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Identification of quantitative trait loci regulating haematopoietic parameters in B6AKRF2 mice

van Os, R., Ausema, A., Noach, EJK., van Pelt, K., Dontje, BJH., Vellenga, E. & de Haan, G., Jan-2006, In : British Journal of Haematology. 132, 1, p. 80-90 11 p.

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  • Van Osetal 2006 BJH

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DOI

The haematopoietic system is a complex organised tissue with a hierarchical structure. Identification of organisational pathways within the haematopoietic system is relevant for a better understanding of haematopoiesis in health and disease. We have analysed numerous haematopoicticm parameters in two panels of a total of 157 genetically distinct B6AKRF2 mice, derived from an intercross between AKR and C57B1/6 mice, strains known to differ in various stem cell traits. The major objective of our study was to assess the extent to which various haematopoietic parameters, such as stem cell numbers, progenitor cell cycling, progenitor cell mobilisation and neutrophil numbers in blood and bone marrow are coregulated. The genotypes of these mice were used to search for genetic loci that regulate these parameters. We found significant quantitative trait loci (QTL) associated with the number of stem cells (CAFC-35) in the bone marrow and the number of neutrophils in the blood. However, most haematopoietic parameters appeared to be controlled by non-heritable (epigenetic) factors, or by multiple QTLs. Our study reveals striking differences in structure of the haematopoietic hierarchy between individual mice. Surprisingly, stem and progenitor cell pool size and proliferation rate, as well as peripheral blood cell counts are all independently regulated.

Original languageEnglish
Pages (from-to)80-90
Number of pages11
JournalBritish Journal of Haematology
Volume132
Issue number1
Publication statusPublished - Jan-2006

    Keywords

  • stem cells, genetic analysis, haematopoiesis, mobilisation, COLONY-STIMULATING FACTOR, PROGENITOR-CELL MOBILIZATION, STEM-CELLS, GENETIC-ANALYSIS, SELF-RENEWAL, LIFE-SPAN, IN-VIVO, MOUSE, GENERATION, FREQUENCY

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