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Fully automatized high-throughput enzyme library screening using a robotic platform

Doerr, M., Fibinger, M. P. C., Last, D., Schmidt, S., Santos-Aberturas, J., Boettcher, D., Hummel, A., Vickers, C., Voss, M. & Bornscheuer, U. T., Jul-2016, In : Biotechnology and Bioengineering. 113, 7, p. 1421-1432 12 p.

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  • Fully automatized high‐throughput enzyme library screening using a robotic platform

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DOI

  • Mark Doerr
  • Michael P. C. Fibinger
  • Daniel Last
  • Sandy Schmidt
  • Javier Santos-Aberturas
  • Dominique Boettcher
  • Anke Hummel
  • Clare Vickers
  • Moritz Voss
  • Uwe T. Bornscheuer

A fully automatized robotic platform has been established to facilitate high-throughput screening for protein engineering purposes. This platform enables proper monitoring and control of growth conditions in the microtiter plate format to ensure precise enzyme production for the interrogation of enzyme mutant libraries, protein stability tests and multiple assay screenings. The performance of this system has been exemplified for four enzyme classes important for biocatalysis such as Baeyer-Villiger monooxygenase, transaminase, dehalogenase and acylase in the high-throughput screening of various mutant libraries. This allowed the identification of novel enzyme variants in a sophisticated and highly reliable manner. Furthermore, the detailed optimization protocols should enable other researchers to adapt and improve their methods. Biotechnol. Bioeng. 2016;113: 1421-1432. (c) 2016 Wiley Periodicals, Inc.

Original languageEnglish
Pages (from-to)1421-1432
Number of pages12
JournalBiotechnology and Bioengineering
Volume113
Issue number7
Publication statusPublished - Jul-2016
Externally publishedYes

    Keywords

  • activity assay, enzymes, growth conditions, high-throughput screening, protein engineering, robotic platform, PURIFICATION, SELECTION, DESIGN, ASSAY

ID: 131274343