Hydrogen-Driven Cofactor Regeneration for Stereoselective Whole-Cell C=C Bond Reduction in Cupriavidus necatorAssil-Companioni, L., Schmidt, S., Heidinger, P., Schwab, H. & Kourist, R., 7-Jun-2019, In : Chemsuschem. 12, 11, p. 2361-2365 5 p.
Research output: Contribution to journal › Article › Academic › peer-review
The coupling of recombinantly expressed oxidoreductases to endogenous hydrogenases for cofactor recycling permits the omission of organic cosubstrates as sacrificial electron donors in whole-cell biotransformations. This increases atom efficiency and simplifies the reaction. A recombinant ene-reductase was expressed in the hydrogen-oxidizing proteobacterium Cupriavidus necator H16. In hydrogen-driven biotransformations, whole cells catalyzed asymmetric C=C bond reduction of unsaturated cyclic ketones with stereoselectivities up to >99 % enantiomeric excess. The use of hydrogen as a substrate for growth and cofactor regeneration is particularly attractive because it represents a strategy for improving atom efficiency and reducing side product formation associated with the recycling of organic cofactors.
|Number of pages||5|
|Publication status||Published - 7-Jun-2019|
- asymmetric synthesis, biocatalysis, biotransformation, hydrogenation, reduction, BIOCATALYTIC HYDROGENATION, RALSTONIA, OXIDATION