Publication

Gene network approach reveals co-expression patterns in nasal and bronchial epithelium

Imkamp, K., Bernal, V., Grzegorzcyk, M., Horvatovich, P., Vermeulen, C. J., Heijink, I. H., Guryev, V., Kerstjens, H. A. M., van den Berge, M. & Faiz, A., 1-Nov-2019, In : Scientific Reports. 9, 1, 13 p., 15835.

Research output: Contribution to journalArticleAcademicpeer-review

APA

Imkamp, K., Bernal, V., Grzegorzcyk, M., Horvatovich, P., Vermeulen, C. J., Heijink, I. H., ... Faiz, A. (2019). Gene network approach reveals co-expression patterns in nasal and bronchial epithelium. Scientific Reports, 9(1), [15835]. https://doi.org/10.1038/s41598-019-50963-x

Author

Imkamp, Kai ; Bernal, Victor ; Grzegorzcyk, Marco ; Horvatovich, Peter ; Vermeulen, Cornelis J ; Heijink, Irene H ; Guryev, Victor ; Kerstjens, Huib A M ; van den Berge, Maarten ; Faiz, Alen. / Gene network approach reveals co-expression patterns in nasal and bronchial epithelium. In: Scientific Reports. 2019 ; Vol. 9, No. 1.

Harvard

Imkamp, K, Bernal, V, Grzegorzcyk, M, Horvatovich, P, Vermeulen, CJ, Heijink, IH, Guryev, V, Kerstjens, HAM, van den Berge, M & Faiz, A 2019, 'Gene network approach reveals co-expression patterns in nasal and bronchial epithelium', Scientific Reports, vol. 9, no. 1, 15835. https://doi.org/10.1038/s41598-019-50963-x

Standard

Gene network approach reveals co-expression patterns in nasal and bronchial epithelium. / Imkamp, Kai; Bernal, Victor; Grzegorzcyk, Marco; Horvatovich, Peter; Vermeulen, Cornelis J; Heijink, Irene H; Guryev, Victor; Kerstjens, Huib A M; van den Berge, Maarten; Faiz, Alen.

In: Scientific Reports, Vol. 9, No. 1, 15835, 01.11.2019.

Research output: Contribution to journalArticleAcademicpeer-review

Vancouver

Imkamp K, Bernal V, Grzegorzcyk M, Horvatovich P, Vermeulen CJ, Heijink IH et al. Gene network approach reveals co-expression patterns in nasal and bronchial epithelium. Scientific Reports. 2019 Nov 1;9(1). 15835. https://doi.org/10.1038/s41598-019-50963-x


BibTeX

@article{30e78a17819f4c118c83c437672af034,
title = "Gene network approach reveals co-expression patterns in nasal and bronchial epithelium",
abstract = "Nasal gene expression profiling is a new approach to investigate the airway epithelium as a biomarker to study the activity and treatment responses of obstructive pulmonary diseases. We investigated to what extent gene expression profiling of nasal brushings is similar to that of bronchial brushings. We performed genome wide gene expression profiling on matched nasal and bronchial epithelial brushes from 77 respiratory healthy individuals. To investigate differences and similarities among regulatory modules, network analysis was performed on correlated, differentially expressed and smoking-related genes using Gaussian Graphical Models. Between nasal and bronchial brushes, 619 genes were correlated and 1692 genes were differentially expressed (false discovery rate <0.05, |Fold-change|>2). Network analysis of correlated genes showed pro-inflammatory pathways to be similar between the two locations. Focusing on smoking-related genes, cytochrome-P450 pathway related genes were found to be similar, supporting the concept of a detoxifying response to tobacco exposure throughout the airways. In contrast, cilia-related pathways were decreased in nasal compared to bronchial brushes when focusing on differentially expressed genes. Collectively, while there are substantial differences in gene expression between nasal and bronchial brushes, we also found similarities, especially in the response to the external factors such as smoking.",
author = "Kai Imkamp and Victor Bernal and Marco Grzegorzcyk and Peter Horvatovich and Vermeulen, {Cornelis J} and Heijink, {Irene H} and Victor Guryev and Kerstjens, {Huib A M} and {van den Berge}, Maarten and Alen Faiz",
year = "2019",
month = "11",
day = "1",
doi = "10.1038/s41598-019-50963-x",
language = "English",
volume = "9",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",
number = "1",

}

RIS

TY - JOUR

T1 - Gene network approach reveals co-expression patterns in nasal and bronchial epithelium

AU - Imkamp, Kai

AU - Bernal, Victor

AU - Grzegorzcyk, Marco

AU - Horvatovich, Peter

AU - Vermeulen, Cornelis J

AU - Heijink, Irene H

AU - Guryev, Victor

AU - Kerstjens, Huib A M

AU - van den Berge, Maarten

AU - Faiz, Alen

PY - 2019/11/1

Y1 - 2019/11/1

N2 - Nasal gene expression profiling is a new approach to investigate the airway epithelium as a biomarker to study the activity and treatment responses of obstructive pulmonary diseases. We investigated to what extent gene expression profiling of nasal brushings is similar to that of bronchial brushings. We performed genome wide gene expression profiling on matched nasal and bronchial epithelial brushes from 77 respiratory healthy individuals. To investigate differences and similarities among regulatory modules, network analysis was performed on correlated, differentially expressed and smoking-related genes using Gaussian Graphical Models. Between nasal and bronchial brushes, 619 genes were correlated and 1692 genes were differentially expressed (false discovery rate <0.05, |Fold-change|>2). Network analysis of correlated genes showed pro-inflammatory pathways to be similar between the two locations. Focusing on smoking-related genes, cytochrome-P450 pathway related genes were found to be similar, supporting the concept of a detoxifying response to tobacco exposure throughout the airways. In contrast, cilia-related pathways were decreased in nasal compared to bronchial brushes when focusing on differentially expressed genes. Collectively, while there are substantial differences in gene expression between nasal and bronchial brushes, we also found similarities, especially in the response to the external factors such as smoking.

AB - Nasal gene expression profiling is a new approach to investigate the airway epithelium as a biomarker to study the activity and treatment responses of obstructive pulmonary diseases. We investigated to what extent gene expression profiling of nasal brushings is similar to that of bronchial brushings. We performed genome wide gene expression profiling on matched nasal and bronchial epithelial brushes from 77 respiratory healthy individuals. To investigate differences and similarities among regulatory modules, network analysis was performed on correlated, differentially expressed and smoking-related genes using Gaussian Graphical Models. Between nasal and bronchial brushes, 619 genes were correlated and 1692 genes were differentially expressed (false discovery rate <0.05, |Fold-change|>2). Network analysis of correlated genes showed pro-inflammatory pathways to be similar between the two locations. Focusing on smoking-related genes, cytochrome-P450 pathway related genes were found to be similar, supporting the concept of a detoxifying response to tobacco exposure throughout the airways. In contrast, cilia-related pathways were decreased in nasal compared to bronchial brushes when focusing on differentially expressed genes. Collectively, while there are substantial differences in gene expression between nasal and bronchial brushes, we also found similarities, especially in the response to the external factors such as smoking.

U2 - 10.1038/s41598-019-50963-x

DO - 10.1038/s41598-019-50963-x

M3 - Article

VL - 9

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

IS - 1

M1 - 15835

ER -

ID: 101214061