Publication

Fragments of Citrullinated and MMP-degraded Vimentin and MMP-degraded Type III Collagen Are Novel Serological Biomarkers to Differentiate Crohn's Disease from Ulcerative Colitis

Mortensen, J. H., Godskesen, L. E., Jensen, M. D., Van Haaften, W. T., Klinge, L. G., Olinga, P., Dijkstra, G., Kjeldsen, J., Karsdal, M. A., Bay-Jensen, A-C. & Krag, A., Oct-2015, In : Journal of Crohn's and Colitis. 9, 10, p. 863-872 10 p.

Research output: Contribution to journalArticleAcademicpeer-review

APA

Mortensen, J. H., Godskesen, L. E., Jensen, M. D., Van Haaften, W. T., Klinge, L. G., Olinga, P., ... Krag, A. (2015). Fragments of Citrullinated and MMP-degraded Vimentin and MMP-degraded Type III Collagen Are Novel Serological Biomarkers to Differentiate Crohn's Disease from Ulcerative Colitis. Journal of Crohn's and Colitis, 9(10), 863-872. https://doi.org/10.1093/ecco-jcc/jjv123

Author

Mortensen, Joachim Hog ; Godskesen, Line Elbjerg ; Jensen, Michael Dam ; Van Haaften, Wouter Tobias ; Klinge, Lone Gabriels ; Olinga, Peter ; Dijkstra, Gerard ; Kjeldsen, Jens ; Karsdal, Morten Asser ; Bay-Jensen, Anne-Christine ; Krag, Aleksander. / Fragments of Citrullinated and MMP-degraded Vimentin and MMP-degraded Type III Collagen Are Novel Serological Biomarkers to Differentiate Crohn's Disease from Ulcerative Colitis. In: Journal of Crohn's and Colitis. 2015 ; Vol. 9, No. 10. pp. 863-872.

Harvard

Mortensen, JH, Godskesen, LE, Jensen, MD, Van Haaften, WT, Klinge, LG, Olinga, P, Dijkstra, G, Kjeldsen, J, Karsdal, MA, Bay-Jensen, A-C & Krag, A 2015, 'Fragments of Citrullinated and MMP-degraded Vimentin and MMP-degraded Type III Collagen Are Novel Serological Biomarkers to Differentiate Crohn's Disease from Ulcerative Colitis' Journal of Crohn's and Colitis, vol. 9, no. 10, pp. 863-872. https://doi.org/10.1093/ecco-jcc/jjv123

Standard

Fragments of Citrullinated and MMP-degraded Vimentin and MMP-degraded Type III Collagen Are Novel Serological Biomarkers to Differentiate Crohn's Disease from Ulcerative Colitis. / Mortensen, Joachim Hog; Godskesen, Line Elbjerg; Jensen, Michael Dam; Van Haaften, Wouter Tobias; Klinge, Lone Gabriels; Olinga, Peter; Dijkstra, Gerard; Kjeldsen, Jens; Karsdal, Morten Asser; Bay-Jensen, Anne-Christine; Krag, Aleksander.

In: Journal of Crohn's and Colitis, Vol. 9, No. 10, 10.2015, p. 863-872.

Research output: Contribution to journalArticleAcademicpeer-review

Vancouver

Mortensen JH, Godskesen LE, Jensen MD, Van Haaften WT, Klinge LG, Olinga P et al. Fragments of Citrullinated and MMP-degraded Vimentin and MMP-degraded Type III Collagen Are Novel Serological Biomarkers to Differentiate Crohn's Disease from Ulcerative Colitis. Journal of Crohn's and Colitis. 2015 Oct;9(10):863-872. https://doi.org/10.1093/ecco-jcc/jjv123


BibTeX

@article{84784a03fe5d4351a3cc9c494c67a949,
title = "Fragments of Citrullinated and MMP-degraded Vimentin and MMP-degraded Type III Collagen Are Novel Serological Biomarkers to Differentiate Crohn's Disease from Ulcerative Colitis",
abstract = "Background and Aims: A hallmark of inflammatory bowel disease [IBD] is chronic inflammation, which leads to excessive extracellular matrix [ECM] remodelling and release of specific protein fragments, called neoepitopes. We speculated that the biomarker profile panel for ulcerative colitis [UC] and Crohn's disease [CD] represent a heterogeneous expression pattern, and may be applied as a tool to aid in the differentiation between UC and CD.Methods: Serum biomarkers of degraded collagens I, III-IV [C1M, C3M, and C4M], collagen type 1 and IV formation [P1NP, P4NP], and citrullinated and MMP-degraded vimentin [VICM] were studied with a competitive ELISA assay system in a cohort including 164 subjects [CD n = 72, UC n = 60, and non-IBD controls n = 32] and a validation cohort of 61 subjects [CD n = 46, and UC n = 15]. Receiver operating characteristic curve analysis and logistic regression modelling were carried out to evaluate the discriminative power of the biomarkers.Results: All biomarkers were corrected for confounding factors. VICM and C3M demonstrated the highest diagnostic power, alone, to differentiate CD from UC with an area under the curve [AUC] of 0.77 and 0.69, respectively. Furthermore, the biomarkers C1M [AUC = 0.81], C3M [AUC = 0.83], VICM [AUC = 0.83], and P1NP [AUC = 0.77] were best to differentiate UC from non-IBD. The best combinations of biomarkers to differentiate CD from UC and UC from non-IBD were VICM, C3M, C4M [AUC = 0.90] and VICM, C3M [AUC = 0.98] respectively.Conclusions: Specific extracellular matrix degradation markers are elevated in IBD and can discriminate CD from UC and UC from non-IBD controls with a high diagnostic accuracy.",
keywords = "Inflammatory bowel disease, serological biomarkers, extracellular matrix remodelling, INFLAMMATORY-BOWEL-DISEASE, MATRIX METALLOPROTEINASES, ANKYLOSING-SPONDYLITIS, RHEUMATOID-ARTHRITIS, CONNECTIVE-TISSUE, EXPRESSION, ANTIBODIES, INHIBITORS, TURNOVER, MARKERS",
author = "Mortensen, {Joachim Hog} and Godskesen, {Line Elbjerg} and Jensen, {Michael Dam} and {Van Haaften}, {Wouter Tobias} and Klinge, {Lone Gabriels} and Peter Olinga and Gerard Dijkstra and Jens Kjeldsen and Karsdal, {Morten Asser} and Anne-Christine Bay-Jensen and Aleksander Krag",
note = "Copyright {\circledC} 2015 European Crohn’s and Colitis Organisation (ECCO). Published by Oxford University Press. All rights reserved. For permissions, please email: journals.permissions@oup.com.",
year = "2015",
month = "10",
doi = "10.1093/ecco-jcc/jjv123",
language = "English",
volume = "9",
pages = "863--872",
journal = "Journal of Crohn's and Colitis",
issn = "1873-9946",
publisher = "Oxford University Press",
number = "10",

}

RIS

TY - JOUR

T1 - Fragments of Citrullinated and MMP-degraded Vimentin and MMP-degraded Type III Collagen Are Novel Serological Biomarkers to Differentiate Crohn's Disease from Ulcerative Colitis

AU - Mortensen, Joachim Hog

AU - Godskesen, Line Elbjerg

AU - Jensen, Michael Dam

AU - Van Haaften, Wouter Tobias

AU - Klinge, Lone Gabriels

AU - Olinga, Peter

AU - Dijkstra, Gerard

AU - Kjeldsen, Jens

AU - Karsdal, Morten Asser

AU - Bay-Jensen, Anne-Christine

AU - Krag, Aleksander

N1 - Copyright © 2015 European Crohn’s and Colitis Organisation (ECCO). Published by Oxford University Press. All rights reserved. For permissions, please email: journals.permissions@oup.com.

PY - 2015/10

Y1 - 2015/10

N2 - Background and Aims: A hallmark of inflammatory bowel disease [IBD] is chronic inflammation, which leads to excessive extracellular matrix [ECM] remodelling and release of specific protein fragments, called neoepitopes. We speculated that the biomarker profile panel for ulcerative colitis [UC] and Crohn's disease [CD] represent a heterogeneous expression pattern, and may be applied as a tool to aid in the differentiation between UC and CD.Methods: Serum biomarkers of degraded collagens I, III-IV [C1M, C3M, and C4M], collagen type 1 and IV formation [P1NP, P4NP], and citrullinated and MMP-degraded vimentin [VICM] were studied with a competitive ELISA assay system in a cohort including 164 subjects [CD n = 72, UC n = 60, and non-IBD controls n = 32] and a validation cohort of 61 subjects [CD n = 46, and UC n = 15]. Receiver operating characteristic curve analysis and logistic regression modelling were carried out to evaluate the discriminative power of the biomarkers.Results: All biomarkers were corrected for confounding factors. VICM and C3M demonstrated the highest diagnostic power, alone, to differentiate CD from UC with an area under the curve [AUC] of 0.77 and 0.69, respectively. Furthermore, the biomarkers C1M [AUC = 0.81], C3M [AUC = 0.83], VICM [AUC = 0.83], and P1NP [AUC = 0.77] were best to differentiate UC from non-IBD. The best combinations of biomarkers to differentiate CD from UC and UC from non-IBD were VICM, C3M, C4M [AUC = 0.90] and VICM, C3M [AUC = 0.98] respectively.Conclusions: Specific extracellular matrix degradation markers are elevated in IBD and can discriminate CD from UC and UC from non-IBD controls with a high diagnostic accuracy.

AB - Background and Aims: A hallmark of inflammatory bowel disease [IBD] is chronic inflammation, which leads to excessive extracellular matrix [ECM] remodelling and release of specific protein fragments, called neoepitopes. We speculated that the biomarker profile panel for ulcerative colitis [UC] and Crohn's disease [CD] represent a heterogeneous expression pattern, and may be applied as a tool to aid in the differentiation between UC and CD.Methods: Serum biomarkers of degraded collagens I, III-IV [C1M, C3M, and C4M], collagen type 1 and IV formation [P1NP, P4NP], and citrullinated and MMP-degraded vimentin [VICM] were studied with a competitive ELISA assay system in a cohort including 164 subjects [CD n = 72, UC n = 60, and non-IBD controls n = 32] and a validation cohort of 61 subjects [CD n = 46, and UC n = 15]. Receiver operating characteristic curve analysis and logistic regression modelling were carried out to evaluate the discriminative power of the biomarkers.Results: All biomarkers were corrected for confounding factors. VICM and C3M demonstrated the highest diagnostic power, alone, to differentiate CD from UC with an area under the curve [AUC] of 0.77 and 0.69, respectively. Furthermore, the biomarkers C1M [AUC = 0.81], C3M [AUC = 0.83], VICM [AUC = 0.83], and P1NP [AUC = 0.77] were best to differentiate UC from non-IBD. The best combinations of biomarkers to differentiate CD from UC and UC from non-IBD were VICM, C3M, C4M [AUC = 0.90] and VICM, C3M [AUC = 0.98] respectively.Conclusions: Specific extracellular matrix degradation markers are elevated in IBD and can discriminate CD from UC and UC from non-IBD controls with a high diagnostic accuracy.

KW - Inflammatory bowel disease

KW - serological biomarkers

KW - extracellular matrix remodelling

KW - INFLAMMATORY-BOWEL-DISEASE

KW - MATRIX METALLOPROTEINASES

KW - ANKYLOSING-SPONDYLITIS

KW - RHEUMATOID-ARTHRITIS

KW - CONNECTIVE-TISSUE

KW - EXPRESSION

KW - ANTIBODIES

KW - INHIBITORS

KW - TURNOVER

KW - MARKERS

U2 - 10.1093/ecco-jcc/jjv123

DO - 10.1093/ecco-jcc/jjv123

M3 - Article

VL - 9

SP - 863

EP - 872

JO - Journal of Crohn's and Colitis

JF - Journal of Crohn's and Colitis

SN - 1873-9946

IS - 10

ER -

ID: 22390577