Publication

Identification of epigenetic DNA modifications with a protein nanopore

Wallace, E. VB., Stoddart, D., Heron, A., Mikhailova, E., Maglia, G., Donohoe, T. J. & Bayley, H., 2010, In : Chemical Communications. 46, 43, p. 8195-8197 3 p.

Research output: Contribution to journalArticleAcademicpeer-review

  • Emma VB Wallace
  • David Stoddart
  • Andrew Heron
  • E Mikhailova
  • Giovanni Maglia
  • Timothy J Donohoe
  • Hagan Bayley
Two DNA bases, 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (hmC), marks of epigenetic modification, are recognized in immobilized DNA strands and distinguished from G, A, T and C by nanopore current recording. Therefore, if further aspects of nanopore sequencing can be addressed, the approach will provide a means to locate epigenetic modifications in unamplified genomic DNA.
Original languageEnglish
Pages (from-to)8195-8197
Number of pages3
JournalChemical Communications
Volume46
Issue number43
Publication statusPublished - 2010
Externally publishedYes

    Keywords

  • single-molecule, polynucleotide molecules, biological nanopore, human genome, methylation, 5-HYDROXYMETHYLCYTOSINE, 5-METHYLCYTOSINE, DISCRIMINATION, CONVERSION

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