Publication

Four key signaling pathways mediating chemotaxis in Dictyostelium discoideum

Veltman, D. M., Keizer-Gunnink, I. & Van Haastert, P. J. M., 25-Feb-2008, In : Journal of Cell Biology. 180, 4, p. 747-753 7 p.

Research output: Contribution to journalArticleAcademicpeer-review

Chemotaxis is the ability of cells to move in the direction of an external gradient of signaling molecules. Cells are guided by actin-filled protrusions in the front, whereas myosin filaments retract the rear of the cell. Previous work demonstrated that chernotaxis of unpolarized amoeboid Dictyostelium discoideum cells is mediated by two parallel pathways, phosphoinositide-3-kinase (PI3K) and phospholipase A2 (PLA2). Here, we show that polarized cells exhibit very good chernotaxis with inhibited PI3K and PLA2 activity. Using genetic screens, we demonstrate that this activity is mediated by a soluble guanylyl cyclase, providing two signals. The protein localizes to the leading edge where it interacts with actin filaments, whereas the cyclic guanosine monophosphate product induces myosin filaments in the rear of the cell. We conclude that chernotaxis is mediated by multiple signaling pathways regulating protrusions at the front and rear of the cell. Cells that express only rear activity are polarized but do not exhibit chemotaxis, whereas cells with only front signaling are unpolarized but undergo chemotaxis.

Original languageEnglish
Pages (from-to)747-753
Number of pages7
JournalJournal of Cell Biology
Volume180
Issue number4
Publication statusPublished - 25-Feb-2008

    Keywords

  • GUANYLYL CYCLASE, PHOSPHOLIPASE-C, CELL-MOVEMENT, LEADING-EDGE, PROTEIN, ROLES, INHIBITION, PI3-KINASE, CHEMOKINES, POLARITY

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