Publication

Four key signaling pathways mediating chemotaxis in Dictyostelium discoideum

Veltman, D. M., Keizer-Gunnink, I. & Van Haastert, P. J. M., 25-Feb-2008, In : Journal of Cell Biology. 180, 4, p. 747-753 7 p.

Research output: Contribution to journalArticleAcademicpeer-review

APA

Veltman, D. M., Keizer-Gunnink, I., & Van Haastert, P. J. M. (2008). Four key signaling pathways mediating chemotaxis in Dictyostelium discoideum. Journal of Cell Biology, 180(4), 747-753. https://doi.org/10.1083/jcb.200709180

Author

Veltman, Douwe M. ; Keizer-Gunnink, Ineke ; Van Haastert, Peter J. M. / Four key signaling pathways mediating chemotaxis in Dictyostelium discoideum. In: Journal of Cell Biology. 2008 ; Vol. 180, No. 4. pp. 747-753.

Harvard

Veltman, DM, Keizer-Gunnink, I & Van Haastert, PJM 2008, 'Four key signaling pathways mediating chemotaxis in Dictyostelium discoideum', Journal of Cell Biology, vol. 180, no. 4, pp. 747-753. https://doi.org/10.1083/jcb.200709180

Standard

Four key signaling pathways mediating chemotaxis in Dictyostelium discoideum. / Veltman, Douwe M.; Keizer-Gunnink, Ineke; Van Haastert, Peter J. M.

In: Journal of Cell Biology, Vol. 180, No. 4, 25.02.2008, p. 747-753.

Research output: Contribution to journalArticleAcademicpeer-review

Vancouver

Veltman DM, Keizer-Gunnink I, Van Haastert PJM. Four key signaling pathways mediating chemotaxis in Dictyostelium discoideum. Journal of Cell Biology. 2008 Feb 25;180(4):747-753. https://doi.org/10.1083/jcb.200709180


BibTeX

@article{9922a31cd3f0418f9d173b4edfb0abd3,
title = "Four key signaling pathways mediating chemotaxis in Dictyostelium discoideum",
abstract = "Chemotaxis is the ability of cells to move in the direction of an external gradient of signaling molecules. Cells are guided by actin-filled protrusions in the front, whereas myosin filaments retract the rear of the cell. Previous work demonstrated that chernotaxis of unpolarized amoeboid Dictyostelium discoideum cells is mediated by two parallel pathways, phosphoinositide-3-kinase (PI3K) and phospholipase A2 (PLA2). Here, we show that polarized cells exhibit very good chernotaxis with inhibited PI3K and PLA2 activity. Using genetic screens, we demonstrate that this activity is mediated by a soluble guanylyl cyclase, providing two signals. The protein localizes to the leading edge where it interacts with actin filaments, whereas the cyclic guanosine monophosphate product induces myosin filaments in the rear of the cell. We conclude that chernotaxis is mediated by multiple signaling pathways regulating protrusions at the front and rear of the cell. Cells that express only rear activity are polarized but do not exhibit chemotaxis, whereas cells with only front signaling are unpolarized but undergo chemotaxis.",
keywords = "GUANYLYL CYCLASE, PHOSPHOLIPASE-C, CELL-MOVEMENT, LEADING-EDGE, PROTEIN, ROLES, INHIBITION, PI3-KINASE, CHEMOKINES, POLARITY",
author = "Veltman, {Douwe M.} and Ineke Keizer-Gunnink and {Van Haastert}, {Peter J. M.}",
year = "2008",
month = feb,
day = "25",
doi = "10.1083/jcb.200709180",
language = "English",
volume = "180",
pages = "747--753",
journal = "The Journal of Cell Biology",
issn = "0021-9525",
publisher = "ROCKEFELLER UNIV PRESS",
number = "4",

}

RIS

TY - JOUR

T1 - Four key signaling pathways mediating chemotaxis in Dictyostelium discoideum

AU - Veltman, Douwe M.

AU - Keizer-Gunnink, Ineke

AU - Van Haastert, Peter J. M.

PY - 2008/2/25

Y1 - 2008/2/25

N2 - Chemotaxis is the ability of cells to move in the direction of an external gradient of signaling molecules. Cells are guided by actin-filled protrusions in the front, whereas myosin filaments retract the rear of the cell. Previous work demonstrated that chernotaxis of unpolarized amoeboid Dictyostelium discoideum cells is mediated by two parallel pathways, phosphoinositide-3-kinase (PI3K) and phospholipase A2 (PLA2). Here, we show that polarized cells exhibit very good chernotaxis with inhibited PI3K and PLA2 activity. Using genetic screens, we demonstrate that this activity is mediated by a soluble guanylyl cyclase, providing two signals. The protein localizes to the leading edge where it interacts with actin filaments, whereas the cyclic guanosine monophosphate product induces myosin filaments in the rear of the cell. We conclude that chernotaxis is mediated by multiple signaling pathways regulating protrusions at the front and rear of the cell. Cells that express only rear activity are polarized but do not exhibit chemotaxis, whereas cells with only front signaling are unpolarized but undergo chemotaxis.

AB - Chemotaxis is the ability of cells to move in the direction of an external gradient of signaling molecules. Cells are guided by actin-filled protrusions in the front, whereas myosin filaments retract the rear of the cell. Previous work demonstrated that chernotaxis of unpolarized amoeboid Dictyostelium discoideum cells is mediated by two parallel pathways, phosphoinositide-3-kinase (PI3K) and phospholipase A2 (PLA2). Here, we show that polarized cells exhibit very good chernotaxis with inhibited PI3K and PLA2 activity. Using genetic screens, we demonstrate that this activity is mediated by a soluble guanylyl cyclase, providing two signals. The protein localizes to the leading edge where it interacts with actin filaments, whereas the cyclic guanosine monophosphate product induces myosin filaments in the rear of the cell. We conclude that chernotaxis is mediated by multiple signaling pathways regulating protrusions at the front and rear of the cell. Cells that express only rear activity are polarized but do not exhibit chemotaxis, whereas cells with only front signaling are unpolarized but undergo chemotaxis.

KW - GUANYLYL CYCLASE

KW - PHOSPHOLIPASE-C

KW - CELL-MOVEMENT

KW - LEADING-EDGE

KW - PROTEIN

KW - ROLES

KW - INHIBITION

KW - PI3-KINASE

KW - CHEMOKINES

KW - POLARITY

U2 - 10.1083/jcb.200709180

DO - 10.1083/jcb.200709180

M3 - Article

VL - 180

SP - 747

EP - 753

JO - The Journal of Cell Biology

JF - The Journal of Cell Biology

SN - 0021-9525

IS - 4

ER -

ID: 4687153