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Frequency and distance of transposition of a modified Dissociation element in transgenic tobacco

Biezen, E. A. V. D., Cardol, E. F., Chung, H. Y., Nijkamp, H. J. J. & Hille, J., 1996, In : Transgenic Research. 5, 5, 15 p.

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  • E.A. van der Biezen
  • E.F. Cardol
  • H.Y. Chung
  • H.J.J. Nijkamp
  • J. Hille
Effective transposon tagging with the Ac/Ds system in heterologous plant species relies on the accomplishment of a potentially high transposon-induced mutation frequency. The primary parameters that determine the mutation frequency include the transposition frequency and the transposition distance. In addition, the development of a generally applicable transposon tagging strategy requires predictable transposition behaviour. We systematically analysed Ds transposition frequencies and Ds transposition distances in tobacco. An artificial Ds element was engineered with reporter genes that allowed transposon excision and integration to be monitored visually. To analyse the variability of Ds transposition between different tobacco lines, eight single copy T-DNA transformants were selected. For trans-activation of the Ds elements, different Ac lines were used carrying an unmodified Ac+ element, an immobilized sAc element and a stable Ac element under the control of a heterologous chalcone synthase (chsA) promoter. With all Ac elements, each Ds line showed characteristic and heritable variegation patterns at the seedling level. Similar Ds line-specificity was observed for the frequency by which Ds transpositions were germinally transmitted, as well as for the distances of the Ds transpositions. The sac element induced transposition of Ds late in plant development, resulting in low germinal transposition frequencies (0.37%) and high incidences of independent transposition (83%). The majority of these Ds elements (58%) transposed to genetically closed linked sites (≤10 cM).
Original languageEnglish
Number of pages15
JournalTransgenic Research
Volume5
Issue number5
Publication statusPublished - 1996

    Keywords

  • Nicotiana tabacum, position effects, visual transposition assays, Ac/Ds system, heterologous transposon tagging

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