Publication

Heat shock proteins are differentially expressed in brain and spinal cord: Implications for multiple sclerosis

Gorter, R. P., Nutma, E., Jahreiβ, M-C., de Jonge, J. C., Quinlan, R., van der Valk, P., van Noort, J. M., Baron, W. & Amor, S., Nov-2018, In : Clinical and Experimental Immunology. 194, 2, p. 137-152 16 p.

Research output: Contribution to journalArticleAcademicpeer-review

  • Rianne P Gorter
  • Erik Nutma
  • Marie-Christina Jahreiβ
  • Jenny C de Jonge
  • Roy Quinlan
  • Paul van der Valk
  • Johannes M van Noort
  • Wia Baron
  • Sandra Amor

AIMS: Multiple sclerosis (MS) is a chronic neurodegenerative disease characterised by demyelination, inflammation and neurodegeneration throughout the central nervous system. Although spinal cord pathology is an important factor contributing to disease progression, few studies have examined MS lesions in the spinal cord and how they differ from brain lesions. Here we have compared brain and spinal cord white and grey matter from MS and control tissues focussing on small heat shock proteins (HSPB) and HSP16.2.

METHODS: Western blotting was used to examine protein levels of HSPB1, HSPB5, HSPB6, HSPB8 and HSP16.2 in brain and spinal cord from MS and age-matched non-neurological controls. Immunohistochemistry was used to examine expression of the HSPs in MS spinal cord lesions and controls. Expression levels were quantified using ImageJ.

RESULTS: Western blotting revealed significantly higher levels of HSPB1, HSPB6 and HSPB8 in MS and control spinal cord compared to brain tissues. No differences in HSPB5 and HSP16.2 protein levels were observed although HSPB5 protein levels were higher in brain WM versus GM. In MS spinal cord lesions, increased HSPB1 and HSPB5 expression was observed in astrocytes, and increased neuronal expression of HSP16.2 was observed in normal appearing grey matter and type 1 grey matter lesions.

CONCLUSIONS: The high constitutive expression of several HSPBs in spinal cord and increased expression of HSPBs and HSP16.2 in MS illustrate differences between brain and spinal cord in health and upon demyelination. Regional differences in HSP expression may reflect differences in astrocyte cytoskeleton composition and influence inflammation, possibly affecting the effectiveness of pharmacological agents. This article is protected by copyright. All rights reserved.

Original languageEnglish
Pages (from-to)137-152
Number of pages16
JournalClinical and Experimental Immunology
Volume194
Issue number2
Publication statusPublished - Nov-2018

    Keywords

  • alpha-B crystallin, HSP16, 2, HSPBs, HSPB1, HSPB5, HSPB6, HSPB8, HSPB11, multiple sclerosis, pathology, spinal cord, ALPHA-B-CRYSTALLIN, INTRINSIC DIFFERENCES, GREY-MATTER, AXONAL LOSS, DEMYELINATION, INJURY, NEUROINFLAMMATION, PHOSPHORYLATION, MITOCHONDRIA, INFLAMMATION

Download statistics

No data available

ID: 63846332