Publication

Gene silencing pathways found in the green alga Volvox carteri reveal insights into evolution and origins of small RNA systems in plants

Dueck, A., Evers, M., Henz, S. R., Unger, K., Eichner, N., Merkl, R., Berezikov, E., Engelmann, J. C., Weigel, D., Wenzl, S. & Meister, G., 2-Nov-2016, In : BMC Genomics. 17, 15 p., 853.

Research output: Contribution to journalArticleAcademicpeer-review

APA

Dueck, A., Evers, M., Henz, S. R., Unger, K., Eichner, N., Merkl, R., Berezikov, E., Engelmann, J. C., Weigel, D., Wenzl, S., & Meister, G. (2016). Gene silencing pathways found in the green alga Volvox carteri reveal insights into evolution and origins of small RNA systems in plants. BMC Genomics, 17, [853]. https://doi.org/10.1186/s12864-016-3202-4

Author

Dueck, Anne ; Evers, Maurits ; Henz, Stefan R. ; Unger, Katharina ; Eichner, Norbert ; Merkl, Rainer ; Berezikov, Eugene ; Engelmann, Julia C. ; Weigel, Detlef ; Wenzl, Stephan ; Meister, Gunter. / Gene silencing pathways found in the green alga Volvox carteri reveal insights into evolution and origins of small RNA systems in plants. In: BMC Genomics. 2016 ; Vol. 17.

Harvard

Dueck, A, Evers, M, Henz, SR, Unger, K, Eichner, N, Merkl, R, Berezikov, E, Engelmann, JC, Weigel, D, Wenzl, S & Meister, G 2016, 'Gene silencing pathways found in the green alga Volvox carteri reveal insights into evolution and origins of small RNA systems in plants', BMC Genomics, vol. 17, 853. https://doi.org/10.1186/s12864-016-3202-4

Standard

Gene silencing pathways found in the green alga Volvox carteri reveal insights into evolution and origins of small RNA systems in plants. / Dueck, Anne; Evers, Maurits; Henz, Stefan R.; Unger, Katharina; Eichner, Norbert; Merkl, Rainer; Berezikov, Eugene; Engelmann, Julia C.; Weigel, Detlef; Wenzl, Stephan; Meister, Gunter.

In: BMC Genomics, Vol. 17, 853, 02.11.2016.

Research output: Contribution to journalArticleAcademicpeer-review

Vancouver

Dueck A, Evers M, Henz SR, Unger K, Eichner N, Merkl R et al. Gene silencing pathways found in the green alga Volvox carteri reveal insights into evolution and origins of small RNA systems in plants. BMC Genomics. 2016 Nov 2;17. 853. https://doi.org/10.1186/s12864-016-3202-4


BibTeX

@article{c85867420b4e4346895afa634b99406a,
title = "Gene silencing pathways found in the green alga Volvox carteri reveal insights into evolution and origins of small RNA systems in plants",
abstract = "Background: Volvox carteri (V. carteri) is a multicellular green alga used as model system for the evolution of multicellularity. So far, the contribution of small RNA pathways to these phenomena is not understood. Thus, we have sequenced V. carteri Argonaute 3 (VcAGO3)-associated small RNAs from different developmental stages.Results: Using this functional approach, we define the Volvox microRNA (miRNA) repertoire and show that miRNAs are not conserved in the closely related unicellular alga Chlamydomonas reinhardtii. Furthermore, we find that miRNAs are differentially expressed during different life stages of V. carteri. In addition to miRNAs, transposon-associated small RNAs or phased siRNA loci, which are common in higher land plants, are highly abundant in Volvox as well. Transposons not only give rise to miRNAs and other small RNAs, they are also targets of small RNAs.Conclusion: Our analyses reveal a surprisingly complex small RNA network in Volvox as elaborate as in higher land plants. At least the identified VcAGO3-associated miRNAs are not conserved in C. reinhardtii suggesting fast evolution of small RNA systems. Thus, distinct small RNAs may contribute to multicellularity and also division of labor in reproductive and somatic cells.",
keywords = "microRNAs, Gene silencing, Transposons, Small RNAs, Evolution, Argonaute, Volvox carteri, HUMAN ARGONAUTE PROTEINS, CHLAMYDOMONAS-REINHARDTII, MIRNAS, ARABIDOPSIS, DATABASE, SIRNAS, PREDICTION, MICRORNA, ELEMENTS, CLASSIFICATION",
author = "Anne Dueck and Maurits Evers and Henz, {Stefan R.} and Katharina Unger and Norbert Eichner and Rainer Merkl and Eugene Berezikov and Engelmann, {Julia C.} and Detlef Weigel and Stephan Wenzl and Gunter Meister",
year = "2016",
month = nov,
day = "2",
doi = "10.1186/s12864-016-3202-4",
language = "English",
volume = "17",
journal = "BMC Genomics",
issn = "1471-2164",
publisher = "BioMed Central Ltd.",

}

RIS

TY - JOUR

T1 - Gene silencing pathways found in the green alga Volvox carteri reveal insights into evolution and origins of small RNA systems in plants

AU - Dueck, Anne

AU - Evers, Maurits

AU - Henz, Stefan R.

AU - Unger, Katharina

AU - Eichner, Norbert

AU - Merkl, Rainer

AU - Berezikov, Eugene

AU - Engelmann, Julia C.

AU - Weigel, Detlef

AU - Wenzl, Stephan

AU - Meister, Gunter

PY - 2016/11/2

Y1 - 2016/11/2

N2 - Background: Volvox carteri (V. carteri) is a multicellular green alga used as model system for the evolution of multicellularity. So far, the contribution of small RNA pathways to these phenomena is not understood. Thus, we have sequenced V. carteri Argonaute 3 (VcAGO3)-associated small RNAs from different developmental stages.Results: Using this functional approach, we define the Volvox microRNA (miRNA) repertoire and show that miRNAs are not conserved in the closely related unicellular alga Chlamydomonas reinhardtii. Furthermore, we find that miRNAs are differentially expressed during different life stages of V. carteri. In addition to miRNAs, transposon-associated small RNAs or phased siRNA loci, which are common in higher land plants, are highly abundant in Volvox as well. Transposons not only give rise to miRNAs and other small RNAs, they are also targets of small RNAs.Conclusion: Our analyses reveal a surprisingly complex small RNA network in Volvox as elaborate as in higher land plants. At least the identified VcAGO3-associated miRNAs are not conserved in C. reinhardtii suggesting fast evolution of small RNA systems. Thus, distinct small RNAs may contribute to multicellularity and also division of labor in reproductive and somatic cells.

AB - Background: Volvox carteri (V. carteri) is a multicellular green alga used as model system for the evolution of multicellularity. So far, the contribution of small RNA pathways to these phenomena is not understood. Thus, we have sequenced V. carteri Argonaute 3 (VcAGO3)-associated small RNAs from different developmental stages.Results: Using this functional approach, we define the Volvox microRNA (miRNA) repertoire and show that miRNAs are not conserved in the closely related unicellular alga Chlamydomonas reinhardtii. Furthermore, we find that miRNAs are differentially expressed during different life stages of V. carteri. In addition to miRNAs, transposon-associated small RNAs or phased siRNA loci, which are common in higher land plants, are highly abundant in Volvox as well. Transposons not only give rise to miRNAs and other small RNAs, they are also targets of small RNAs.Conclusion: Our analyses reveal a surprisingly complex small RNA network in Volvox as elaborate as in higher land plants. At least the identified VcAGO3-associated miRNAs are not conserved in C. reinhardtii suggesting fast evolution of small RNA systems. Thus, distinct small RNAs may contribute to multicellularity and also division of labor in reproductive and somatic cells.

KW - microRNAs

KW - Gene silencing

KW - Transposons

KW - Small RNAs

KW - Evolution

KW - Argonaute

KW - Volvox carteri

KW - HUMAN ARGONAUTE PROTEINS

KW - CHLAMYDOMONAS-REINHARDTII

KW - MIRNAS

KW - ARABIDOPSIS

KW - DATABASE

KW - SIRNAS

KW - PREDICTION

KW - MICRORNA

KW - ELEMENTS

KW - CLASSIFICATION

U2 - 10.1186/s12864-016-3202-4

DO - 10.1186/s12864-016-3202-4

M3 - Article

VL - 17

JO - BMC Genomics

JF - BMC Genomics

SN - 1471-2164

M1 - 853

ER -

ID: 37451057