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Hydrogen-Driven Cofactor Regeneration for Stereoselective Whole-Cell C=C Bond Reduction in Cupriavidus necator

Assil-Companioni, L., Schmidt, S., Heidinger, P., Schwab, H. & Kourist, R., 7-Jun-2019, In : Chemsuschem. 12, 11, p. 2361-2365 5 p.

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  • Hydrogen‐Driven Cofactor Regeneration for Stereoselective Whole‐Cell C=C Bond Reduction in Cupriavidus necator

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DOI

  • Leen Assil-Companioni
  • Sandy Schmidt
  • Petra Heidinger
  • Helmut Schwab
  • Robert Kourist

The coupling of recombinantly expressed oxidoreductases to endogenous hydrogenases for cofactor recycling permits the omission of organic cosubstrates as sacrificial electron donors in whole-cell biotransformations. This increases atom efficiency and simplifies the reaction. A recombinant ene-reductase was expressed in the hydrogen-oxidizing proteobacterium Cupriavidus necator H16. In hydrogen-driven biotransformations, whole cells catalyzed asymmetric C=C bond reduction of unsaturated cyclic ketones with stereoselectivities up to >99 % enantiomeric excess. The use of hydrogen as a substrate for growth and cofactor regeneration is particularly attractive because it represents a strategy for improving atom efficiency and reducing side product formation associated with the recycling of organic cofactors.

Original languageEnglish
Pages (from-to)2361-2365
Number of pages5
JournalChemsuschem
Volume12
Issue number11
Publication statusPublished - 7-Jun-2019
Externally publishedYes

    Keywords

  • asymmetric synthesis, biocatalysis, biotransformation, hydrogenation, reduction, BIOCATALYTIC HYDROGENATION, RALSTONIA, OXIDATION

ID: 131271435