Publication

Growing membranes in vitro by continuous phospholipid biosynthesis from free fatty acids

Exterkate, M., Caforio, A., Stuart, M. C. A. & Driessen, A. J. M., Jan-2018, In : ACS Synthetic Biology. 7, 1, p. 153-165 13 p., acssynbio.7b00265.

Research output: Contribution to journalArticleAcademicpeer-review

One of the key aspects that defines a cell as a living entity is its ability to self-reproduce. In this process, membrane biogenesis is an essential element. Here, we developed an in vitro phospholipid biosynthesis pathway based on a cascade of eight enzymes, starting from simple fatty acid building blocks and glycerol 3-phosphate. The reconstituted system yields multiple phospholipid species that vary in acyl-chain and polar head group compositions. Due to the high fidelity and versatility, complete conversion of the fatty acid substrates into multiple phospholipid species is achieved simultaneously, leading to membrane expansion as a first step towards a synthetic minimal cell.

Original languageEnglish
Article numberacssynbio.7b00265
Pages (from-to)153-165
Number of pages13
JournalACS Synthetic Biology
Volume7
Issue number1
Early online date18-Sep-2017
Publication statusPublished - Jan-2018

    Keywords

  • phospholipid biosynthesis, membrane biogenesis, enzyme cascade, reconstitution, GROWTH, CELLS, LIPID-PROTEIN INTERACTIONS, ESCHERICHIA-COLI, GIANT VESICLES, SN-GLYCEROL-3-PHOSPHATE ACYLTRANSFERASE, GLYCEROL 3-PHOSPHATE, SELF-REPRODUCTION, PURIFICATION, BACTERIA

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