Identification of bacterial invasion in necrotizing enterocolitis specimens using fluorescent in situ hybridizationHeida, F. H., Harmsen, H. J. M., Timmer, A., Kooi, E. M. W., Bos, A. F. & Hulscher, J. B. F., Jan-2017, In : Journal of perinatology. 37, 1, p. 67-72 6 p.
Research output: Contribution to journal › Article › Academic › peer-review
OBJECTIVE: Investigation of bacterial invasion into the intestinal wall in necrotizing enterocolitis (NEC) specimens.
STUDY DESIGN: We compared 43 surgical NEC specimens with 43 age-matched controls. We used fluorescent in situ hybridization (FISH), a universal bacterial probe together with species-specific probes for Clostridium spp., Enterobacteriaceae, bacteroides and enterococci/lactobacilli. We used a FISH scoring system to reveal invasion of the intestinal wall, in which 1 represented no colonies and 4 invasion of the intestinal wall.
RESULTS: We observed invasion of the intestinal wall in 22/43 of the most affected NEC tissue samples as compared with 16/43 in the least affected NEC tissue samples (P=-0.03). A FISH score of 4 was reached in 7/43 control cases. Enterobacteriaceae dominated the NEC specimens. Clostridium spp. were detected occasionally in NEC samples.
CONCLUSION: Bacterial invasion of the intestinal wall is more present in most affected NEC tissue samples compared with least affected NEC tissue samples or controls. Enterobacteriaceae are prevalent in advanced NEC.
|Number of pages||6|
|Journal||Journal of perinatology|
|Publication status||Published - Jan-2017|
- TARGETED OLIGONUCLEOTIDE PROBES, ESCHERICHIA-COLI, INTESTINAL MICROBIOTA, PRETERM INFANTS, GUT MICROBIOTA, POPULATIONS, TISSUE, BIRTH, HOST