Genomic approaches for identifying DNA damage response pathways in S-cerevisiae

Chang, M., Parsons, A. B., Sheikh, B. H., Boone, C. & Brown, G. W., 2006, Methods in Enzymology. Campbell, JL. & Modrich, P. (eds.). Academic Press, Vol. 409. p. 213-235 23 p. (Methods in Enzymology; vol. 409).

Research output: Chapter in Book/Report/Conference proceedingChapterAcademic

  • Michael Chang
  • Ainslie B. Parsons
  • Bilal H. Sheikh
  • Charles Boone
  • Grant W. Brown

DNA damage response pathways have been studied extensively in the budding yeast Saccharomyces cerevisiae, yet new genes with roles in the DNA damage response are still being identified. In this chapter we describe the use of functional genomic approaches in the identification of DNA damage response genes and pathways. These techniques take advantage of the S. cerevisiae gene deletion mutant collection, either as an ordered array or as a pool, and can be automated for high throughput.

Original languageEnglish
Title of host publicationMethods in Enzymology
EditorsJL Campbell, P Modrich
PublisherAcademic Press
Number of pages23
ISBN (Print)0-12-182814-X
Publication statusPublished - 2006
Externally publishedYes

Publication series

NameMethods in Enzymology
ISSN (Print)0076-6879


  • Base Sequence, DNA Damage, DNA Primers, Gene Deletion, Genes, Reporter, Genome, Fungal, Mutation, Polymerase Chain Reaction, Saccharomyces cerevisiae

ID: 34776363