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Hemorrhagic Shock-induced Endothelial Cell Activation in a Spontaneous Breathing and a Mechanical Ventilation Hemorrhagic Shock Model Is Induced by a Proinflammatory Response and Not by Hypoxia

van Meurs, M., Wulfert, F. M., Jongman, R. M., Schipper, M., Houwertjes, M. C., Vaneker, M., Scheffer, G. J., Teppema, L. J., Aarts, L. P. H. J., Heeringa, P., Zijlstra, J. G. & Molema, G., Sep-2011, In : Anesthesiology. 115, 3, p. 474-482 9 p.

Research output: Contribution to journalArticleAcademicpeer-review

Introduction: The interaction between neutrophils and activated endothelium is essential for the development of multiple organ dysfunction in patients with hemorrhagic shock (HS). Mechanical ventilation frequently is used in patients with HS. The authors sought to investigate the consequences of mechanical ventilation of mice subjected to HS on microvascular endothelial activation in the lung and kidney.

Methods: Anesthetized wild type C57BL/6 male mice were subjected to controlled hemorrhage; subgroups of mice were mechanically ventilated during the HS insult. To study the effect of acute hypoxia on the mice, the animals were housed in hypoxic cages. Gene expression levels was assessed by quantitative real-time polymerase chain reaction. Protein expression was assessed by immunohistochemistry and enzyme-linked immunosorbent assay.

Results: Ninety minutes after the shock induction, a vascular bed-specific, heterogeneous proinflammatory endothelial activation represented by E-selectin, vascular cell adhesion molecule 1, and intercellular adhesion molecule 1 expression was seen in kidney and lung. No differences in adhesion molecules between the spontaneously breathing and mechanically ventilated mice were found. Concentrations of the proinflammatory cytokines chemokine (C-X-C motif) ligand 1 (11.0-fold) and interleukin-6 (21.7-fold) were increased after 90 min of HS. Two hours of 6% oxygen did not induce the expression of E-selectin, vascular cell adhesion molecule 1, and intercellular adhesion molecule 1 in the kidneys and the lung.

Conclusions: Hemorrhagic shock leads to an early and reversible proinflammatory endothelial activation in kidney and lung. HS-induced endothelial activation is not changed by mechanical ventilation during the shock phase. Hypoxia alone does not lead to endothelial activation. The observed proinflammatory endothelial activation is mostly ischemiaor reperfusion-dependent and not related to hypoxia.

Original languageEnglish
Pages (from-to)474-482
Number of pages9
JournalAnesthesiology
Volume115
Issue number3
Publication statusPublished - Sep-2011

    Keywords

  • GENE-EXPRESSION, GROWTH-FACTOR, HEALTHY MICE, NITRIC-OXIDE, RESUSCITATION, LIPOPOLYSACCHARIDE, INTERMITTENT, MANAGEMENT, KIDNEY, INJURY

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