Publication

Generally Applicable Transformation Protocols for Fluorescent Nanodiamond Internalization into Cells

Hemelaar, S. R., van der Laan, K. J., Hinterding, S. R., Koot, M. V., Ellermann, E., Perona-Martinez, F. P., Roig, D., Hommelet, S., Novarina, D., Takahashi, H., Chang, M. & Schirhagl, R., 19-Jul-2017, In : Scientific Reports. 7, 7 p., 5862.

Research output: Contribution to journalArticleAcademicpeer-review

APA

Hemelaar, S. R., van der Laan, K. J., Hinterding, S. R., Koot, M. V., Ellermann, E., Perona-Martinez, F. P., ... Schirhagl, R. (2017). Generally Applicable Transformation Protocols for Fluorescent Nanodiamond Internalization into Cells. Scientific Reports, 7, [5862]. https://doi.org/10.1038/s41598-017-06180-5

Author

Hemelaar, Simon R ; van der Laan, Kiran J ; Hinterding, Sophie R ; Koot, Manon V ; Ellermann, Else ; Perona-Martinez, Felipe P ; Roig, David ; Hommelet, Severin ; Novarina, Daniele ; Takahashi, Hiroki ; Chang, Michael ; Schirhagl, Romana. / Generally Applicable Transformation Protocols for Fluorescent Nanodiamond Internalization into Cells. In: Scientific Reports. 2017 ; Vol. 7.

Harvard

Hemelaar, SR, van der Laan, KJ, Hinterding, SR, Koot, MV, Ellermann, E, Perona-Martinez, FP, Roig, D, Hommelet, S, Novarina, D, Takahashi, H, Chang, M & Schirhagl, R 2017, 'Generally Applicable Transformation Protocols for Fluorescent Nanodiamond Internalization into Cells', Scientific Reports, vol. 7, 5862. https://doi.org/10.1038/s41598-017-06180-5

Standard

Generally Applicable Transformation Protocols for Fluorescent Nanodiamond Internalization into Cells. / Hemelaar, Simon R; van der Laan, Kiran J; Hinterding, Sophie R; Koot, Manon V; Ellermann, Else; Perona-Martinez, Felipe P; Roig, David; Hommelet, Severin; Novarina, Daniele; Takahashi, Hiroki; Chang, Michael; Schirhagl, Romana.

In: Scientific Reports, Vol. 7, 5862, 19.07.2017.

Research output: Contribution to journalArticleAcademicpeer-review

Vancouver

Hemelaar SR, van der Laan KJ, Hinterding SR, Koot MV, Ellermann E, Perona-Martinez FP et al. Generally Applicable Transformation Protocols for Fluorescent Nanodiamond Internalization into Cells. Scientific Reports. 2017 Jul 19;7. 5862. https://doi.org/10.1038/s41598-017-06180-5


BibTeX

@article{33ff1d4587784f6db0c1416ace1cfa5f,
title = "Generally Applicable Transformation Protocols for Fluorescent Nanodiamond Internalization into Cells",
abstract = "Fluorescent nanodiamonds (FNDs) are promising nanoprobes, owing to their stable and magnetosensitive fluorescence. Therefore they can probe properties as magnetic resonances, pressure, temperature or strain. The unprecedented sensitivity of diamond defects can detect the faint magnetic resonance of a single electron or even a few nuclear spins. However, these sensitivities are only achieved if the diamond probe is close to the molecules that need to be detected. In order to utilize its full potential for biological applications, the diamond particle has to enter the cell. Some model systems, like HeLa cells, readily ingest particles. However, most cells do not show this behavior. In this article we show for the first time generally applicable methods, which are able to transport fluorescent nanodiamonds into cells with a thick cell wall. Yeast cells, in particular Saccharomyces cerevisiae, are a favored model organism to study intracellular processes including aging on a cellular level. In order to introduce FNDs in these cells, we evaluated electrical transformation and conditions of chemical permeabilization for uptake efficiency and viability. 5{\%} DMSO (dimethyl sulfoxide) in combination with optimized chemical transformation mix leads to high uptake efficiency in combination with low impact on cell biology. We have evaluated all steps in the procedure.",
keywords = "AMBIENT CONDITIONS, YEAST, NANOPARTICLES",
author = "Hemelaar, {Simon R} and {van der Laan}, {Kiran J} and Hinterding, {Sophie R} and Koot, {Manon V} and Else Ellermann and Perona-Martinez, {Felipe P} and David Roig and Severin Hommelet and Daniele Novarina and Hiroki Takahashi and Michael Chang and Romana Schirhagl",
year = "2017",
month = "7",
day = "19",
doi = "10.1038/s41598-017-06180-5",
language = "English",
volume = "7",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",

}

RIS

TY - JOUR

T1 - Generally Applicable Transformation Protocols for Fluorescent Nanodiamond Internalization into Cells

AU - Hemelaar, Simon R

AU - van der Laan, Kiran J

AU - Hinterding, Sophie R

AU - Koot, Manon V

AU - Ellermann, Else

AU - Perona-Martinez, Felipe P

AU - Roig, David

AU - Hommelet, Severin

AU - Novarina, Daniele

AU - Takahashi, Hiroki

AU - Chang, Michael

AU - Schirhagl, Romana

PY - 2017/7/19

Y1 - 2017/7/19

N2 - Fluorescent nanodiamonds (FNDs) are promising nanoprobes, owing to their stable and magnetosensitive fluorescence. Therefore they can probe properties as magnetic resonances, pressure, temperature or strain. The unprecedented sensitivity of diamond defects can detect the faint magnetic resonance of a single electron or even a few nuclear spins. However, these sensitivities are only achieved if the diamond probe is close to the molecules that need to be detected. In order to utilize its full potential for biological applications, the diamond particle has to enter the cell. Some model systems, like HeLa cells, readily ingest particles. However, most cells do not show this behavior. In this article we show for the first time generally applicable methods, which are able to transport fluorescent nanodiamonds into cells with a thick cell wall. Yeast cells, in particular Saccharomyces cerevisiae, are a favored model organism to study intracellular processes including aging on a cellular level. In order to introduce FNDs in these cells, we evaluated electrical transformation and conditions of chemical permeabilization for uptake efficiency and viability. 5% DMSO (dimethyl sulfoxide) in combination with optimized chemical transformation mix leads to high uptake efficiency in combination with low impact on cell biology. We have evaluated all steps in the procedure.

AB - Fluorescent nanodiamonds (FNDs) are promising nanoprobes, owing to their stable and magnetosensitive fluorescence. Therefore they can probe properties as magnetic resonances, pressure, temperature or strain. The unprecedented sensitivity of diamond defects can detect the faint magnetic resonance of a single electron or even a few nuclear spins. However, these sensitivities are only achieved if the diamond probe is close to the molecules that need to be detected. In order to utilize its full potential for biological applications, the diamond particle has to enter the cell. Some model systems, like HeLa cells, readily ingest particles. However, most cells do not show this behavior. In this article we show for the first time generally applicable methods, which are able to transport fluorescent nanodiamonds into cells with a thick cell wall. Yeast cells, in particular Saccharomyces cerevisiae, are a favored model organism to study intracellular processes including aging on a cellular level. In order to introduce FNDs in these cells, we evaluated electrical transformation and conditions of chemical permeabilization for uptake efficiency and viability. 5% DMSO (dimethyl sulfoxide) in combination with optimized chemical transformation mix leads to high uptake efficiency in combination with low impact on cell biology. We have evaluated all steps in the procedure.

KW - AMBIENT CONDITIONS

KW - YEAST

KW - NANOPARTICLES

U2 - 10.1038/s41598-017-06180-5

DO - 10.1038/s41598-017-06180-5

M3 - Article

VL - 7

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

M1 - 5862

ER -

ID: 46956938