Growing membranes in vitro by continuous phospholipid biosynthesis from free fatty acidsExterkate, M., Caforio, A., Stuart, M. C. A. & Driessen, A. J. M., Jan-2018, In : ACS Synthetic Biology. 7, 1, p. 153-165 13 p., acssynbio.7b00265.
Research output: Contribution to journal › Article › Academic › peer-review
One of the key aspects that defines a cell as a living entity is its ability to self-reproduce. In this process, membrane biogenesis is an essential element. Here, we developed an in vitro phospholipid biosynthesis pathway based on a cascade of eight enzymes, starting from simple fatty acid building blocks and glycerol 3-phosphate. The reconstituted system yields multiple phospholipid species that vary in acyl-chain and polar head group compositions. Due to the high fidelity and versatility, complete conversion of the fatty acid substrates into multiple phospholipid species is achieved simultaneously, leading to membrane expansion as a first step towards a synthetic minimal cell.
|Number of pages||13|
|Journal||ACS Synthetic Biology|
|Early online date||18-Sep-2017|
|Publication status||Published - Jan-2018|
- phospholipid biosynthesis, membrane biogenesis, enzyme cascade, reconstitution, GROWTH, CELLS, LIPID-PROTEIN INTERACTIONS, ESCHERICHIA-COLI, GIANT VESICLES, SN-GLYCEROL-3-PHOSPHATE ACYLTRANSFERASE, GLYCEROL 3-PHOSPHATE, SELF-REPRODUCTION, PURIFICATION, BACTERIA