Publication

High-Throughput Screening of Lipidomic Adaptations in Cultured Cells

Jeucken, A. & Brouwers, J. F., Feb-2019, In : Biomolecules. 9, 2, 14 p., 42.

Research output: Contribution to journalArticleAcademicpeer-review

APA

Jeucken, A., & Brouwers, J. F. (2019). High-Throughput Screening of Lipidomic Adaptations in Cultured Cells. Biomolecules, 9(2), [42]. https://doi.org/10.3390/biom9020042

Author

Jeucken, Aike ; Brouwers, Jos F. / High-Throughput Screening of Lipidomic Adaptations in Cultured Cells. In: Biomolecules. 2019 ; Vol. 9, No. 2.

Harvard

Jeucken, A & Brouwers, JF 2019, 'High-Throughput Screening of Lipidomic Adaptations in Cultured Cells', Biomolecules, vol. 9, no. 2, 42. https://doi.org/10.3390/biom9020042

Standard

High-Throughput Screening of Lipidomic Adaptations in Cultured Cells. / Jeucken, Aike; Brouwers, Jos F.

In: Biomolecules, Vol. 9, No. 2, 42, 02.2019.

Research output: Contribution to journalArticleAcademicpeer-review

Vancouver

Jeucken A, Brouwers JF. High-Throughput Screening of Lipidomic Adaptations in Cultured Cells. Biomolecules. 2019 Feb;9(2). 42. https://doi.org/10.3390/biom9020042


BibTeX

@article{5d89957f26d84a5ab27dba897da23d88,
title = "High-Throughput Screening of Lipidomic Adaptations in Cultured Cells",
abstract = "High-throughput screening of biologically active substances in cell cultures remains challenging despite great progress in contemporary lipidomic techniques. These experiments generate large amounts of data that are translated into lipid fingerprints. The subsequent visualization of lipidomic changes is key to meaningful interpretation of experimental results. As a demonstration of a rapid and versatile pipeline for lipidomic analysis, we cultured HeLa cells in 96-well format for four days in the presence or absence of various inhibitors of lipid metabolic pathways. Visualization of the data by principle component analysis revealed a high reproducibility of the method, as well as drug specific changes to the lipidome. Construction of heatmaps and networks revealed the similarities and differences between the effects of different drugs at the lipid species level. Clusters of related lipid species that might represent distinct membrane domains emerged after correlation analysis of the complete dataset. Taken together, we present a lipidomic platform for high-throughput lipidomic analysis of cultured cell lines.",
keywords = "lipidomics, autophagy, fatty acid synthase, high-throughput, phospholipids, lipid metabolism, FATTY-ACID SYNTHASE, MASS-SPECTROMETRIC ANALYSIS, THERAPEUTIC TARGET, SHOTGUN LIPIDOMICS, CHROMATOGRAPHY, IONIZATION, PALMITOYLTRANSFERASE, IDENTIFICATION, QUANTITATION, EXTRACTION",
author = "Aike Jeucken and Brouwers, {Jos F.}",
year = "2019",
month = "2",
doi = "10.3390/biom9020042",
language = "English",
volume = "9",
journal = "Biomolecules",
issn = "2218-273X",
publisher = "MDPI AG",
number = "2",

}

RIS

TY - JOUR

T1 - High-Throughput Screening of Lipidomic Adaptations in Cultured Cells

AU - Jeucken, Aike

AU - Brouwers, Jos F.

PY - 2019/2

Y1 - 2019/2

N2 - High-throughput screening of biologically active substances in cell cultures remains challenging despite great progress in contemporary lipidomic techniques. These experiments generate large amounts of data that are translated into lipid fingerprints. The subsequent visualization of lipidomic changes is key to meaningful interpretation of experimental results. As a demonstration of a rapid and versatile pipeline for lipidomic analysis, we cultured HeLa cells in 96-well format for four days in the presence or absence of various inhibitors of lipid metabolic pathways. Visualization of the data by principle component analysis revealed a high reproducibility of the method, as well as drug specific changes to the lipidome. Construction of heatmaps and networks revealed the similarities and differences between the effects of different drugs at the lipid species level. Clusters of related lipid species that might represent distinct membrane domains emerged after correlation analysis of the complete dataset. Taken together, we present a lipidomic platform for high-throughput lipidomic analysis of cultured cell lines.

AB - High-throughput screening of biologically active substances in cell cultures remains challenging despite great progress in contemporary lipidomic techniques. These experiments generate large amounts of data that are translated into lipid fingerprints. The subsequent visualization of lipidomic changes is key to meaningful interpretation of experimental results. As a demonstration of a rapid and versatile pipeline for lipidomic analysis, we cultured HeLa cells in 96-well format for four days in the presence or absence of various inhibitors of lipid metabolic pathways. Visualization of the data by principle component analysis revealed a high reproducibility of the method, as well as drug specific changes to the lipidome. Construction of heatmaps and networks revealed the similarities and differences between the effects of different drugs at the lipid species level. Clusters of related lipid species that might represent distinct membrane domains emerged after correlation analysis of the complete dataset. Taken together, we present a lipidomic platform for high-throughput lipidomic analysis of cultured cell lines.

KW - lipidomics

KW - autophagy

KW - fatty acid synthase

KW - high-throughput

KW - phospholipids

KW - lipid metabolism

KW - FATTY-ACID SYNTHASE

KW - MASS-SPECTROMETRIC ANALYSIS

KW - THERAPEUTIC TARGET

KW - SHOTGUN LIPIDOMICS

KW - CHROMATOGRAPHY

KW - IONIZATION

KW - PALMITOYLTRANSFERASE

KW - IDENTIFICATION

KW - QUANTITATION

KW - EXTRACTION

U2 - 10.3390/biom9020042

DO - 10.3390/biom9020042

M3 - Article

VL - 9

JO - Biomolecules

JF - Biomolecules

SN - 2218-273X

IS - 2

M1 - 42

ER -

ID: 103427449