Publication

High-Throughput Screening of Lipidomic Adaptations in Cultured Cells

Jeucken, A. & Brouwers, J. F., Feb-2019, In : Biomolecules. 9, 2, 14 p., 42.

Research output: Contribution to journalArticleAcademicpeer-review

High-throughput screening of biologically active substances in cell cultures remains challenging despite great progress in contemporary lipidomic techniques. These experiments generate large amounts of data that are translated into lipid fingerprints. The subsequent visualization of lipidomic changes is key to meaningful interpretation of experimental results. As a demonstration of a rapid and versatile pipeline for lipidomic analysis, we cultured HeLa cells in 96-well format for four days in the presence or absence of various inhibitors of lipid metabolic pathways. Visualization of the data by principle component analysis revealed a high reproducibility of the method, as well as drug specific changes to the lipidome. Construction of heatmaps and networks revealed the similarities and differences between the effects of different drugs at the lipid species level. Clusters of related lipid species that might represent distinct membrane domains emerged after correlation analysis of the complete dataset. Taken together, we present a lipidomic platform for high-throughput lipidomic analysis of cultured cell lines.

Original languageEnglish
Article number42
Number of pages14
JournalBiomolecules
Volume9
Issue number2
Publication statusPublished - Feb-2019

    Keywords

  • lipidomics, autophagy, fatty acid synthase, high-throughput, phospholipids, lipid metabolism, FATTY-ACID SYNTHASE, MASS-SPECTROMETRIC ANALYSIS, THERAPEUTIC TARGET, SHOTGUN LIPIDOMICS, CHROMATOGRAPHY, IONIZATION, PALMITOYLTRANSFERASE, IDENTIFICATION, QUANTITATION, EXTRACTION

Download statistics

No data available

ID: 103427449