How to screen non-viral gene delivery systems in vitro?

van Gaal, E. V. B., van Eijk, R., Oosting, R. S., Kok, R. J., Hennink, W. E., Crommelin, D. J. A. & Mastrobattista, E., 25-Sep-2011, In : Journal of controlled release : official journal of the Controlled Release Society. 154, 3, p. 218-32 15 p.

Research output: Contribution to journalArticleAcademicpeer-review

  • Ethlinn V B van Gaal
  • Roel van Eijk
  • Ronald S Oosting
  • Robbert Jan Kok
  • Wim E Hennink
  • Daan J A Crommelin
  • Enrico Mastrobattista

Screening of new gene delivery candidates regarding transfection efficiency and toxicity is usually performed by reading out transgene expression levels relative to a reference formulation after in vitro transfection. However, over the years and among different laboratories, this screening has been performed in a variety of cell lines, using a variety of conditions and read-out systems, and by comparison to a variety of reference formulations. This makes a direct comparison of results difficult, if not impossible. Reaching a consensus would enable placing new results into context of previous findings and estimate the overall contribution to the improvement of non-viral gene delivery. In this paper we illustrate the sensitivity of transfection outcomes on testing conditions chosen, and propose a screening protocol with the aim of standardization within the field.

Original languageEnglish
Pages (from-to)218-32
Number of pages15
JournalJournal of controlled release : official journal of the Controlled Release Society
Issue number3
Publication statusPublished - 25-Sep-2011
Externally publishedYes


  • Animals, DNA, Genetic Vectors, Humans, Plasmids, Polyethyleneimine, Transfection

ID: 19628876