Genomic approaches for identifying DNA damage response pathways in S-cerevisiaeChang, M., Parsons, A. B., Sheikh, B. H., Boone, C. & Brown, G. W., 2006, Methods in Enzymology. Campbell, JL. & Modrich, P. (eds.). Academic Press, Vol. 409. p. 213-235 23 p. (Methods in Enzymology; vol. 409).
Research output: Chapter in Book/Report/Conference proceeding › Chapter › Academic
DNA damage response pathways have been studied extensively in the budding yeast Saccharomyces cerevisiae, yet new genes with roles in the DNA damage response are still being identified. In this chapter we describe the use of functional genomic approaches in the identification of DNA damage response genes and pathways. These techniques take advantage of the S. cerevisiae gene deletion mutant collection, either as an ordered array or as a pool, and can be automated for high throughput.
|Title of host publication||Methods in Enzymology|
|Editors||JL Campbell, P Modrich|
|Number of pages||23|
|Publication status||Published - 2006|
|Name||Methods in Enzymology|
|Publisher||ELSEVIER ACADEMIC PRESS INC|
- Base Sequence, DNA Damage, DNA Primers, Gene Deletion, Genes, Reporter, Genome, Fungal, Mutation, Polymerase Chain Reaction, Saccharomyces cerevisiae