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Hyperbranched PEI with Various Oligosaccharide Architectures: Synthesis, Characterization, ATP Complexation, and Cellular Uptake Properties

Appelhans, D., Komber, H., Quadir, M. A., Richter, S., Schwarz, S., van der Vlist, J., Aigner, A., Mueller, M., Loos, K., Seidel, J., Arndt, K-F., Haag, R., Voit, B., Müller, M. & Seidel, J., May-2009, In : Biomacromolecules. 10, 5, p. 1114-1124 11 p.

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DOI

  • Dietmar Appelhans
  • Hartmut Komber
  • Mohiuddin Abdul Quadir
  • Sven Richter
  • Simona Schwarz
  • Jereon van der Vlist
  • Achim Aigner
  • Martin Mueller
  • Katja Loos
  • Juergen Seidel
  • Karl-Friedrich Arndt
  • Rainer Haag
  • Brigitte Voit
  • Martin Müller
  • Jürgen Seidel

We present a rapid synthetic method for the development of hyperbranched PEIs decorated with different oligosaccharide architectures as carrier systems (CS) for drugs and bioactive molecules for in vitro and in vivo experiments. Reductive amination of hyperbranched PEI with readily available oligosaccharides results in sugar functionalized PEI cores with oligosaccharide shells of different densities. These core-shell architectures were characterized by NMR spectroscopy, elemental analysis, SLS, DLS, IR, and polyelectrolyte titration experiments. ATP complexation of theses polycations was examined by isothermal titration calorimetry to evaluate the binding energy and ATP/CS complexation ratios under physiological conditions. In vitro experiments showed an enhanced cellular uptake of ATP/CS complexes compared to those of the free ATP molecules. The results arise to initiate further noncovalent complexation studies of pharmacologically relevant molecules that may lead to the development of therapeutics based on this polymeric delivery platform.

Original languageEnglish
Pages (from-to)1114-1124
Number of pages11
JournalBiomacromolecules
Volume10
Issue number5
Publication statusPublished - May-2009

    Keywords

  • DENDRITIC MULTISHELL ARCHITECTURES, POLY(PROPYLENE IMINE) DENDRIMERS, GENE DELIVERY, IN-VIVO, FUNCTIONALIZED DENDRIMERS, POLY(ETHYLENE IMINE), BINDING-PROPERTIES, CONCANAVALIN-A, DRUG-DELIVERY, POLYETHYLENIMINE

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