Publication

Hyper-reactive PMNs in Fc gamma RIIa 131 H/H genotype periodontitis patients

Nicu, E. A., Van der Velden, U., Everts, V., Van Winkelhoff, A. J., Roos, D. & Loos, B. G., Nov-2007, In : Journal of Clinical Periodontology. 34, 11, p. 938-945 8 p.

Research output: Contribution to journalArticleAcademicpeer-review

APA

Nicu, E. A., Van der Velden, U., Everts, V., Van Winkelhoff, A. J., Roos, D., & Loos, B. G. (2007). Hyper-reactive PMNs in Fc gamma RIIa 131 H/H genotype periodontitis patients. Journal of Clinical Periodontology, 34(11), 938-945. https://doi.org/10.1111/j.1600-051X.2007.01136.x

Author

Nicu, E A ; Van der Velden, U ; Everts, V ; Van Winkelhoff, A J ; Roos, D ; Loos, B G. / Hyper-reactive PMNs in Fc gamma RIIa 131 H/H genotype periodontitis patients. In: Journal of Clinical Periodontology. 2007 ; Vol. 34, No. 11. pp. 938-945.

Harvard

Nicu, EA, Van der Velden, U, Everts, V, Van Winkelhoff, AJ, Roos, D & Loos, BG 2007, 'Hyper-reactive PMNs in Fc gamma RIIa 131 H/H genotype periodontitis patients', Journal of Clinical Periodontology, vol. 34, no. 11, pp. 938-945. https://doi.org/10.1111/j.1600-051X.2007.01136.x

Standard

Hyper-reactive PMNs in Fc gamma RIIa 131 H/H genotype periodontitis patients. / Nicu, E A; Van der Velden, U; Everts, V; Van Winkelhoff, A J; Roos, D; Loos, B G.

In: Journal of Clinical Periodontology, Vol. 34, No. 11, 11.2007, p. 938-945.

Research output: Contribution to journalArticleAcademicpeer-review

Vancouver

Nicu EA, Van der Velden U, Everts V, Van Winkelhoff AJ, Roos D, Loos BG. Hyper-reactive PMNs in Fc gamma RIIa 131 H/H genotype periodontitis patients. Journal of Clinical Periodontology. 2007 Nov;34(11):938-945. https://doi.org/10.1111/j.1600-051X.2007.01136.x


BibTeX

@article{0028ea9f54ba40059a9588084603b542,
title = "Hyper-reactive PMNs in Fc gamma RIIa 131 H/H genotype periodontitis patients",
abstract = "Background: Receptors for the Fc part of IgG (Fc gamma RIIa) on polymorphonuclear leukocytes (PMN) mediate phagocytosis and cell activation. Previous results show that one of the genetic variants of the Fc gamma RIIa, the 131 H/H, is associated with more periodontal breakdown than the R/R. This may be due to hyper-reactivity of the H/H-PMNs upon interaction with bacteria.Aim: To study whether the Fc gamma RIIa genotype modifies the PMN reactivity in periodontitis patients.Material and Methods: A cohort of 98 periodontitis patients was genotyped. From these, 10 H/H and 10 R/R consented to participate. PMNs were incubated with immune serum-opsonized Actinobacillus actinomycetemcomitans (A.a.). Phagocytosis, degranulation (CD63 and CD66b expression), respiratory burst and elastase release were assessed.Results: Patients of the H/H genotype showed more bone loss than those with the H/R or R/R genotype (p=0.038). H/H-PMNs phagocytosed more opsonized A.a. than did R/R-PMNs (p=0.019). The H/H-PMNs also expressed more CD63 and CD66b than did the R/R-PMNs (p=0.004 and 0.002, respectively) and released more elastase (p=0.001).Conclusions: The genotyping results confirm previous reports that more periodontal destruction occurs in the H/H genotype than in the H/R or R/R genotype. The functional studies indicate a hyper-reactivity of the H/H-PMN in response to bacteria, which may be one of several pathways leading to more periodontal breakdown.",
keywords = "Fc gamma RIIa, genotype, periodontitis, PMN, reactivity, GAMMA-RECEPTOR-IIA, PERIPHERAL NEUTROPHILS, ACTINOBACILLUS-ACTINOMYCETEMCOMITANS, ADULT PERIODONTITIS, RISK-FACTORS, POLYMORPHISMS, LIPOPOLYSACCHARIDE, ASSOCIATION, DESTRUCTION, MACROPHAGES",
author = "Nicu, {E A} and {Van der Velden}, U and V Everts and {Van Winkelhoff}, {A J} and D Roos and Loos, {B G}",
year = "2007",
month = "11",
doi = "10.1111/j.1600-051X.2007.01136.x",
language = "English",
volume = "34",
pages = "938--945",
journal = "Journal of Clinical Periodontology",
issn = "0303-6979",
publisher = "Wiley",
number = "11",

}

RIS

TY - JOUR

T1 - Hyper-reactive PMNs in Fc gamma RIIa 131 H/H genotype periodontitis patients

AU - Nicu, E A

AU - Van der Velden, U

AU - Everts, V

AU - Van Winkelhoff, A J

AU - Roos, D

AU - Loos, B G

PY - 2007/11

Y1 - 2007/11

N2 - Background: Receptors for the Fc part of IgG (Fc gamma RIIa) on polymorphonuclear leukocytes (PMN) mediate phagocytosis and cell activation. Previous results show that one of the genetic variants of the Fc gamma RIIa, the 131 H/H, is associated with more periodontal breakdown than the R/R. This may be due to hyper-reactivity of the H/H-PMNs upon interaction with bacteria.Aim: To study whether the Fc gamma RIIa genotype modifies the PMN reactivity in periodontitis patients.Material and Methods: A cohort of 98 periodontitis patients was genotyped. From these, 10 H/H and 10 R/R consented to participate. PMNs were incubated with immune serum-opsonized Actinobacillus actinomycetemcomitans (A.a.). Phagocytosis, degranulation (CD63 and CD66b expression), respiratory burst and elastase release were assessed.Results: Patients of the H/H genotype showed more bone loss than those with the H/R or R/R genotype (p=0.038). H/H-PMNs phagocytosed more opsonized A.a. than did R/R-PMNs (p=0.019). The H/H-PMNs also expressed more CD63 and CD66b than did the R/R-PMNs (p=0.004 and 0.002, respectively) and released more elastase (p=0.001).Conclusions: The genotyping results confirm previous reports that more periodontal destruction occurs in the H/H genotype than in the H/R or R/R genotype. The functional studies indicate a hyper-reactivity of the H/H-PMN in response to bacteria, which may be one of several pathways leading to more periodontal breakdown.

AB - Background: Receptors for the Fc part of IgG (Fc gamma RIIa) on polymorphonuclear leukocytes (PMN) mediate phagocytosis and cell activation. Previous results show that one of the genetic variants of the Fc gamma RIIa, the 131 H/H, is associated with more periodontal breakdown than the R/R. This may be due to hyper-reactivity of the H/H-PMNs upon interaction with bacteria.Aim: To study whether the Fc gamma RIIa genotype modifies the PMN reactivity in periodontitis patients.Material and Methods: A cohort of 98 periodontitis patients was genotyped. From these, 10 H/H and 10 R/R consented to participate. PMNs were incubated with immune serum-opsonized Actinobacillus actinomycetemcomitans (A.a.). Phagocytosis, degranulation (CD63 and CD66b expression), respiratory burst and elastase release were assessed.Results: Patients of the H/H genotype showed more bone loss than those with the H/R or R/R genotype (p=0.038). H/H-PMNs phagocytosed more opsonized A.a. than did R/R-PMNs (p=0.019). The H/H-PMNs also expressed more CD63 and CD66b than did the R/R-PMNs (p=0.004 and 0.002, respectively) and released more elastase (p=0.001).Conclusions: The genotyping results confirm previous reports that more periodontal destruction occurs in the H/H genotype than in the H/R or R/R genotype. The functional studies indicate a hyper-reactivity of the H/H-PMN in response to bacteria, which may be one of several pathways leading to more periodontal breakdown.

KW - Fc gamma RIIa

KW - genotype

KW - periodontitis

KW - PMN

KW - reactivity

KW - GAMMA-RECEPTOR-IIA

KW - PERIPHERAL NEUTROPHILS

KW - ACTINOBACILLUS-ACTINOMYCETEMCOMITANS

KW - ADULT PERIODONTITIS

KW - RISK-FACTORS

KW - POLYMORPHISMS

KW - LIPOPOLYSACCHARIDE

KW - ASSOCIATION

KW - DESTRUCTION

KW - MACROPHAGES

U2 - 10.1111/j.1600-051X.2007.01136.x

DO - 10.1111/j.1600-051X.2007.01136.x

M3 - Article

C2 - 17877745

VL - 34

SP - 938

EP - 945

JO - Journal of Clinical Periodontology

JF - Journal of Clinical Periodontology

SN - 0303-6979

IS - 11

ER -

ID: 13926595