Publication

Formation and electrical characterization of black lipid membranes in porous filter materials

Aminipour, Z., Khorshid, M., Bayoumi, M., Losada-Perez, P., Thoelen, R., Bonakdar, S., Keshvari, H., Maglia, G., Wagner, P. & Van der Bruggen, B., Sep-2017, In : Physica Status Solidi A-Applications and materials science. 214, 9, 8 p., 1700104.

Research output: Contribution to journalArticleAcademicpeer-review

APA

Aminipour, Z., Khorshid, M., Bayoumi, M., Losada-Perez, P., Thoelen, R., Bonakdar, S., ... Van der Bruggen, B. (2017). Formation and electrical characterization of black lipid membranes in porous filter materials. Physica Status Solidi A-Applications and materials science, 214(9), [1700104]. https://doi.org/10.1002/pssa.201700104

Author

Aminipour, Zahra ; Khorshid, Mehran ; Bayoumi, Mariam ; Losada-Perez, Patricia ; Thoelen, Ronald ; Bonakdar, Shahin ; Keshvari, Hamid ; Maglia, Giovanni ; Wagner, Patrick ; Van der Bruggen, Bart. / Formation and electrical characterization of black lipid membranes in porous filter materials. In: Physica Status Solidi A-Applications and materials science. 2017 ; Vol. 214, No. 9.

Harvard

Aminipour, Z, Khorshid, M, Bayoumi, M, Losada-Perez, P, Thoelen, R, Bonakdar, S, Keshvari, H, Maglia, G, Wagner, P & Van der Bruggen, B 2017, 'Formation and electrical characterization of black lipid membranes in porous filter materials', Physica Status Solidi A-Applications and materials science, vol. 214, no. 9, 1700104. https://doi.org/10.1002/pssa.201700104

Standard

Formation and electrical characterization of black lipid membranes in porous filter materials. / Aminipour, Zahra; Khorshid, Mehran; Bayoumi, Mariam; Losada-Perez, Patricia; Thoelen, Ronald; Bonakdar, Shahin; Keshvari, Hamid; Maglia, Giovanni; Wagner, Patrick; Van der Bruggen, Bart.

In: Physica Status Solidi A-Applications and materials science, Vol. 214, No. 9, 1700104, 09.2017.

Research output: Contribution to journalArticleAcademicpeer-review

Vancouver

Aminipour Z, Khorshid M, Bayoumi M, Losada-Perez P, Thoelen R, Bonakdar S et al. Formation and electrical characterization of black lipid membranes in porous filter materials. Physica Status Solidi A-Applications and materials science. 2017 Sep;214(9). 1700104. https://doi.org/10.1002/pssa.201700104


BibTeX

@article{a516f4efb34049e68e17d332ad991506,
title = "Formation and electrical characterization of black lipid membranes in porous filter materials",
abstract = "Black lipid membranes (BLMs) are planar lipid bilayers that can be formed in porous supports such as filters. A BLM as a biomimetic membrane has important applications in, for instance, biosensing and drug-permeability assays. In the present study, BLMs were formed in the pores of three different filters to investigate their ability to support the formation of a high-quality lipid bilayer. Poly (vinylidene fluoride) (PVDF), poly(acrylonitrile) (PAN), and polypropylene/polyethylene (PP/PE) filters were utilized as filter supports, and the bilayer formation was monitored in situ by capacitance measurements. The maximum capacitance was obtained for PVDF (5.34Fcm(-2)) versus PP/PE and PAN (2.95 and 0.91Fcm(-2), respectively). Additionally, BLM leakage current measurements by wave deviation provided consistent results. Thickness and compactness of the bilayers were evaluated using pore forming protein (ClyA) insertion. The results show that PVDF with the highest porosity level was the most suitable support for BLM formation. The optimal thickness for the BLM in PVDF was 3-5nm, where the most efficient protein insertion took place, in comparison with other filters.",
keywords = "bilayers, biosensors, black lipid membranes, filters, porous materials, protein nanopores",
author = "Zahra Aminipour and Mehran Khorshid and Mariam Bayoumi and Patricia Losada-Perez and Ronald Thoelen and Shahin Bonakdar and Hamid Keshvari and Giovanni Maglia and Patrick Wagner and {Van der Bruggen}, Bart",
year = "2017",
month = "9",
doi = "10.1002/pssa.201700104",
language = "English",
volume = "214",
journal = "Physica Status Solidi A-Applications and materials science",
issn = "1862-6300",
publisher = "WILEY-V C H VERLAG GMBH",
number = "9",

}

RIS

TY - JOUR

T1 - Formation and electrical characterization of black lipid membranes in porous filter materials

AU - Aminipour, Zahra

AU - Khorshid, Mehran

AU - Bayoumi, Mariam

AU - Losada-Perez, Patricia

AU - Thoelen, Ronald

AU - Bonakdar, Shahin

AU - Keshvari, Hamid

AU - Maglia, Giovanni

AU - Wagner, Patrick

AU - Van der Bruggen, Bart

PY - 2017/9

Y1 - 2017/9

N2 - Black lipid membranes (BLMs) are planar lipid bilayers that can be formed in porous supports such as filters. A BLM as a biomimetic membrane has important applications in, for instance, biosensing and drug-permeability assays. In the present study, BLMs were formed in the pores of three different filters to investigate their ability to support the formation of a high-quality lipid bilayer. Poly (vinylidene fluoride) (PVDF), poly(acrylonitrile) (PAN), and polypropylene/polyethylene (PP/PE) filters were utilized as filter supports, and the bilayer formation was monitored in situ by capacitance measurements. The maximum capacitance was obtained for PVDF (5.34Fcm(-2)) versus PP/PE and PAN (2.95 and 0.91Fcm(-2), respectively). Additionally, BLM leakage current measurements by wave deviation provided consistent results. Thickness and compactness of the bilayers were evaluated using pore forming protein (ClyA) insertion. The results show that PVDF with the highest porosity level was the most suitable support for BLM formation. The optimal thickness for the BLM in PVDF was 3-5nm, where the most efficient protein insertion took place, in comparison with other filters.

AB - Black lipid membranes (BLMs) are planar lipid bilayers that can be formed in porous supports such as filters. A BLM as a biomimetic membrane has important applications in, for instance, biosensing and drug-permeability assays. In the present study, BLMs were formed in the pores of three different filters to investigate their ability to support the formation of a high-quality lipid bilayer. Poly (vinylidene fluoride) (PVDF), poly(acrylonitrile) (PAN), and polypropylene/polyethylene (PP/PE) filters were utilized as filter supports, and the bilayer formation was monitored in situ by capacitance measurements. The maximum capacitance was obtained for PVDF (5.34Fcm(-2)) versus PP/PE and PAN (2.95 and 0.91Fcm(-2), respectively). Additionally, BLM leakage current measurements by wave deviation provided consistent results. Thickness and compactness of the bilayers were evaluated using pore forming protein (ClyA) insertion. The results show that PVDF with the highest porosity level was the most suitable support for BLM formation. The optimal thickness for the BLM in PVDF was 3-5nm, where the most efficient protein insertion took place, in comparison with other filters.

KW - bilayers

KW - biosensors

KW - black lipid membranes

KW - filters

KW - porous materials

KW - protein nanopores

U2 - 10.1002/pssa.201700104

DO - 10.1002/pssa.201700104

M3 - Article

VL - 214

JO - Physica Status Solidi A-Applications and materials science

JF - Physica Status Solidi A-Applications and materials science

SN - 1862-6300

IS - 9

M1 - 1700104

ER -

ID: 48387632