Publication

Human scFv SIgA expressed on Lactococcus lactis as a vector for the treatment of mucosal disease

Yuvaraj, S., Lahham, S., Marreddy, R. K. R., Dijkstra, G., Wolken, W. A. M., Lolkema, J. S., Helfrich, W., Johansen, F-E., Peppelenbosch, M. P., Bos, N. A. & Marredy, R. K. R., Aug-2008, In : Molecular Nutrition & Food Research. 52, 8, p. 913-920 8 p.

Research output: Contribution to journalArticleAcademicpeer-review

Copy link to clipboard

Documents

DOI

The gastrointestinal tract is a complex niche and the main port of entry of many pathogens that trigger a wide range of diseases like inflammatory bowel disease (IBD) and colon cancer. Antibodies are effective for treating such diseases, but a System capable of local delivery at the site of the pathology, thus avoiding systemic side effects, is not yet available. Here we report a novel recombinant scFvSIgA1 protein produced by Lactococcus lactis, anchored to the bacterial membrane, which retains its full immuno-recognizing potential. This scFv fragment employed was specific for a colon cancer epilope, epithelial glycoprotein protein-2 (EGP-2). Accordingly L. lactis expressing this chimeric protein was capable of binding cells expressing this epitope. Expression of specific antibodies on bacteria may allow local delivery of anticancer agents produced by Such bacteria ill Conjunction with the antibody and provides a new avenue in the quest for targeted drug delivery.

Original languageEnglish
Pages (from-to)913-920
Number of pages8
JournalMolecular Nutrition & Food Research
Volume52
Issue number8
Publication statusPublished - Aug-2008

    Keywords

  • Lactococcus lactis, local delivery system, pIgR, recombinant, ScFv SIgA, PHASE-I TRIAL, ANTIBODY THERAPY, CROHNS-DISEASE, MONOCLONAL-ANTIBODIES, COLORECTAL-CARCINOMA, GM-CSF, CANCER, CELLS, 17-1A, EPITHELIUM

Download statistics

No data available

ID: 4772442