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Identification of CDP-Archaeol Synthase, a Missing Link of Ether Lipid Biosynthesis in Archaea

Jain, S., Caforio, A., Fodran, P., Lolkema, J. S., Minnaard, A. J. & Driessen, A. J. M., 23-Oct-2014, In : Chemistry & Biology. 21, 10, p. 1392-1401 10 p.

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  • Identification of CDP-Archaeol Synthase, a Missing Link of Ether Lipid Biosynthesis in Archaea

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DOI

Archaeal membrane lipid composition is distinct from Bacteria and Eukarya, consisting of isoprenoid chains etherified to the glycerol carbons. Biosynthesis of these lipids is poorly understood. Here we identify and characterize the archaeal membrane protein CDP-archaeol synthase (CarS) that catalyzes the transfer of the nucleotide to its specific archaeal lipid substrate, leading to the formation of a CDP-activated precursor (CDP-archaeol) to which polar head groups are attached. The discovery of CarS enabled reconstitution of the entire archaeal lipid biosynthesis pathway in vitro, starting from simple isoprenoid building blocks and using a set of five purified enzymes. The cell free synthetic strategy for archaeal lipids we describe opens opportunity for studies of archaeal lipid biochemistry. Additionally, insights into archaeal lipid biosynthesis reported here allow addressing the evolutionary hypothesis of the lipid divide between Archaea and Bacteria.

Original languageEnglish
Pages (from-to)1392-1401
Number of pages10
JournalChemistry & Biology
Volume21
Issue number10
Publication statusPublished - 23-Oct-2014

    Keywords

  • GERANYLGERANYLGLYCERYL PHOSPHATE SYNTHASE, YEAST SACCHAROMYCES-CEREVISIAE, HYDROPATHY PROFILE ALIGNMENT, DIACYLGLYCEROL SYNTHASE, ESCHERICHIA-COLI, PHOSPHOLIPID-SYNTHESIS, DIPHOSPHATE SYNTHASE, SN-GLYCEROL-1-PHOSPHATE DEHYDROGENASE, METHANOBACTERIUM-THERMOAUTOTROPHICUM, THERMOACIDOPHILIC ARCHAEON

ID: 16171847