Publication

Identification of a TLR2 Inhibiting Wheat Hydrolysate

Kiewiet, M. B. G., Dekkers, R., van Gool, M. P., Ulfman, L. H., Groeneveld, A., Faas, M. M. & de Vos, P., Dec-2018, In : Molecular Nutrition & Food Research. 62, 23, 10 p., 1800716.

Research output: Contribution to journalArticleAcademicpeer-review

APA

Kiewiet, M. B. G., Dekkers, R., van Gool, M. P., Ulfman, L. H., Groeneveld, A., Faas, M. M., & de Vos, P. (2018). Identification of a TLR2 Inhibiting Wheat Hydrolysate. Molecular Nutrition & Food Research, 62(23), [1800716]. https://doi.org/10.1002/mnfr.201800716

Author

Kiewiet, Mensiena B. G. ; Dekkers, Renske ; van Gool, Martine P. ; Ulfman, Laurien H. ; Groeneveld, Andre ; Faas, Marijke M. ; de Vos, Paul. / Identification of a TLR2 Inhibiting Wheat Hydrolysate. In: Molecular Nutrition & Food Research. 2018 ; Vol. 62, No. 23.

Harvard

Kiewiet, MBG, Dekkers, R, van Gool, MP, Ulfman, LH, Groeneveld, A, Faas, MM & de Vos, P 2018, 'Identification of a TLR2 Inhibiting Wheat Hydrolysate', Molecular Nutrition & Food Research, vol. 62, no. 23, 1800716. https://doi.org/10.1002/mnfr.201800716

Standard

Identification of a TLR2 Inhibiting Wheat Hydrolysate. / Kiewiet, Mensiena B. G.; Dekkers, Renske; van Gool, Martine P.; Ulfman, Laurien H.; Groeneveld, Andre; Faas, Marijke M.; de Vos, Paul.

In: Molecular Nutrition & Food Research, Vol. 62, No. 23, 1800716, 12.2018.

Research output: Contribution to journalArticleAcademicpeer-review

Vancouver

Kiewiet MBG, Dekkers R, van Gool MP, Ulfman LH, Groeneveld A, Faas MM et al. Identification of a TLR2 Inhibiting Wheat Hydrolysate. Molecular Nutrition & Food Research. 2018 Dec;62(23). 1800716. https://doi.org/10.1002/mnfr.201800716


BibTeX

@article{b08d1d0fc3944ea89ab1781b04976df6,
title = "Identification of a TLR2 Inhibiting Wheat Hydrolysate",
abstract = "Scope Wheat hydrolysates are used in medical nutrition to provide undernourished patients a readily digestible protein source, for instance to recover from chemotherapy-induced intestinal mucosal inflammation. Since many hydrolysates of different sources can modulate the immune system, likely via Toll-like receptors (TLRs), it is hypothesized that also wheat hydrolysates might interact with TLR signaling, which could be a way to prevent intestinal inflammation and damage.Methods and results The capacity of three wheat hydrolysates to modulate immunity by interfering with TLR signaling is determined. All wheat hydrolysates have TLR modulating effects but only one has strong TLR2 inhibiting effects, attenuating both TLR2/1 and TLR2/6 signaling in a reporter cell system. This is likely induced by direct TLR2-ectodomain binding, as confirmed by ELISA. Furthermore, this TLR2 blocking hydrolysate reduces IL-6 production in human dendritic cells. Application of reversed-phase-ultra HPLC combined with MS reveals that the presence of peptide WQIPEQSR is associated with the observed TLR2 inhibiting capacity.Conclusion The study demonstrates TLR2-inhibiting capacities of a wheat hydrolysate. The findings provide a good start for further research to investigate whether this hydrolysate might contribute to the management of intestinal mucosal inflammation in cancer patients receiving chemotherapy.",
keywords = "bioactive peptides, inhibition, mucositis, toll-like receptor 2, wheat hydrolysates, DENDRITIC CELLS, IMMUNOMODULATING PROPERTIES, INTESTINAL MUCOSITIS, PROTEIN HYDROLYSATE, IN-VITRO, RECEPTOR, DOXORUBICIN, DIETARY, GLUTEN, INFLAMMATION",
author = "Kiewiet, {Mensiena B. G.} and Renske Dekkers and {van Gool}, {Martine P.} and Ulfman, {Laurien H.} and Andre Groeneveld and Faas, {Marijke M.} and {de Vos}, Paul",
note = "This article is protected by copyright. All rights reserved.",
year = "2018",
month = "12",
doi = "10.1002/mnfr.201800716",
language = "English",
volume = "62",
journal = "Molecular Nutrition & Food Research",
issn = "1613-4125",
publisher = "Wiley",
number = "23",

}

RIS

TY - JOUR

T1 - Identification of a TLR2 Inhibiting Wheat Hydrolysate

AU - Kiewiet, Mensiena B. G.

AU - Dekkers, Renske

AU - van Gool, Martine P.

AU - Ulfman, Laurien H.

AU - Groeneveld, Andre

AU - Faas, Marijke M.

AU - de Vos, Paul

N1 - This article is protected by copyright. All rights reserved.

PY - 2018/12

Y1 - 2018/12

N2 - Scope Wheat hydrolysates are used in medical nutrition to provide undernourished patients a readily digestible protein source, for instance to recover from chemotherapy-induced intestinal mucosal inflammation. Since many hydrolysates of different sources can modulate the immune system, likely via Toll-like receptors (TLRs), it is hypothesized that also wheat hydrolysates might interact with TLR signaling, which could be a way to prevent intestinal inflammation and damage.Methods and results The capacity of three wheat hydrolysates to modulate immunity by interfering with TLR signaling is determined. All wheat hydrolysates have TLR modulating effects but only one has strong TLR2 inhibiting effects, attenuating both TLR2/1 and TLR2/6 signaling in a reporter cell system. This is likely induced by direct TLR2-ectodomain binding, as confirmed by ELISA. Furthermore, this TLR2 blocking hydrolysate reduces IL-6 production in human dendritic cells. Application of reversed-phase-ultra HPLC combined with MS reveals that the presence of peptide WQIPEQSR is associated with the observed TLR2 inhibiting capacity.Conclusion The study demonstrates TLR2-inhibiting capacities of a wheat hydrolysate. The findings provide a good start for further research to investigate whether this hydrolysate might contribute to the management of intestinal mucosal inflammation in cancer patients receiving chemotherapy.

AB - Scope Wheat hydrolysates are used in medical nutrition to provide undernourished patients a readily digestible protein source, for instance to recover from chemotherapy-induced intestinal mucosal inflammation. Since many hydrolysates of different sources can modulate the immune system, likely via Toll-like receptors (TLRs), it is hypothesized that also wheat hydrolysates might interact with TLR signaling, which could be a way to prevent intestinal inflammation and damage.Methods and results The capacity of three wheat hydrolysates to modulate immunity by interfering with TLR signaling is determined. All wheat hydrolysates have TLR modulating effects but only one has strong TLR2 inhibiting effects, attenuating both TLR2/1 and TLR2/6 signaling in a reporter cell system. This is likely induced by direct TLR2-ectodomain binding, as confirmed by ELISA. Furthermore, this TLR2 blocking hydrolysate reduces IL-6 production in human dendritic cells. Application of reversed-phase-ultra HPLC combined with MS reveals that the presence of peptide WQIPEQSR is associated with the observed TLR2 inhibiting capacity.Conclusion The study demonstrates TLR2-inhibiting capacities of a wheat hydrolysate. The findings provide a good start for further research to investigate whether this hydrolysate might contribute to the management of intestinal mucosal inflammation in cancer patients receiving chemotherapy.

KW - bioactive peptides

KW - inhibition

KW - mucositis

KW - toll-like receptor 2

KW - wheat hydrolysates

KW - DENDRITIC CELLS

KW - IMMUNOMODULATING PROPERTIES

KW - INTESTINAL MUCOSITIS

KW - PROTEIN HYDROLYSATE

KW - IN-VITRO

KW - RECEPTOR

KW - DOXORUBICIN

KW - DIETARY

KW - GLUTEN

KW - INFLAMMATION

U2 - 10.1002/mnfr.201800716

DO - 10.1002/mnfr.201800716

M3 - Article

VL - 62

JO - Molecular Nutrition & Food Research

JF - Molecular Nutrition & Food Research

SN - 1613-4125

IS - 23

M1 - 1800716

ER -

ID: 66889784