individuals without recent cigarette smoke exposure and before and 24 h after smoking three cigarettes. RNA from these samples was profiled on Affymetrix Human Gene 1.0 ST microarrays. Results: We identified 91 genes differentially expressed 24 h after ASE (false discovery rate <0.25). ASE induced genes involved in xenobiotic metabolism, oxidative stress, and inflammation and repressed genes related to cilium morphogenesis and cell cycle. While many genes altered by ASE are altered similarly in chronic smokers, metallothionein genes are induced by ASE and suppressed in chronic smokers. Metallothioneins are also suppressed in current and former smokers with lung cancer relative to those without lung cancer. Conclusions: Acute exposure to as little as three cigarettes and chronic smoking induce largely concordant changes in airway epithelial gene expression. Differences in short-term and long-term effects of smoking on metallothionein expression and their relationship to lung cancer requires further study given these enzymes' role in the oxidative stress response.

KW - airway

KW - cigarettes

KW - epithelial cells

KW - gene expression

KW - smoking

KW - BRONCHIAL EPITHELIAL-CELLS

KW - OBSTRUCTIVE PULMONARY-DISEASE

KW - SET ENRICHMENT ANALYSIS

KW - LUNG-CANCER

KW - DIAGNOSTIC EVALUATION

KW - TOBACCO-SMOKE

KW - PROBE LEVEL

KW - RELEASE

KW - NEUTROPHIL

U2 - 10.1152/physiolgenomics.00092.2017

DO - 10.1152/physiolgenomics.00092.2017

M3 - Article

C2 - 29932825

VL - 50

SP - 705

EP - 713

JO - Physiological Genomics

JF - Physiological Genomics

SN - 1094-8341

IS - 9

ER -

ID: 62383873