FOSL1 Controls the Assembly of Endothelial Cells into Capillary Tubes by Direct Repression of ﰀv and ﰁ3 Integrin TranscriptionEvellin, S., Galvagni, F., Zippo, A., Neri, F., Orlandini, M., Incarnato, D., Dettori, D., Neubauer, S., Kessler, H., Wagner, E. F. & Oliviero, S., Mar-2013, In : Molecular and Cellular Biology. 33, 6, p. 1198-1209 12 p.
Research output: Contribution to journal › Article › Academic › peer-review
To form three-dimensional capillary tubes, endothelial cells must establish contacts with the extracellular matrix that provides signals for their proliferation, migration, and differentiation. The transcription factor Fosl1 plays a key role in the vasculogenic and angiogenic processes as Fosl1 knockout embryos die with vascular defects in extraembryonic tissues. Here, we show that Fosl1ﰋ/ﰋ embryonic stem cells differentiate into endothelial cells but fail to correctly assemble into primitive capillaries and to form tube-like structures. FOSL1 silencing affects in vitro angiogenesis, increases cell adhesion, and decreases cell mobility of primary human endothelial cells (HUVEC). We further show that FOSL1 is a repressor of ﰀv and ﰁ3 integrin expression and that the down-modulation of ﰀvﰁ3 rescues the angiogenic phenotype in FOSL1-silenced HUVEC, while the ectopic expression of ﰀvﰁ3 alone reproduces the phenotypic alterations induced by FOSL1 knockdown. FOSL1 represses the transcription of both ﰀv and ﰁ3 integrin genes by binding together with JunD to their proximal promoter via the transcription factor SP1. These data suggest that FOSL1-dependent negative regulation of ﰀvﰁ3 expression on endothelial cells is required for endothelial assembly into vessel structures.
|Number of pages||12|
|Journal||Molecular and Cellular Biology|
|Publication status||Published - Mar-2013|