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Identification of a non-competitive inhibitor of Plasmodium falciparum aspartate transcarbamoylase

Lunev, S., Bosch, S. S., Batista, F. A., Wang, C., Li, J., Linzke, M., Kruithof, P., Chamoun, G., Dömling, A. S. S., Wrenger, C. & Groves, M. R., 11-Mar-2018, In : Biochemical and Biophysical Research Communications. 497, 3, p. 835-842 8 p.

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  • Identification of a non-competitive inhibitor

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DOI

Aspartate transcarbamoylase catalyzes the second step of de-novo pyrimidine biosynthesis. As malarial parasites lack pyrimidine salvage machinery and rely on de-novo production for growth and proliferation, this pathway is a target for drug discovery. Previously, an apo crystal structure of aspartate transcarbamoylase from Plasmodium falciparum (PfATC) in its T-state has been reported. Here we present crystal structures of PfATC in the liganded R-state as well as in complex with the novel inhibitor, 2,3-napthalenediol, identified by high-throughput screening. Our data shows that 2,3-napthalediol binds in close proximity to the active site, implying an allosteric mechanism of inhibition. Furthermore, we report biophysical characterization of 2,3-napthalenediol. These data provide a promising starting point for structure based drug design targeting PfATC and malarial de-novo pyrimidine biosynthesis.

Original languageEnglish
Pages (from-to)835-842
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume497
Issue number3
Early online date21-Feb-2018
Publication statusPublished - 11-Mar-2018

    Keywords

  • Journal Article

ID: 55021029