Four key signaling pathways mediating chemotaxis in Dictyostelium discoideumVeltman, D. M., Keizer-Gunnink, I. & Van Haastert, P. J. M., 25-Feb-2008, In : Journal of Cell Biology. 180, 4, p. 747-753 7 p.
Research output: Contribution to journal › Article › Academic › peer-review
Chemotaxis is the ability of cells to move in the direction of an external gradient of signaling molecules. Cells are guided by actin-filled protrusions in the front, whereas myosin filaments retract the rear of the cell. Previous work demonstrated that chernotaxis of unpolarized amoeboid Dictyostelium discoideum cells is mediated by two parallel pathways, phosphoinositide-3-kinase (PI3K) and phospholipase A2 (PLA2). Here, we show that polarized cells exhibit very good chernotaxis with inhibited PI3K and PLA2 activity. Using genetic screens, we demonstrate that this activity is mediated by a soluble guanylyl cyclase, providing two signals. The protein localizes to the leading edge where it interacts with actin filaments, whereas the cyclic guanosine monophosphate product induces myosin filaments in the rear of the cell. We conclude that chernotaxis is mediated by multiple signaling pathways regulating protrusions at the front and rear of the cell. Cells that express only rear activity are polarized but do not exhibit chemotaxis, whereas cells with only front signaling are unpolarized but undergo chemotaxis.
|Number of pages||7|
|Journal||Journal of Cell Biology|
|Publication status||Published - 25-Feb-2008|
- GUANYLYL CYCLASE, PHOSPHOLIPASE-C, CELL-MOVEMENT, LEADING-EDGE, PROTEIN, ROLES, INHIBITION, PI3-KINASE, CHEMOKINES, POLARITY