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From the wound to the bench: exoproteome interplay between wound-colonizing Staphylococcus aureus strains and co-existing bacteria

García-Pérez, A. N., de Jong, A., Junker, S., Becher, D., Chlebowicz, M. A., Duipmans, J. C., Jonkman, M. F. & van Dijl, J. M., 2018, In : Virulence. 9, 1, p. 363-378 16 p.

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Wound-colonizing microorganisms can form complex and dynamic polymicrobial communities where pathogens and commensals may co-exist, cooperate or compete with each other. The present study was aimed at identifying possible interactions between different bacteria isolated from the same chronic wound of a patient with the genetic blistering disease epidermolysis bullosa (EB). Specifically, this involved two different isolates of the human pathogen Staphylococcus aureus, and isolates of Bacillus thuringiensis and Klebsiella oxytoca. Particular focus was attributed to interactions of S. aureus with the two other species, because of the high staphylococcal prevalence among chronic wounds. Intriguingly, upon co-cultivation, none of the wound isolates inhibited each other's growth. Since the extracellular proteome of bacterial pathogens is a reservoir of virulence factors, the exoproteomes of the staphylococcal isolates in monoculture and co-culture with B. thuringiensis and K. oxytoca were characterized by Mass Spectrometry to explore the inherent relationships between these co-exisiting bacteria. This revealed a massive reduction in the number of staphylococcal exoproteins upon co-culturing with K. oxytoca or B. thuringiensis. Interestingly, this decrease was particularly evident for extracellular proteins with a predicted cytoplasmic localization, which were recently implicated in staphylococcal virulence and epidemiology. Furthermore, our exoproteome analysis uncovered potential cooperativity between the two different S. aureus isolates. Altogether, the observed exoproteome variations upon co-culturing are indicative of unprecedented adaptive mechanisms that set limits to the production of secreted staphylococcal virulence factors.

Original languageEnglish
Pages (from-to)363-378
Number of pages16
JournalVirulence
Volume9
Issue number1
Early online date13-Dec-2017
Publication statusPublished - 2018

    Keywords

  • Journal Article, NASAL CARRIAGE, INFECTION, CELL-SURFACE PROTEOME, ELONGATION-FACTOR TU, BACILLUS-SUBTILIS, EPIDERMOLYSIS-BULLOSA, MOONLIGHTING PROTEINS, ANTIBIOTIC-RESISTANCE, ESCHERICHIA-COLI, SIGNAL PEPTIDES

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