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Genetic features of livestock-associated Staphylococcus aureus ST9 isolates from Chinese pigs that carry the lsa(E) gene for quinupristin/dalfopristin resistance

Yan, X., Li, Z., Chlebowicz, M. A., Tao, X., Ni, M., Hu, Y., Li, Z., Grundmann, H., Murray, S., Pascoe, B., Sheppard, S. K., Bo, X., van Dijl, J. M., Du, P., Zhang, M., You, Y., Yu, X., Meng, F., Wang, S. & Zhang, J., Dec-2016, In : International journal of medical microbiology. 306, 8, p. 722-729 8 p.

Research output: Contribution to journalArticleAcademicpeer-review

Whole-genome sequencing (WGS) was used to investigate the genetic features of the recently identified lsa(E) gene in porcine S. aureus ST9 isolates. Three quinupristin/dalfopristin-resistant isolates harboring the lsa(E) gene (two MRSA and one MSSA) were sequenced. Phylogenetic analysis of 184 S. aureus genomes showed that ST9 porcine isolates belong to a distinct sequence cluster. Further analysis showed that all isolates were deficient in the recently described type IV restriction-modification system and SCCmec type XII was identified in the two MRSA isolates, which included a rare class C2 mec gene complex. A 24 kb Psi SCC fragment was found in the MRSA and MSSA isolates sharing 99% nucleotide sequence homology with the Psi SCCJCSC6690 (O-2) element of a ST9 MRSA isolate from Thailand (accession number AB705453). Comparison of these ST9 isolates with 181 publically available S. aureus genomes identified 24 genes present in all (100%) ST9 isolates, that were absent from the most closely related human isolate. Our analysis suggests that the sequenced quinupristin/dalfopristin-resistant ST9 lineage represent a reservoir of mobile genetic elements associated with resistance and virulence features. (C) 2016 Elsevier GmbH. All rights reserved.

Original languageEnglish
Pages (from-to)722-729
Number of pages8
JournalInternational journal of medical microbiology
Volume306
Issue number8
Publication statusPublished - Dec-2016

    Keywords

  • Staphylococcus aureus, Quinupristin/dalfopristin, Resistance, Livestock-associated, ST9, ABC transporters, COAGULASE-NEGATIVE STAPHYLOCOCCI, PLEUROMUTILIN-LINCOSAMIDE-STREPTOGRAMIN, ABC TRANSPORTER GENE, SWINE ORIGIN, METHICILLIN RESISTANCE, ANTIBIOTIC-RESISTANCE, CONFERS RESISTANCE, CLINICAL ISOLATE, PORCINE ORIGIN, ST398

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