Publication

Glucansucrases: Three-dimensional structures, reactions, mechanism, alpha-glucan analysis and their implications in biotechnology and food applications

Leemhuis, H., Pijning, T., Dobruchowska, J. M., van Leeuwen, S. S., Kralj, S., Dijkstra, B. W. & Dijkhuizen, L., 20-Jan-2013, In : Journal of Biotechnology. 163, 2, p. 250-272 23 p.

Research output: Contribution to journalArticleAcademicpeer-review

APA

Leemhuis, H., Pijning, T., Dobruchowska, J. M., van Leeuwen, S. S., Kralj, S., Dijkstra, B. W., & Dijkhuizen, L. (2013). Glucansucrases: Three-dimensional structures, reactions, mechanism, alpha-glucan analysis and their implications in biotechnology and food applications. Journal of Biotechnology, 163(2), 250-272. https://doi.org/10.1016/j.jbiotec.2012.06.037

Author

Leemhuis, Hans ; Pijning, Tjaard ; Dobruchowska, Justyna M. ; van Leeuwen, Sander S. ; Kralj, Slavko ; Dijkstra, Bauke W. ; Dijkhuizen, Lubbert. / Glucansucrases : Three-dimensional structures, reactions, mechanism, alpha-glucan analysis and their implications in biotechnology and food applications. In: Journal of Biotechnology. 2013 ; Vol. 163, No. 2. pp. 250-272.

Harvard

Leemhuis, H, Pijning, T, Dobruchowska, JM, van Leeuwen, SS, Kralj, S, Dijkstra, BW & Dijkhuizen, L 2013, 'Glucansucrases: Three-dimensional structures, reactions, mechanism, alpha-glucan analysis and their implications in biotechnology and food applications', Journal of Biotechnology, vol. 163, no. 2, pp. 250-272. https://doi.org/10.1016/j.jbiotec.2012.06.037

Standard

Glucansucrases : Three-dimensional structures, reactions, mechanism, alpha-glucan analysis and their implications in biotechnology and food applications. / Leemhuis, Hans; Pijning, Tjaard; Dobruchowska, Justyna M.; van Leeuwen, Sander S.; Kralj, Slavko; Dijkstra, Bauke W.; Dijkhuizen, Lubbert.

In: Journal of Biotechnology, Vol. 163, No. 2, 20.01.2013, p. 250-272.

Research output: Contribution to journalArticleAcademicpeer-review

Vancouver

Leemhuis H, Pijning T, Dobruchowska JM, van Leeuwen SS, Kralj S, Dijkstra BW et al. Glucansucrases: Three-dimensional structures, reactions, mechanism, alpha-glucan analysis and their implications in biotechnology and food applications. Journal of Biotechnology. 2013 Jan 20;163(2):250-272. https://doi.org/10.1016/j.jbiotec.2012.06.037


BibTeX

@article{1ac45646a56245b58d43e10adbaab774,
title = "Glucansucrases: Three-dimensional structures, reactions, mechanism, alpha-glucan analysis and their implications in biotechnology and food applications",
abstract = "Glucansucrases are extracellular enzymes that synthesize a wide variety of alpha-glucan polymers and oligosaccharides, such as dextran. These carbohydrates have found numerous applications in food and health industries, and can be used as pure compounds or even be produced in situ by generally regarded as safe (GRAS) lactic acid bacteria in food applications. Research in the recent years has resulted in big steps forward in the understanding and exploitation of the biocatalytic potential of glucansucrases. This paper provides an overview of glucansucrase enzymes, their recently elucidated crystal structures, their reaction and product specificity, and the structural analysis and applications of alpha-glucan polymers. Furthermore, we discuss key developments in the understanding of alpha-glucan polymer formation based on the recently elucidated three-dimensional structures of glucansucrase proteins. Finally we discuss the (potential) applications of alpha-glucans produced by lactic acid bacteria in food and health related industries. (C) 2012 Elsevier B.V. All rights reserved.",
keywords = "4,6-alpha-Glucanotransferase, Alternan, Alternansucrase, Dextran, Dextransucrase, Enzyme evolution, Mutansucrase, Reuteransucrase, Extracellular polysaccharide, Glucansucrase, Lactic acid bacteria, Mutan, Prebiotic, Protein engineering, Reuteran, Starch, MESENTEROIDES NRRL B-512F, LACTIC-ACID BACTERIA, STREPTOCOCCUS-MUTANS GLUCOSYLTRANSFERASES, LACTOBACILLUS-REUTERI 121, FIELD-FLOW FRACTIONATION, HUMAN GUT BACTERIA, ALTERNANSUCRASE ACCEPTOR REACTIONS, SIZE-EXCLUSION CHROMATOGRAPHY, LASER-LIGHT SCATTERING, IN-VITRO FERMENTATION",
author = "Hans Leemhuis and Tjaard Pijning and Dobruchowska, {Justyna M.} and {van Leeuwen}, {Sander S.} and Slavko Kralj and Dijkstra, {Bauke W.} and Lubbert Dijkhuizen",
year = "2013",
month = "1",
day = "20",
doi = "10.1016/j.jbiotec.2012.06.037",
language = "English",
volume = "163",
pages = "250--272",
journal = "Journal of Biotechnology",
issn = "0168-1656",
publisher = "Elsevier",
number = "2",

}

RIS

TY - JOUR

T1 - Glucansucrases

T2 - Three-dimensional structures, reactions, mechanism, alpha-glucan analysis and their implications in biotechnology and food applications

AU - Leemhuis, Hans

AU - Pijning, Tjaard

AU - Dobruchowska, Justyna M.

AU - van Leeuwen, Sander S.

AU - Kralj, Slavko

AU - Dijkstra, Bauke W.

AU - Dijkhuizen, Lubbert

PY - 2013/1/20

Y1 - 2013/1/20

N2 - Glucansucrases are extracellular enzymes that synthesize a wide variety of alpha-glucan polymers and oligosaccharides, such as dextran. These carbohydrates have found numerous applications in food and health industries, and can be used as pure compounds or even be produced in situ by generally regarded as safe (GRAS) lactic acid bacteria in food applications. Research in the recent years has resulted in big steps forward in the understanding and exploitation of the biocatalytic potential of glucansucrases. This paper provides an overview of glucansucrase enzymes, their recently elucidated crystal structures, their reaction and product specificity, and the structural analysis and applications of alpha-glucan polymers. Furthermore, we discuss key developments in the understanding of alpha-glucan polymer formation based on the recently elucidated three-dimensional structures of glucansucrase proteins. Finally we discuss the (potential) applications of alpha-glucans produced by lactic acid bacteria in food and health related industries. (C) 2012 Elsevier B.V. All rights reserved.

AB - Glucansucrases are extracellular enzymes that synthesize a wide variety of alpha-glucan polymers and oligosaccharides, such as dextran. These carbohydrates have found numerous applications in food and health industries, and can be used as pure compounds or even be produced in situ by generally regarded as safe (GRAS) lactic acid bacteria in food applications. Research in the recent years has resulted in big steps forward in the understanding and exploitation of the biocatalytic potential of glucansucrases. This paper provides an overview of glucansucrase enzymes, their recently elucidated crystal structures, their reaction and product specificity, and the structural analysis and applications of alpha-glucan polymers. Furthermore, we discuss key developments in the understanding of alpha-glucan polymer formation based on the recently elucidated three-dimensional structures of glucansucrase proteins. Finally we discuss the (potential) applications of alpha-glucans produced by lactic acid bacteria in food and health related industries. (C) 2012 Elsevier B.V. All rights reserved.

KW - 4,6-alpha-Glucanotransferase

KW - Alternan

KW - Alternansucrase

KW - Dextran

KW - Dextransucrase

KW - Enzyme evolution

KW - Mutansucrase

KW - Reuteransucrase

KW - Extracellular polysaccharide

KW - Glucansucrase

KW - Lactic acid bacteria

KW - Mutan

KW - Prebiotic

KW - Protein engineering

KW - Reuteran

KW - Starch

KW - MESENTEROIDES NRRL B-512F

KW - LACTIC-ACID BACTERIA

KW - STREPTOCOCCUS-MUTANS GLUCOSYLTRANSFERASES

KW - LACTOBACILLUS-REUTERI 121

KW - FIELD-FLOW FRACTIONATION

KW - HUMAN GUT BACTERIA

KW - ALTERNANSUCRASE ACCEPTOR REACTIONS

KW - SIZE-EXCLUSION CHROMATOGRAPHY

KW - LASER-LIGHT SCATTERING

KW - IN-VITRO FERMENTATION

U2 - 10.1016/j.jbiotec.2012.06.037

DO - 10.1016/j.jbiotec.2012.06.037

M3 - Article

VL - 163

SP - 250

EP - 272

JO - Journal of Biotechnology

JF - Journal of Biotechnology

SN - 0168-1656

IS - 2

ER -

ID: 5767077