Publication

Functional analysis of the Ala67Thr polymorphism in agouti related protein associated with anorexia nervosa and leanness

de Rijke, C. E., Jackson, P. J., Garner, K. M., van Rozen, R. J., Douglas, N. R., Kas, M. J. H., Millhauser, G. L. & Adan, R. A. H., 15-Jul-2005, In : Biochemical Pharmacology. 70, 2, p. 308-16 9 p.

Research output: Contribution to journalArticleAcademicpeer-review

APA

de Rijke, C. E., Jackson, P. J., Garner, K. M., van Rozen, R. J., Douglas, N. R., Kas, M. J. H., ... Adan, R. A. H. (2005). Functional analysis of the Ala67Thr polymorphism in agouti related protein associated with anorexia nervosa and leanness. Biochemical Pharmacology, 70(2), 308-16. https://doi.org/10.1016/j.bcp.2005.04.033

Author

de Rijke, Corine E ; Jackson, Pilgrim J ; Garner, Keith M ; van Rozen, Rea J ; Douglas, Nick R ; Kas, Martien J H ; Millhauser, Glenn L ; Adan, Roger A H. / Functional analysis of the Ala67Thr polymorphism in agouti related protein associated with anorexia nervosa and leanness. In: Biochemical Pharmacology. 2005 ; Vol. 70, No. 2. pp. 308-16.

Harvard

de Rijke, CE, Jackson, PJ, Garner, KM, van Rozen, RJ, Douglas, NR, Kas, MJH, Millhauser, GL & Adan, RAH 2005, 'Functional analysis of the Ala67Thr polymorphism in agouti related protein associated with anorexia nervosa and leanness', Biochemical Pharmacology, vol. 70, no. 2, pp. 308-16. https://doi.org/10.1016/j.bcp.2005.04.033

Standard

Functional analysis of the Ala67Thr polymorphism in agouti related protein associated with anorexia nervosa and leanness. / de Rijke, Corine E; Jackson, Pilgrim J; Garner, Keith M; van Rozen, Rea J; Douglas, Nick R; Kas, Martien J H; Millhauser, Glenn L; Adan, Roger A H.

In: Biochemical Pharmacology, Vol. 70, No. 2, 15.07.2005, p. 308-16.

Research output: Contribution to journalArticleAcademicpeer-review

Vancouver

de Rijke CE, Jackson PJ, Garner KM, van Rozen RJ, Douglas NR, Kas MJH et al. Functional analysis of the Ala67Thr polymorphism in agouti related protein associated with anorexia nervosa and leanness. Biochemical Pharmacology. 2005 Jul 15;70(2):308-16. https://doi.org/10.1016/j.bcp.2005.04.033


BibTeX

@article{f00ef8e99c47439298e29062c7244b95,
title = "Functional analysis of the Ala67Thr polymorphism in agouti related protein associated with anorexia nervosa and leanness",
abstract = "AgRP is a neuropeptide that stimulates food intake through inhibition of central melanocortin receptors (MCRs). In humans, the non-conservative amino acid substitution Alanine (Ala) 67 Threonine (Thr) has been associated with Anorexia Nervosa and with leanness. In the present study, the cellular distribution, processing and in vitro and in vivo activities of Ala67 and Thr67 AgRP were investigated. Western blots of media and lysates of BHK cells stably transfected with Ala67 or Thr67 expression constructs showed identical AgRP bands. Both Ala67 and Thr67 AgRP colocalised with the Golgi apparatus, but not with the ER or lysosomes when expressed in Att20 D16V cells. Also, no differences were observed between the potencies of bacterially expressed Ala67 and Thr67 AgRP to stimulate MC4R in a reporter gene assay or inhibit food intake in rats. Taken together, no evidence was found for a functional defect of Thr67 AgRP related to MC4R interactions.",
keywords = "Agouti-Related Protein, Alanine, Animals, Anorexia Nervosa, Cell Line, Cricetinae, Dose-Response Relationship, Drug, Eating, Humans, Injections, Intraventricular, Intercellular Signaling Peptides and Proteins, Polymorphism, Genetic, Proteins, Rats, Rats, Wistar, Thinness, Threonine",
author = "{de Rijke}, {Corine E} and Jackson, {Pilgrim J} and Garner, {Keith M} and {van Rozen}, {Rea J} and Douglas, {Nick R} and Kas, {Martien J H} and Millhauser, {Glenn L} and Adan, {Roger A H}",
year = "2005",
month = "7",
day = "15",
doi = "10.1016/j.bcp.2005.04.033",
language = "English",
volume = "70",
pages = "308--16",
journal = "Biochemical Pharmacology",
issn = "0006-2952",
publisher = "PERGAMON-ELSEVIER SCIENCE LTD",
number = "2",

}

RIS

TY - JOUR

T1 - Functional analysis of the Ala67Thr polymorphism in agouti related protein associated with anorexia nervosa and leanness

AU - de Rijke, Corine E

AU - Jackson, Pilgrim J

AU - Garner, Keith M

AU - van Rozen, Rea J

AU - Douglas, Nick R

AU - Kas, Martien J H

AU - Millhauser, Glenn L

AU - Adan, Roger A H

PY - 2005/7/15

Y1 - 2005/7/15

N2 - AgRP is a neuropeptide that stimulates food intake through inhibition of central melanocortin receptors (MCRs). In humans, the non-conservative amino acid substitution Alanine (Ala) 67 Threonine (Thr) has been associated with Anorexia Nervosa and with leanness. In the present study, the cellular distribution, processing and in vitro and in vivo activities of Ala67 and Thr67 AgRP were investigated. Western blots of media and lysates of BHK cells stably transfected with Ala67 or Thr67 expression constructs showed identical AgRP bands. Both Ala67 and Thr67 AgRP colocalised with the Golgi apparatus, but not with the ER or lysosomes when expressed in Att20 D16V cells. Also, no differences were observed between the potencies of bacterially expressed Ala67 and Thr67 AgRP to stimulate MC4R in a reporter gene assay or inhibit food intake in rats. Taken together, no evidence was found for a functional defect of Thr67 AgRP related to MC4R interactions.

AB - AgRP is a neuropeptide that stimulates food intake through inhibition of central melanocortin receptors (MCRs). In humans, the non-conservative amino acid substitution Alanine (Ala) 67 Threonine (Thr) has been associated with Anorexia Nervosa and with leanness. In the present study, the cellular distribution, processing and in vitro and in vivo activities of Ala67 and Thr67 AgRP were investigated. Western blots of media and lysates of BHK cells stably transfected with Ala67 or Thr67 expression constructs showed identical AgRP bands. Both Ala67 and Thr67 AgRP colocalised with the Golgi apparatus, but not with the ER or lysosomes when expressed in Att20 D16V cells. Also, no differences were observed between the potencies of bacterially expressed Ala67 and Thr67 AgRP to stimulate MC4R in a reporter gene assay or inhibit food intake in rats. Taken together, no evidence was found for a functional defect of Thr67 AgRP related to MC4R interactions.

KW - Agouti-Related Protein

KW - Alanine

KW - Animals

KW - Anorexia Nervosa

KW - Cell Line

KW - Cricetinae

KW - Dose-Response Relationship, Drug

KW - Eating

KW - Humans

KW - Injections, Intraventricular

KW - Intercellular Signaling Peptides and Proteins

KW - Polymorphism, Genetic

KW - Proteins

KW - Rats

KW - Rats, Wistar

KW - Thinness

KW - Threonine

U2 - 10.1016/j.bcp.2005.04.033

DO - 10.1016/j.bcp.2005.04.033

M3 - Article

VL - 70

SP - 308

EP - 316

JO - Biochemical Pharmacology

JF - Biochemical Pharmacology

SN - 0006-2952

IS - 2

ER -

ID: 32495652