Publication

Global mapping of the yeast genetic interaction network

Tong, AHY., Lesage, G., Bader, GD., Ding, HM., Xu, H., Xin, XF., Young, J., Berriz, GF., Brost, RL., Chang, M., Chen, YQ., Cheng, Y., Chua, G., Friesen, H., Goldberg, DS., Haynes, J., Humphries, C., He, G., Hussein, S., Ke, LZ., Krogan, N., Li, ZJ., Levinson, JN., Lu, H., Menard, P., Munyana, C., Parsons, AB., Ryan, O., Tonikian, R., Roberts, T., Sdicu, AM., Shapiro, J., Sheikh, B., Suter, B., Wong, SL., Zhang, LV., Zhu, HW., Burd, CG., Munro, S., Sander, C., Rine, J., Greenblatt, J., Peter, M., Bretscher, A., Bell, G., Roth, FP., Brown, GW., Andrews, B., Bussey, H. & Boone, C., 6-Feb-2004, In : Science. 303, 5659, p. 808-813 6 p.

Research output: Contribution to journalArticleAcademicpeer-review

  • AHY Tong
  • G Lesage
  • GD Bader
  • HM Ding
  • H Xu
  • XF Xin
  • J Young
  • GF Berriz
  • RL Brost
  • M Chang
  • YQ Chen
  • Yuqi Cheng
  • G Chua
  • H Friesen
  • DS Goldberg
  • J Haynes
  • C Humphries
  • G He
  • S Hussein
  • LZ Ke
  • N Krogan
  • ZJ Li
  • JN Levinson
  • H Lu
  • P Menard
  • C Munyana
  • AB Parsons
  • O Ryan
  • R Tonikian
  • T Roberts
  • AM Sdicu
  • J Shapiro
  • B Sheikh
  • B Suter
  • SL Wong
  • LV Zhang
  • HW Zhu
  • CG Burd
  • S Munro
  • C Sander
  • J Rine
  • J Greenblatt
  • M Peter
  • A Bretscher
  • G Bell
  • FP Roth
  • GW Brown
  • B Andrews
  • H Bussey
  • C Boone

A genetic interaction network containing similar to1000 genes and similar to4000 interactions was mapped by crossing mutations in 132 different query genes into a set of similar to4700 viable gene yeast deletion mutants and scoring the double mutant progeny for fitness defects. Network connectivity was predictive of function because interactions often occurred among functionally related genes, and similar patterns of interactions tended to identify components of the same pathway. The genetic network exhibited dense local neighborhoods; therefore, the position of a gene on a partially mapped network is predictive of other genetic interactions. Because digenic interactions are common in yeast, similar networks may underlie the complex genetics associated with inherited phenotypes in other organisms.

Original languageEnglish
Pages (from-to)808-813
Number of pages6
JournalScience
Volume303
Issue number5659
Publication statusPublished - 6-Feb-2004
Externally publishedYes

    Keywords

  • Amino Acid Sequence, Computational Biology, Cystic Fibrosis, Gene Deletion, Genes, Essential, Genes, Fungal, Genetic Diseases, Inborn, Genotype, Humans, Molecular Sequence Data, Multifactorial Inheritance, Mutation, Phenotype, Polymorphism, Genetic, Retinitis Pigmentosa, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins

ID: 14317165