Identification of epigenetic DNA modifications with a protein nanoporeWallace, E. VB., Stoddart, D., Heron, A., Mikhailova, E., Maglia, G., Donohoe, T. J. & Bayley, H., 2010, In : Chemical Communications. 46, 43, p. 8195-8197 3 p.
Research output: Contribution to journal › Article › Academic › peer-review
Two DNA bases, 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (hmC), marks of epigenetic modification, are recognized in immobilized DNA strands and distinguished from G, A, T and C by nanopore current recording. Therefore, if further aspects of nanopore sequencing can be addressed, the approach will provide a means to locate epigenetic modifications in unamplified genomic DNA.
|Number of pages||3|
|Publication status||Published - 2010|
- single-molecule, polynucleotide molecules, biological nanopore, human genome, methylation, 5-HYDROXYMETHYLCYTOSINE, 5-METHYLCYTOSINE, DISCRIMINATION, CONVERSION