Human Milk Oligosaccharides Differently Modulate Goblet Cells Under Homeostatic, Proinflammatory Conditions and ER StressCheng, L., Kong, C., Walvoort, M. T. C., Faas, M. M. & de Vos, P., 11-Dec-2019, In : Molecular Nutrition & Food Research. 11 p., 1900976.
Research output: Contribution to journal › Article › Academic › peer-review
SCOPE: Human milk oligosaccharides (hMOs) have beneficial effects on intestinal barrier function, but the mechanisms of action are not well-understood. Here we study the effects of hMOs on goblet cells, which indicate that some hMOs may enhance mucus barrier function through direct modulation of goblet cell function.
METHODS AND RESULTS: The modulatory effects of 2'-FL, 3-FL, LNT2, and GOS on the expression of goblet cell secretory related genes MUC2, TFF3, RETNLB, and the Golgi-sulfotransferase genes CHST5, and GAL3ST2 of LS174T were determined by real-time quantitative RT-PCR. 3-FL, LNT2, and GOS modulated LS174T gene expression profiles in a dose and time-dependent manner. In addition, the up-regulation of MUC2 was confirmed by immunofluorescence staining. Effects of 2'-FL, 3-FL, LNT2, and GOS on gene transcription of LS174T were also assessed during exposure to TNF-α, IL-13, or tunicamycin. During TNF-α challenge, 3-FL and LNT2 enhanced MUC2 and TFF3 gene expression. After IL-13 exposure, 2'-FL, 3-FL, and LNT2 all showed up-regulating effects on MUC2, 3-FL and LNT2 also enhanced TFF3 expression. LNT2 significantly reversed Tm-induced down-regulating of TFF3, RETNLB, and CHST5.
CONCLUSION: Our findings indicate that hMOs may enhance mucus barrier function through direct modulation of intestinal goblet cells. Effects were structural and stressor-dependent way. This article is protected by copyright. All rights reserved.
|Number of pages||11|
|Journal||Molecular Nutrition & Food Research|
|Early online date||4-Dec-2019|
|Publication status||Published - 11-Dec-2019|
- ER-stress, goblet cells, human milk oligosaccharides, inflammatory condition, mucus barrier, BREAST-MILK, MESSENGER-RNA, EXPRESSION, MUCUS, BIOSYNTHESIS, SECRETION, PATHWAYS