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Human Salivary Gland Stem Cells Functionally Restore Radiation Damaged Salivary Glands

Pringle, S., Maimets, M., van der Zwaag, M., Stokman, M. A., van Gosliga, D., Zwart, E., Witjes, M. J. H., de Haan, G., van Os, R. & Coppes, R. P., Mar-2016, In : STEM CELLS. 34, 3, p. 640-652 13 p.

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  • Human Salivary Gland Stem Cells Functionally Restore Radiation Damaged Salivary Glands

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DOI

Adult stem cells are often touted as therapeutic agents in the regenerative medicine field, however data detailing both the engraftment and functional capabilities of solid tissue derived human adult epithelial stem cells is scarce. Here we show the isolation of adult human salivary gland (SG) stem/progenitor cells and demonstrate at the single cell level in vitro self-renewal and differentiation into multilineage organoids. We also show in vivo functionality, long-term engraftment, and functional restoration in a xenotransplantation model. Indeed, transplanted human salisphere-derived cells restored saliva production and greatly improved the regenerative potential of irradiated SGs. Further selection for c-Kit expression enriched for cells with enhanced regenerative potencies. Interestingly, interaction of transplanted cells with the recipient SG may also be involved in functional recovery. Thus, we show for the first time that salispheres cultured from human SGs contain stem/progenitor cells capable of self-renewal and differentiation and rescue of saliva production. Our study underpins the therapeutic promise of salisphere cell therapy for the treatment of xerostomia.

Original languageEnglish
Pages (from-to)640-652
Number of pages13
JournalSTEM CELLS
Volume34
Issue number3
Publication statusPublished - Mar-2016

    Keywords

  • Human salivary gland stem cells, Radiation induced xerostomia, Adult stem cells, Differentiation, Organoids, MOUSE SUBMANDIBULAR-GLAND, IN-VITRO, BRANCHING MORPHOGENESIS, STEM/PROGENITOR CELLS, NECK RADIOTHERAPY, PROGENITOR CELLS, DUCT LIGATION, REGENERATION, EXPRESSION, PROLIFERATION

ID: 31561881