GD1a Overcomes Inhibition of Myelination by Fibronectin via Activation of Protein Kinase A: Implications for Multiple SclerosisQin, J., Sikkema, A. H., van der Bij, K., de Jonge, J. C., Klappe, K., Nies, V., Jonker, J. W., Kok, J. W., Hoekstra, D. & Baron, W., 11-Oct-2017, In : The Journal of Neuroscience. 37, 41, p. 9925-9938 14 p.
Research output: Contribution to journal › Article › Academic › peer-review
Remyelination failure by oligodendrocytes contributes to the functional impairment that characterizes the demyelinating disease multiple sclerosis (MS). Since incomplete remyelination will irreversibly damage axonal connections, treatments effectively promoting remyelination are pivotal in halting disease progression. Our previous findings suggest that fibronectin aggregates, as an environmental factor, contribute to remyelination failure by perturbing oligodendrocyte progenitor cell (OPC) maturation. Here, we aim at elucidating whether exogenously added gangliosides (i.e., cell surface lipids with a potential to modulate signaling pathways) could counteract fibronectin-mediated inhibition of OPC maturation. Exclusive exposure of rat oligodendrocytes to GD1a, but not other gangliosides, overcomes aggregated fibronectin-induced inhibition of myelin membrane formation, in vitro, and OPC differentiation in fibronectin aggregate containing cuprizone-induced demyelinated lesions in male mice. GD1a exerts its effect on OPCs by inducing their proliferation and, at a late stage, by modulating OPC maturation. Kinase activity profiling revealed that GD1a activated a protein kinase A (PKA)-dependent signaling pathway and increased phosphorylation of the transcription factorcAMPresponse element-binding protein. Consistently, the effect of GD1a in restoring myelin membrane formation in the presence of fibronectin aggregates was abolished by the PKA inhibitor H89, whereas the effect of GD1a was mimicked by the PKA activator dibutyryl-cAMP. Together, GD1a overcomes the inhibiting effect of aggregated fibronectin on OPC maturation by activating a PKA-dependent signaling pathway. Given the persistent presence of fibronectin aggregates in MS lesions, ganglioside GD1a might act as a potential novel therapeutic tool to selectively modulate the detrimental signaling environment that precludes remyelination.
|Number of pages||14|
|Journal||The Journal of Neuroscience|
|Early online date||12-Sep-2017|
|Publication status||Published - 11-Oct-2017|
- fibronectin, ganglioside, multiple sclerosis, oligodendrocyte, remyelination, OLIGODENDROCYTE PROGENITOR CELLS, CENTRAL-NERVOUS-SYSTEM, CYCLIC-AMP, DEMYELINATED LESIONS, MEMBRANE FORMATION, ANCHORED PROTEINS, RAFT-ASSOCIATION, PRECURSOR CELLS, IN-VITRO, DIFFERENTIATION