Publication

Heterogeneity of young and aged murine hematopoietic stem cells revealed by quantitative clonal analysis using cellular barcoding

Verovskaya, E., Broekhuis, M. J. C., Zwart, E., Ritsema, M., van Os, R., de Haan, G. & Bystrykh, L. V., 25-Jul-2013, In : Blood. 122, 4, p. 523-532 10 p.

Research output: Contribution to journalArticleAcademicpeer-review

APA

Verovskaya, E., Broekhuis, M. J. C., Zwart, E., Ritsema, M., van Os, R., de Haan, G., & Bystrykh, L. V. (2013). Heterogeneity of young and aged murine hematopoietic stem cells revealed by quantitative clonal analysis using cellular barcoding. Blood, 122(4), 523-532. https://doi.org/10.1182/blood-2013-01-481135

Author

Verovskaya, Evgenia ; Broekhuis, Mathilde J. C. ; Zwart, Erik ; Ritsema, Martha ; van Os, Ronald ; de Haan, Gerald ; Bystrykh, Leonid V. / Heterogeneity of young and aged murine hematopoietic stem cells revealed by quantitative clonal analysis using cellular barcoding. In: Blood. 2013 ; Vol. 122, No. 4. pp. 523-532.

Harvard

Verovskaya, E, Broekhuis, MJC, Zwart, E, Ritsema, M, van Os, R, de Haan, G & Bystrykh, LV 2013, 'Heterogeneity of young and aged murine hematopoietic stem cells revealed by quantitative clonal analysis using cellular barcoding', Blood, vol. 122, no. 4, pp. 523-532. https://doi.org/10.1182/blood-2013-01-481135

Standard

Heterogeneity of young and aged murine hematopoietic stem cells revealed by quantitative clonal analysis using cellular barcoding. / Verovskaya, Evgenia; Broekhuis, Mathilde J. C.; Zwart, Erik; Ritsema, Martha; van Os, Ronald; de Haan, Gerald; Bystrykh, Leonid V.

In: Blood, Vol. 122, No. 4, 25.07.2013, p. 523-532.

Research output: Contribution to journalArticleAcademicpeer-review

Vancouver

Verovskaya E, Broekhuis MJC, Zwart E, Ritsema M, van Os R, de Haan G et al. Heterogeneity of young and aged murine hematopoietic stem cells revealed by quantitative clonal analysis using cellular barcoding. Blood. 2013 Jul 25;122(4):523-532. https://doi.org/10.1182/blood-2013-01-481135


BibTeX

@article{eff4d4913bba4fcd848fff20fc1d7d2e,
title = "Heterogeneity of young and aged murine hematopoietic stem cells revealed by quantitative clonal analysis using cellular barcoding",
abstract = "The number of hematopoietic stem cells (HSCs) that contributes to blood formation and the dynamics of their clonal contribution is a matter of ongoing discussion. Here, we use cellular barcoding combined with multiplex high-throughput sequencing to provide a quantitative and sensitive analysis of clonal behavior of hundreds of young and old HSCs. The majority of transplanted clones steadily contributes to hematopoiesis in the long-term, although clonal output in granulocytes, T cells, and B cells is substantially different. Contributions of individual clones to blood are dynamically changing; most of the clones either expand or decline with time. Finally, we demonstrate that the pool of old HSCs is composed of multiple small clones, whereas the young HSC pool is dominated by fewer, but larger, clones.",
keywords = "SELF-RENEWAL, IN-VIVO, MECHANISM, PROGRAMS, MICE",
author = "Evgenia Verovskaya and Broekhuis, {Mathilde J. C.} and Erik Zwart and Martha Ritsema and {van Os}, Ronald and {de Haan}, Gerald and Bystrykh, {Leonid V.}",
year = "2013",
month = "7",
day = "25",
doi = "10.1182/blood-2013-01-481135",
language = "English",
volume = "122",
pages = "523--532",
journal = "Blood",
issn = "0006-4971",
publisher = "AMER SOC HEMATOLOGY",
number = "4",

}

RIS

TY - JOUR

T1 - Heterogeneity of young and aged murine hematopoietic stem cells revealed by quantitative clonal analysis using cellular barcoding

AU - Verovskaya, Evgenia

AU - Broekhuis, Mathilde J. C.

AU - Zwart, Erik

AU - Ritsema, Martha

AU - van Os, Ronald

AU - de Haan, Gerald

AU - Bystrykh, Leonid V.

PY - 2013/7/25

Y1 - 2013/7/25

N2 - The number of hematopoietic stem cells (HSCs) that contributes to blood formation and the dynamics of their clonal contribution is a matter of ongoing discussion. Here, we use cellular barcoding combined with multiplex high-throughput sequencing to provide a quantitative and sensitive analysis of clonal behavior of hundreds of young and old HSCs. The majority of transplanted clones steadily contributes to hematopoiesis in the long-term, although clonal output in granulocytes, T cells, and B cells is substantially different. Contributions of individual clones to blood are dynamically changing; most of the clones either expand or decline with time. Finally, we demonstrate that the pool of old HSCs is composed of multiple small clones, whereas the young HSC pool is dominated by fewer, but larger, clones.

AB - The number of hematopoietic stem cells (HSCs) that contributes to blood formation and the dynamics of their clonal contribution is a matter of ongoing discussion. Here, we use cellular barcoding combined with multiplex high-throughput sequencing to provide a quantitative and sensitive analysis of clonal behavior of hundreds of young and old HSCs. The majority of transplanted clones steadily contributes to hematopoiesis in the long-term, although clonal output in granulocytes, T cells, and B cells is substantially different. Contributions of individual clones to blood are dynamically changing; most of the clones either expand or decline with time. Finally, we demonstrate that the pool of old HSCs is composed of multiple small clones, whereas the young HSC pool is dominated by fewer, but larger, clones.

KW - SELF-RENEWAL

KW - IN-VIVO

KW - MECHANISM

KW - PROGRAMS

KW - MICE

U2 - 10.1182/blood-2013-01-481135

DO - 10.1182/blood-2013-01-481135

M3 - Article

VL - 122

SP - 523

EP - 532

JO - Blood

JF - Blood

SN - 0006-4971

IS - 4

ER -

ID: 5929325