IMMUNOHISTOLOGICAL CHARACTERIZATION OF PROXIMAL COLONIC LYMPHOID-TISSUE IN THE RATDEBOER, NK., KROESE, FGM., SHARP, JG. & PERRY, GA., Aug-1992, In : Anatomical record. 233, 4, p. 569-576 8 p.
Research output: Contribution to journal › Article › Academic › peer-review
Proximal colonic lymphoid tissue (PCLT) is a lymphoid structure located in the proximal colon of the mouse and the rat. In the present investigation we studied the immunomorphology and cytology of PCLT in the rat. We also studied sites of lymphocyte proliferation using the BrdU-anti BrdU technique.
Results demonstrated no evident phenotypical differences between the lymphocyte populations of PCLT and either jejunal or ileal Peyer's patches (PP). The majority of the lymphocytes within PCLT were B cells localized in follicles, which were separated from each other by interfollicular T cell areas. Germinal centers (GC), containing ED5+ follicular dendritic cells, are found within PCLT follicles. The T cell areas contained both MHC Class II+ interdigitating cells and high endothelial venules. Studies using BrdU-anti BrdU indicated that lymphocyte proliferation within PCLT takes place mainly in germinal centers.
Together the data show that the organization, lymphoid constituents, and sites of lymphocyte production are very similar in PCLT and PP. We therefore conclude that PCLT in the rat is not a Bursa equivalent, but more likely a PP with some special characteristics.
|Number of pages||8|
|Publication status||Published - Aug-1992|
- MONOCLONAL-ANTIBODIES, MACROPHAGE SUBPOPULATIONS, GERMINAL-CENTERS, LYMPHOCYTES-T, CELLS, DIFFERENTIATION, POPULATION, ENDOTOXIN, ANTIGENS, MOUSE