Publication

Heterogeneity of SOX9 and HNF1β in Pancreatic Ducts Is Dynamic

Rezanejad, H., Ouziel-Yahalom, L., Keyzer, C. A., Sullivan, B. A., Hollister-Lock, J., Li, W-C., Guo, L., Deng, S., Lei, J., Markmann, J. & Bonner-Weir, S., 13-Mar-2018, In : Stem Cell Reports. 10, 3, p. 725-738 14 p.

Research output: Contribution to journalArticleAcademicpeer-review

APA

Rezanejad, H., Ouziel-Yahalom, L., Keyzer, C. A., Sullivan, B. A., Hollister-Lock, J., Li, W-C., ... Bonner-Weir, S. (2018). Heterogeneity of SOX9 and HNF1β in Pancreatic Ducts Is Dynamic. Stem Cell Reports, 10(3), 725-738. https://doi.org/10.1016/j.stemcr.2018.01.028

Author

Rezanejad, Habib ; Ouziel-Yahalom, Limor ; Keyzer, Charlotte A ; Sullivan, Brooke A ; Hollister-Lock, Jennifer ; Li, Wan-Chun ; Guo, Lili ; Deng, Shaopeng ; Lei, Ji ; Markmann, James ; Bonner-Weir, Susan. / Heterogeneity of SOX9 and HNF1β in Pancreatic Ducts Is Dynamic. In: Stem Cell Reports. 2018 ; Vol. 10, No. 3. pp. 725-738.

Harvard

Rezanejad, H, Ouziel-Yahalom, L, Keyzer, CA, Sullivan, BA, Hollister-Lock, J, Li, W-C, Guo, L, Deng, S, Lei, J, Markmann, J & Bonner-Weir, S 2018, 'Heterogeneity of SOX9 and HNF1β in Pancreatic Ducts Is Dynamic', Stem Cell Reports, vol. 10, no. 3, pp. 725-738. https://doi.org/10.1016/j.stemcr.2018.01.028

Standard

Heterogeneity of SOX9 and HNF1β in Pancreatic Ducts Is Dynamic. / Rezanejad, Habib; Ouziel-Yahalom, Limor; Keyzer, Charlotte A; Sullivan, Brooke A; Hollister-Lock, Jennifer; Li, Wan-Chun; Guo, Lili; Deng, Shaopeng; Lei, Ji; Markmann, James; Bonner-Weir, Susan.

In: Stem Cell Reports, Vol. 10, No. 3, 13.03.2018, p. 725-738.

Research output: Contribution to journalArticleAcademicpeer-review

Vancouver

Rezanejad H, Ouziel-Yahalom L, Keyzer CA, Sullivan BA, Hollister-Lock J, Li W-C et al. Heterogeneity of SOX9 and HNF1β in Pancreatic Ducts Is Dynamic. Stem Cell Reports. 2018 Mar 13;10(3):725-738. https://doi.org/10.1016/j.stemcr.2018.01.028


BibTeX

@article{9e0c4e0115e54661bff121d1f2061f78,
title = "Heterogeneity of SOX9 and HNF1β in Pancreatic Ducts Is Dynamic",
abstract = "Pancreatic duct epithelial cells have been suggested as a source of progenitors for pancreatic growth and regeneration. However, genetic lineage-tracing experiments with pancreatic duct-specific Cre expression have given conflicting results. Using immunofluorescence and flow cytometry, we show heterogeneous expression of both HNF1β and SOX9 in adult human and murine ductal epithelium. Their expression was dynamic and diminished significantly after induced replication. Purified pancreatic duct cells formed organoid structures in 3D culture, and heterogeneity of expression of Hnf1β and Sox9 was maintained even after passaging. Using antibodies against a second cell surface molecule CD51 (human) or CD24 (mouse), we could isolate living subpopulations of duct cells enriched for high or low expression of HNF1β and SOX9. Only the CD24high (Hnfβhigh/Sox9high) subpopulation was able to form organoids.",
keywords = "Adult, Aged, Animals, CD24 Antigen/metabolism, Epithelial Cells/metabolism, Hepatocyte Nuclear Factor 1-beta/metabolism, Humans, Integrin alphaV/metabolism, Male, Mice, Mice, Inbred C57BL, Middle Aged, Organoids/metabolism, Pancreatic Ducts/metabolism, SOX9 Transcription Factor/metabolism",
author = "Habib Rezanejad and Limor Ouziel-Yahalom and Keyzer, {Charlotte A} and Sullivan, {Brooke A} and Jennifer Hollister-Lock and Wan-Chun Li and Lili Guo and Shaopeng Deng and Ji Lei and James Markmann and Susan Bonner-Weir",
note = "Copyright {\circledC} 2018 The Author(s). Published by Elsevier Inc. All rights reserved.",
year = "2018",
month = "3",
day = "13",
doi = "10.1016/j.stemcr.2018.01.028",
language = "English",
volume = "10",
pages = "725--738",
journal = "Stem Cell Reports",
issn = "2213-6711",
publisher = "CELL PRESS",
number = "3",

}

RIS

TY - JOUR

T1 - Heterogeneity of SOX9 and HNF1β in Pancreatic Ducts Is Dynamic

AU - Rezanejad, Habib

AU - Ouziel-Yahalom, Limor

AU - Keyzer, Charlotte A

AU - Sullivan, Brooke A

AU - Hollister-Lock, Jennifer

AU - Li, Wan-Chun

AU - Guo, Lili

AU - Deng, Shaopeng

AU - Lei, Ji

AU - Markmann, James

AU - Bonner-Weir, Susan

N1 - Copyright © 2018 The Author(s). Published by Elsevier Inc. All rights reserved.

PY - 2018/3/13

Y1 - 2018/3/13

N2 - Pancreatic duct epithelial cells have been suggested as a source of progenitors for pancreatic growth and regeneration. However, genetic lineage-tracing experiments with pancreatic duct-specific Cre expression have given conflicting results. Using immunofluorescence and flow cytometry, we show heterogeneous expression of both HNF1β and SOX9 in adult human and murine ductal epithelium. Their expression was dynamic and diminished significantly after induced replication. Purified pancreatic duct cells formed organoid structures in 3D culture, and heterogeneity of expression of Hnf1β and Sox9 was maintained even after passaging. Using antibodies against a second cell surface molecule CD51 (human) or CD24 (mouse), we could isolate living subpopulations of duct cells enriched for high or low expression of HNF1β and SOX9. Only the CD24high (Hnfβhigh/Sox9high) subpopulation was able to form organoids.

AB - Pancreatic duct epithelial cells have been suggested as a source of progenitors for pancreatic growth and regeneration. However, genetic lineage-tracing experiments with pancreatic duct-specific Cre expression have given conflicting results. Using immunofluorescence and flow cytometry, we show heterogeneous expression of both HNF1β and SOX9 in adult human and murine ductal epithelium. Their expression was dynamic and diminished significantly after induced replication. Purified pancreatic duct cells formed organoid structures in 3D culture, and heterogeneity of expression of Hnf1β and Sox9 was maintained even after passaging. Using antibodies against a second cell surface molecule CD51 (human) or CD24 (mouse), we could isolate living subpopulations of duct cells enriched for high or low expression of HNF1β and SOX9. Only the CD24high (Hnfβhigh/Sox9high) subpopulation was able to form organoids.

KW - Adult

KW - Aged

KW - Animals

KW - CD24 Antigen/metabolism

KW - Epithelial Cells/metabolism

KW - Hepatocyte Nuclear Factor 1-beta/metabolism

KW - Humans

KW - Integrin alphaV/metabolism

KW - Male

KW - Mice

KW - Mice, Inbred C57BL

KW - Middle Aged

KW - Organoids/metabolism

KW - Pancreatic Ducts/metabolism

KW - SOX9 Transcription Factor/metabolism

U2 - 10.1016/j.stemcr.2018.01.028

DO - 10.1016/j.stemcr.2018.01.028

M3 - Article

C2 - 29478894

VL - 10

SP - 725

EP - 738

JO - Stem Cell Reports

JF - Stem Cell Reports

SN - 2213-6711

IS - 3

ER -

ID: 78589687