Formation of the ether lipids archaetidylglycerol and archaetidylethanolamine in Escherichia coliCaforio, A., Jain, S., Fodran, P., Siliakus, M., Minnaard, A. J., van der Oost, J. & Driessen, A. J. M., 20-Jul-2015, In : Biochemical Journal. 470, 3, p. 343-355 13 p., 20150626.
Research output: Contribution to journal › Article › Academic › peer-review
In archaea, the membrane phospholipids consist of isoprenoid hydrocarbon chains that are ether-linked to a sn -glycerol-1-phosphate backbone. This unique structure is believed to be vital for the adaptation of these microorganisms to extreme environments, but it also reflects an evolutionary marker that distinguishes archaea from bacteria and eukaryotes. CDP-archaeol is the central precursor for polar head group attachment. We examined various bacterial enzymes involved in the attachment of L-serine and glycerol as polar head groups for their promiscuity in recognizing CDP-archaeol as a substrate. Using a combination of mutated bacterial and archaeal enzymes, archaetidylethanolamine and archaetidylglycerol could be produced in vitro using nine purified enzymes while starting from simple building blocks. The ether lipid pathway constituted by a set of archaeal and bacterial enzymes was introduced into E. coli , which resulted in the biosynthesis of archaetidylethanolamine and archaetidylglycerol. This is a further step in the reprogramming of E. coli for ether lipid biosynthesis.
|Number of pages||13|
|Publication status||Published - 20-Jul-2015|