Publication

Immunosensing with artificial antibodies in organic solvents or complex matrices

Schirhagl, R., Qian, J. & Dickert, F. L., 2012, In : Sensors and Actuators B: Chemical. 173, p. 585-590 6 p.

Research output: Contribution to journalArticleAcademicpeer-review

APA

Schirhagl, R., Qian, J., & Dickert, F. L. (2012). Immunosensing with artificial antibodies in organic solvents or complex matrices. Sensors and Actuators B: Chemical, 173, 585-590. https://doi.org/10.1016/j.snb.2012.07.036

Author

Schirhagl, Romana ; Qian, Jianjin ; Dickert, Franz L. / Immunosensing with artificial antibodies in organic solvents or complex matrices. In: Sensors and Actuators B: Chemical. 2012 ; Vol. 173. pp. 585-590.

Harvard

Schirhagl, R, Qian, J & Dickert, FL 2012, 'Immunosensing with artificial antibodies in organic solvents or complex matrices', Sensors and Actuators B: Chemical, vol. 173, pp. 585-590. https://doi.org/10.1016/j.snb.2012.07.036

Standard

Immunosensing with artificial antibodies in organic solvents or complex matrices. / Schirhagl, Romana; Qian, Jianjin; Dickert, Franz L.

In: Sensors and Actuators B: Chemical, Vol. 173, 2012, p. 585-590.

Research output: Contribution to journalArticleAcademicpeer-review

Vancouver

Schirhagl R, Qian J, Dickert FL. Immunosensing with artificial antibodies in organic solvents or complex matrices. Sensors and Actuators B: Chemical. 2012;173:585-590. https://doi.org/10.1016/j.snb.2012.07.036


BibTeX

@article{250b83b4a2a84e57ab64a2c1003b282a,
title = "Immunosensing with artificial antibodies in organic solvents or complex matrices",
abstract = "The detection of analytes in complex matrices without labour intensive sample preparation is an important goal in analytical chemistry. In this article we would like to address this issue by transferring the selectivity of natural antibody in a cheap, robust and reusable polymer, employing a double imprinting protocol. Antibodies with the desired selectivity were used as template to generate imprinted polymer particles. These antibodies were added to a prepolymer and particles were precipitated. After the antibodies were removed from the particles, cavities remained which reproduce size, shape and surface chemistry of the antibodies. In a second imprinting step the particles with cavities were pressed into a second polymer. After the second polymer has cured the particles can be removed leaving positive structures behind that react with the desired antigen. Such a sensitive coating was applied to the surface of a quartz crystal microbalance and incorporated into a microfluidic chip. An immunosensor for estradiol was fabricated having six times higher affinity to its antigen than to structurally related molecules. The measurements can also be performed after an extraction into an organic solvent which improves the detection limit greatly and would not be possible for natural antibodies. The feasibility of the method for complex matrices was shown by detecting viruses in plasma or allergenic PROTEIN in bread extract.",
author = "Romana Schirhagl and Jianjin Qian and Dickert, {Franz L}",
year = "2012",
doi = "10.1016/j.snb.2012.07.036",
language = "English",
volume = "173",
pages = "585--590",
journal = "Sensors and Actuators B: Chemical",
issn = "0925-4005",
publisher = "Elsevier Science",

}

RIS

TY - JOUR

T1 - Immunosensing with artificial antibodies in organic solvents or complex matrices

AU - Schirhagl, Romana

AU - Qian, Jianjin

AU - Dickert, Franz L

PY - 2012

Y1 - 2012

N2 - The detection of analytes in complex matrices without labour intensive sample preparation is an important goal in analytical chemistry. In this article we would like to address this issue by transferring the selectivity of natural antibody in a cheap, robust and reusable polymer, employing a double imprinting protocol. Antibodies with the desired selectivity were used as template to generate imprinted polymer particles. These antibodies were added to a prepolymer and particles were precipitated. After the antibodies were removed from the particles, cavities remained which reproduce size, shape and surface chemistry of the antibodies. In a second imprinting step the particles with cavities were pressed into a second polymer. After the second polymer has cured the particles can be removed leaving positive structures behind that react with the desired antigen. Such a sensitive coating was applied to the surface of a quartz crystal microbalance and incorporated into a microfluidic chip. An immunosensor for estradiol was fabricated having six times higher affinity to its antigen than to structurally related molecules. The measurements can also be performed after an extraction into an organic solvent which improves the detection limit greatly and would not be possible for natural antibodies. The feasibility of the method for complex matrices was shown by detecting viruses in plasma or allergenic PROTEIN in bread extract.

AB - The detection of analytes in complex matrices without labour intensive sample preparation is an important goal in analytical chemistry. In this article we would like to address this issue by transferring the selectivity of natural antibody in a cheap, robust and reusable polymer, employing a double imprinting protocol. Antibodies with the desired selectivity were used as template to generate imprinted polymer particles. These antibodies were added to a prepolymer and particles were precipitated. After the antibodies were removed from the particles, cavities remained which reproduce size, shape and surface chemistry of the antibodies. In a second imprinting step the particles with cavities were pressed into a second polymer. After the second polymer has cured the particles can be removed leaving positive structures behind that react with the desired antigen. Such a sensitive coating was applied to the surface of a quartz crystal microbalance and incorporated into a microfluidic chip. An immunosensor for estradiol was fabricated having six times higher affinity to its antigen than to structurally related molecules. The measurements can also be performed after an extraction into an organic solvent which improves the detection limit greatly and would not be possible for natural antibodies. The feasibility of the method for complex matrices was shown by detecting viruses in plasma or allergenic PROTEIN in bread extract.

U2 - 10.1016/j.snb.2012.07.036

DO - 10.1016/j.snb.2012.07.036

M3 - Article

VL - 173

SP - 585

EP - 590

JO - Sensors and Actuators B: Chemical

JF - Sensors and Actuators B: Chemical

SN - 0925-4005

ER -

ID: 14809853