Publication

Hematopoietic stem cell expansion: challenges and opportunities

Walasek, M. A., van Os, R. & de Haan, G., 2012, HEMATOPOIETIC STEM CELLS VIII. Kanz, L., Fibbe, WE., Lengerke, C. & Dick, JE. (eds.). OXFORD: Blackwell Publishing, p. 138-150 13 p. (Annals of the New York Academy of Sciences; vol. 1266).

Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review

Attempts to improve hematopoietic reconstitution and engraftment potential of ex vivo-expanded hematopoietic stem and progenitor cells (HSPCs) have been largely unsuccessful due to the inability to generate sufficient stem cell numbers and to excessive differentiation of the starting cell population. Although hematopoietic stem cells (HSCs) will rapidly expand after in vivo transplantation, experience from in vitro studies indicates that control of HSPC self-renewal and differentiation in culture remains difficult. Protocols that are based on hematopoietic cytokines have failed to support reliable amplification of immature stem cells in culture, suggesting that additional factors are required. In recent years, several novel factors, including developmental factors and chemical compounds, have been reported to affect HSC self-renewal and improve ex vivo stem cell expansion protocols. Here, we highlight early expansion attempts and review recent development in the extrinsic control of HSPC fate in vitro.

Original languageEnglish
Title of host publicationHEMATOPOIETIC STEM CELLS VIII
EditorsL Kanz, WE Fibbe, C Lengerke, JE Dick
Place of PublicationOXFORD
PublisherBlackwell Publishing
Pages138-150
Number of pages13
ISBN (Print)978-1-57331-867-9
Publication statusPublished - 2012
Event8th International Conference and Workshop on Hematopoietic Stem Cells - , Germany
Duration: 22-Sep-201124-Sep-2011

Publication series

NameAnnals of the New York Academy of Sciences
PublisherBLACKWELL SCIENCE PUBL
Volume1266
ISSN (Print)0077-8923

Other

Other8th International Conference and Workshop on Hematopoietic Stem Cells
CountryGermany
Period22/09/201124/09/2011

Event

8th International Conference and Workshop on Hematopoietic Stem Cells

22/09/201124/09/2011

Germany

Event: Other

    Keywords

  • HSC expansion, cell-extrinsic factors, small molecules, EX-VIVO EXPANSION, COPPER CHELATOR TETRAETHYLENEPENTAMINE, UMBILICAL-CORD BLOOD, TRANS-RETINOIC ACID, MYELOID RECONSTITUTING ABILITY, SELF-RENEWAL CAPACITY, WNT GENE FAMILY, GROWTH-FACTOR-I, PROGENITOR CELLS, VALPROIC ACID

View graph of relations

ID: 5749927