Publication

Hypoxia-inducible factor-1 and neuroglobin expression

Haines, B., Demaria, M., Mao, X., Xie, L., Campisi, J., Jin, K. & Greenberg, D. A., 18-Apr-2012, In : Neuroscience Letters. 514, 2, p. 137-40 4 p.

Research output: Contribution to journalArticleAcademicpeer-review

  • Bryan Haines
  • Marco Demaria
  • Xiao Mao
  • Lin Xie
  • Judith Campisi
  • Kunlin Jin
  • David A Greenberg

Neuroglobin (Ngb) is a hypoxia-inducible protein with cytoprotective effects in animal models of stroke, Alzheimer's disease, and related disorders, but the molecular mechanisms involved in its induction are unknown. We tested the hypothesis that hypoxia-inducible factor-1 (HIF-1) regulates Ngb levels, using shRNA-mediated knockdown and lentiviral vector-mediated overexpression of the HIF-1α subunit, in cultured neural (HN33) cells. HIF-1α knockdown decreased and HIF-1α overexpression increased Ngb levels, consistent with a connection between HIF-1 and Ngb induction. These findings may have implications for understanding the hypoxia-response repertoire of neural cells and devising therapeutic strategies for neurologic disorders.

Original languageEnglish
Pages (from-to)137-40
Number of pages4
JournalNeuroscience Letters
Volume514
Issue number2
Publication statusPublished - 18-Apr-2012
Externally publishedYes

    Keywords

  • Animals, Cell Line, Cells, Cultured, Globins, Hippocampus, Hypoxia-Inducible Factor 1, alpha Subunit, Mice, Nerve Tissue Proteins, RNA, Small Interfering

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