Publication

Formation of the ether lipids archaetidylglycerol and archaetidylethanolamine in Escherichia coli

Caforio, A., Jain, S., Fodran, P., Siliakus, M., Minnaard, A. J., van der Oost, J. & Driessen, A. J. M., 20-Jul-2015, In : Biochemical Journal. 470, 3, p. 343-355 13 p., 20150626.

Research output: Contribution to journalArticleAcademicpeer-review

APA

Caforio, A., Jain, S., Fodran, P., Siliakus, M., Minnaard, A. J., van der Oost, J., & Driessen, A. J. M. (2015). Formation of the ether lipids archaetidylglycerol and archaetidylethanolamine in Escherichia coli. Biochemical Journal, 470(3), 343-355. [20150626]. https://doi.org/10.1042/BJ20150626

Author

Caforio, Antonella ; Jain, Samta ; Fodran, Peter ; Siliakus, Melvin ; Minnaard, Adriaan J ; van der Oost, John ; Driessen, Arnold J M. / Formation of the ether lipids archaetidylglycerol and archaetidylethanolamine in Escherichia coli. In: Biochemical Journal. 2015 ; Vol. 470, No. 3. pp. 343-355.

Harvard

Caforio, A, Jain, S, Fodran, P, Siliakus, M, Minnaard, AJ, van der Oost, J & Driessen, AJM 2015, 'Formation of the ether lipids archaetidylglycerol and archaetidylethanolamine in Escherichia coli', Biochemical Journal, vol. 470, no. 3, 20150626, pp. 343-355. https://doi.org/10.1042/BJ20150626

Standard

Formation of the ether lipids archaetidylglycerol and archaetidylethanolamine in Escherichia coli. / Caforio, Antonella; Jain, Samta; Fodran, Peter; Siliakus, Melvin; Minnaard, Adriaan J; van der Oost, John; Driessen, Arnold J M.

In: Biochemical Journal, Vol. 470, No. 3, 20150626, 20.07.2015, p. 343-355.

Research output: Contribution to journalArticleAcademicpeer-review

Vancouver

Caforio A, Jain S, Fodran P, Siliakus M, Minnaard AJ, van der Oost J et al. Formation of the ether lipids archaetidylglycerol and archaetidylethanolamine in Escherichia coli. Biochemical Journal. 2015 Jul 20;470(3):343-355. 20150626. https://doi.org/10.1042/BJ20150626


BibTeX

@article{05e80b6ada5d440badee996a6e492a42,
title = "Formation of the ether lipids archaetidylglycerol and archaetidylethanolamine in Escherichia coli",
abstract = "In archaea, the membrane phospholipids consist of isoprenoid hydrocarbon chains that are ether-linked to a sn -glycerol-1-phosphate backbone. This unique structure is believed to be vital for the adaptation of these microorganisms to extreme environments, but it also reflects an evolutionary marker that distinguishes archaea from bacteria and eukaryotes. CDP-archaeol is the central precursor for polar head group attachment. We examined various bacterial enzymes involved in the attachment of L-serine and glycerol as polar head groups for their promiscuity in recognizing CDP-archaeol as a substrate. Using a combination of mutated bacterial and archaeal enzymes, archaetidylethanolamine and archaetidylglycerol could be produced in vitro using nine purified enzymes while starting from simple building blocks. The ether lipid pathway constituted by a set of archaeal and bacterial enzymes was introduced into E. coli , which resulted in the biosynthesis of archaetidylethanolamine and archaetidylglycerol. This is a further step in the reprogramming of E. coli for ether lipid biosynthesis.",
author = "Antonella Caforio and Samta Jain and Peter Fodran and Melvin Siliakus and Minnaard, {Adriaan J} and {van der Oost}, John and Driessen, {Arnold J M}",
note = "Copyright 2015 The Author(s).",
year = "2015",
month = "7",
day = "20",
doi = "10.1042/BJ20150626",
language = "English",
volume = "470",
pages = "343--355",
journal = "Biochemical Journal",
issn = "0264-6021",
publisher = "PORTLAND PRESS LTD",
number = "3",

}

RIS

TY - JOUR

T1 - Formation of the ether lipids archaetidylglycerol and archaetidylethanolamine in Escherichia coli

AU - Caforio, Antonella

AU - Jain, Samta

AU - Fodran, Peter

AU - Siliakus, Melvin

AU - Minnaard, Adriaan J

AU - van der Oost, John

AU - Driessen, Arnold J M

N1 - Copyright 2015 The Author(s).

PY - 2015/7/20

Y1 - 2015/7/20

N2 - In archaea, the membrane phospholipids consist of isoprenoid hydrocarbon chains that are ether-linked to a sn -glycerol-1-phosphate backbone. This unique structure is believed to be vital for the adaptation of these microorganisms to extreme environments, but it also reflects an evolutionary marker that distinguishes archaea from bacteria and eukaryotes. CDP-archaeol is the central precursor for polar head group attachment. We examined various bacterial enzymes involved in the attachment of L-serine and glycerol as polar head groups for their promiscuity in recognizing CDP-archaeol as a substrate. Using a combination of mutated bacterial and archaeal enzymes, archaetidylethanolamine and archaetidylglycerol could be produced in vitro using nine purified enzymes while starting from simple building blocks. The ether lipid pathway constituted by a set of archaeal and bacterial enzymes was introduced into E. coli , which resulted in the biosynthesis of archaetidylethanolamine and archaetidylglycerol. This is a further step in the reprogramming of E. coli for ether lipid biosynthesis.

AB - In archaea, the membrane phospholipids consist of isoprenoid hydrocarbon chains that are ether-linked to a sn -glycerol-1-phosphate backbone. This unique structure is believed to be vital for the adaptation of these microorganisms to extreme environments, but it also reflects an evolutionary marker that distinguishes archaea from bacteria and eukaryotes. CDP-archaeol is the central precursor for polar head group attachment. We examined various bacterial enzymes involved in the attachment of L-serine and glycerol as polar head groups for their promiscuity in recognizing CDP-archaeol as a substrate. Using a combination of mutated bacterial and archaeal enzymes, archaetidylethanolamine and archaetidylglycerol could be produced in vitro using nine purified enzymes while starting from simple building blocks. The ether lipid pathway constituted by a set of archaeal and bacterial enzymes was introduced into E. coli , which resulted in the biosynthesis of archaetidylethanolamine and archaetidylglycerol. This is a further step in the reprogramming of E. coli for ether lipid biosynthesis.

U2 - 10.1042/BJ20150626

DO - 10.1042/BJ20150626

M3 - Article

VL - 470

SP - 343

EP - 355

JO - Biochemical Journal

JF - Biochemical Journal

SN - 0264-6021

IS - 3

M1 - 20150626

ER -

ID: 23055396