Publication

Hydrogen sulfide stimulates activation of hepatic stellate cells through increased cellular bio-energetics

Damba, T., Zhang, M., Buist-Homan, M., van Goor, H., Faber, K. N. & Moshage, H., 1-Nov-2019, In : Nitric oxide-Biology and chemistry. 92, 1, p. 26-33 8 p.

Research output: Contribution to journalArticleAcademicpeer-review

APA

Damba, T., Zhang, M., Buist-Homan, M., van Goor, H., Faber, K. N., & Moshage, H. (2019). Hydrogen sulfide stimulates activation of hepatic stellate cells through increased cellular bio-energetics. Nitric oxide-Biology and chemistry, 92(1), 26-33. https://doi.org/10.1016/j.niox.2019.08.004

Author

Damba, Turtushikh ; Zhang, Mengfan ; Buist-Homan, Manon ; van Goor, Harry ; Faber, Klaas Nico ; Moshage, Han. / Hydrogen sulfide stimulates activation of hepatic stellate cells through increased cellular bio-energetics. In: Nitric oxide-Biology and chemistry. 2019 ; Vol. 92, No. 1. pp. 26-33.

Harvard

Damba, T, Zhang, M, Buist-Homan, M, van Goor, H, Faber, KN & Moshage, H 2019, 'Hydrogen sulfide stimulates activation of hepatic stellate cells through increased cellular bio-energetics', Nitric oxide-Biology and chemistry, vol. 92, no. 1, pp. 26-33. https://doi.org/10.1016/j.niox.2019.08.004

Standard

Hydrogen sulfide stimulates activation of hepatic stellate cells through increased cellular bio-energetics. / Damba, Turtushikh; Zhang, Mengfan; Buist-Homan, Manon; van Goor, Harry; Faber, Klaas Nico; Moshage, Han.

In: Nitric oxide-Biology and chemistry, Vol. 92, No. 1, 01.11.2019, p. 26-33.

Research output: Contribution to journalArticleAcademicpeer-review

Vancouver

Damba T, Zhang M, Buist-Homan M, van Goor H, Faber KN, Moshage H. Hydrogen sulfide stimulates activation of hepatic stellate cells through increased cellular bio-energetics. Nitric oxide-Biology and chemistry. 2019 Nov 1;92(1):26-33. https://doi.org/10.1016/j.niox.2019.08.004


BibTeX

@article{e3a0d85e5aa84ca3bfa5a6f40c99392d,
title = "Hydrogen sulfide stimulates activation of hepatic stellate cells through increased cellular bio-energetics",
abstract = "Hepatic fibrosis is caused by chronic inflammation and characterized as the excessive accumulation of extra cellular matrix (ECM) by activated hepatic stellate cells (HSCs). Gasotransmitters like NO and CO are known to modulate inflammation and fibrosis, however, little is known about the role of the gasotransmitter hydrogen sulfide (H2S) in liver fibrogenesis and stellate cell activation. Endogenous H2S is produced by the enzymes cystathionine beta-synthase (CBS), cystathionine gamma-lyase (CTH) and 3-mercaptopyruvate sulfur transferase (MPST) [1]. The aim of this study was to elucidate the role of endogenously produced and/or exogenously administered H2S on rat hepatic stellate cell activation and fibrogenesis. Primary rat HSCs were culture-activated for 7 days and treated with different H2S releasing donors (slow releasing donor GYY4137, fast releasing donor NaHS) or inhibitors of the H2S producing enzymes CTH and CBS (DL-PAG, AOAA). The main message of our study is that mRNA and protein expression level of H2S synthesizing enzymes are low in HSCs compared to hepatocytes and Kupffer cells. However, H2S promotes hepatic stellate cell activation. This conclusion is based on the fact that production of H2S and mRNA and protein expression of its producing enzyme CTH are increased during hepatic stellate cell activation. Furthermore, exogenous H2S increased HSC proliferation while inhibitors of endogenous H2S production reduce proliferation and fibrotic makers of HSCs. The effect of H2S on stellate cell activation correlated with increased cellular bioenergetics. Our results indicate that the H2S generation in hepatic stellate cells is a target for anti-fibrotic intervention and that systemic interventions with H2S should take into account cell-specific effects of H2S.",
keywords = "Hydrogen sulfide, H2S, Cystathionine gamma-lyase, CTH, CSE, Hepatic fibrosis, Hepatic stellate cells, HSCs, MITOCHONDRIAL BIOENERGETIC FUNCTION, OXIDATIVE STRESS, RAT, LIVER, GASOTRANSMITTERS, PROLIFERATION, INHIBITION, EXPRESSION, FIBROSIS",
author = "Turtushikh Damba and Mengfan Zhang and Manon Buist-Homan and {van Goor}, Harry and Faber, {Klaas Nico} and Han Moshage",
note = "Copyright {\circledC} 2019. Published by Elsevier Inc.",
year = "2019",
month = "11",
day = "1",
doi = "10.1016/j.niox.2019.08.004",
language = "English",
volume = "92",
pages = "26--33",
journal = "Nitric oxide-Biology and chemistry",
issn = "1089-8603",
publisher = "ACADEMIC PRESS INC ELSEVIER SCIENCE",
number = "1",

}

RIS

TY - JOUR

T1 - Hydrogen sulfide stimulates activation of hepatic stellate cells through increased cellular bio-energetics

AU - Damba, Turtushikh

AU - Zhang, Mengfan

AU - Buist-Homan, Manon

AU - van Goor, Harry

AU - Faber, Klaas Nico

AU - Moshage, Han

N1 - Copyright © 2019. Published by Elsevier Inc.

PY - 2019/11/1

Y1 - 2019/11/1

N2 - Hepatic fibrosis is caused by chronic inflammation and characterized as the excessive accumulation of extra cellular matrix (ECM) by activated hepatic stellate cells (HSCs). Gasotransmitters like NO and CO are known to modulate inflammation and fibrosis, however, little is known about the role of the gasotransmitter hydrogen sulfide (H2S) in liver fibrogenesis and stellate cell activation. Endogenous H2S is produced by the enzymes cystathionine beta-synthase (CBS), cystathionine gamma-lyase (CTH) and 3-mercaptopyruvate sulfur transferase (MPST) [1]. The aim of this study was to elucidate the role of endogenously produced and/or exogenously administered H2S on rat hepatic stellate cell activation and fibrogenesis. Primary rat HSCs were culture-activated for 7 days and treated with different H2S releasing donors (slow releasing donor GYY4137, fast releasing donor NaHS) or inhibitors of the H2S producing enzymes CTH and CBS (DL-PAG, AOAA). The main message of our study is that mRNA and protein expression level of H2S synthesizing enzymes are low in HSCs compared to hepatocytes and Kupffer cells. However, H2S promotes hepatic stellate cell activation. This conclusion is based on the fact that production of H2S and mRNA and protein expression of its producing enzyme CTH are increased during hepatic stellate cell activation. Furthermore, exogenous H2S increased HSC proliferation while inhibitors of endogenous H2S production reduce proliferation and fibrotic makers of HSCs. The effect of H2S on stellate cell activation correlated with increased cellular bioenergetics. Our results indicate that the H2S generation in hepatic stellate cells is a target for anti-fibrotic intervention and that systemic interventions with H2S should take into account cell-specific effects of H2S.

AB - Hepatic fibrosis is caused by chronic inflammation and characterized as the excessive accumulation of extra cellular matrix (ECM) by activated hepatic stellate cells (HSCs). Gasotransmitters like NO and CO are known to modulate inflammation and fibrosis, however, little is known about the role of the gasotransmitter hydrogen sulfide (H2S) in liver fibrogenesis and stellate cell activation. Endogenous H2S is produced by the enzymes cystathionine beta-synthase (CBS), cystathionine gamma-lyase (CTH) and 3-mercaptopyruvate sulfur transferase (MPST) [1]. The aim of this study was to elucidate the role of endogenously produced and/or exogenously administered H2S on rat hepatic stellate cell activation and fibrogenesis. Primary rat HSCs were culture-activated for 7 days and treated with different H2S releasing donors (slow releasing donor GYY4137, fast releasing donor NaHS) or inhibitors of the H2S producing enzymes CTH and CBS (DL-PAG, AOAA). The main message of our study is that mRNA and protein expression level of H2S synthesizing enzymes are low in HSCs compared to hepatocytes and Kupffer cells. However, H2S promotes hepatic stellate cell activation. This conclusion is based on the fact that production of H2S and mRNA and protein expression of its producing enzyme CTH are increased during hepatic stellate cell activation. Furthermore, exogenous H2S increased HSC proliferation while inhibitors of endogenous H2S production reduce proliferation and fibrotic makers of HSCs. The effect of H2S on stellate cell activation correlated with increased cellular bioenergetics. Our results indicate that the H2S generation in hepatic stellate cells is a target for anti-fibrotic intervention and that systemic interventions with H2S should take into account cell-specific effects of H2S.

KW - Hydrogen sulfide

KW - H2S

KW - Cystathionine gamma-lyase

KW - CTH

KW - CSE

KW - Hepatic fibrosis

KW - Hepatic stellate cells

KW - HSCs

KW - MITOCHONDRIAL BIOENERGETIC FUNCTION

KW - OXIDATIVE STRESS

KW - RAT

KW - LIVER

KW - GASOTRANSMITTERS

KW - PROLIFERATION

KW - INHIBITION

KW - EXPRESSION

KW - FIBROSIS

U2 - 10.1016/j.niox.2019.08.004

DO - 10.1016/j.niox.2019.08.004

M3 - Article

VL - 92

SP - 26

EP - 33

JO - Nitric oxide-Biology and chemistry

JF - Nitric oxide-Biology and chemistry

SN - 1089-8603

IS - 1

ER -

ID: 93760933