Publication

Flunarizine and r-56865 suppress veratridine-induced increase in oxygen-consumption and uptake of ca-45(2+) in rat cortical synaptosomes

WERMELSKIRCHEN, D., GLEITZ, J., URENJAK, J., WILFFERT, B., TEGTMEIER, F. & Peters, T., Mar-1992, In : Neuropharmacology. 31, 3, p. 235-241 7 p.

Research output: Contribution to journalArticleAcademicpeer-review

APA

WERMELSKIRCHEN, D., GLEITZ, J., URENJAK, J., WILFFERT, B., TEGTMEIER, F., & Peters, T. (1992). Flunarizine and r-56865 suppress veratridine-induced increase in oxygen-consumption and uptake of ca-45(2+) in rat cortical synaptosomes. Neuropharmacology, 31(3), 235-241.

Author

WERMELSKIRCHEN, D ; GLEITZ, J ; URENJAK, J ; WILFFERT, B ; TEGTMEIER, F ; Peters, Thies. / Flunarizine and r-56865 suppress veratridine-induced increase in oxygen-consumption and uptake of ca-45(2+) in rat cortical synaptosomes. In: Neuropharmacology. 1992 ; Vol. 31, No. 3. pp. 235-241.

Harvard

WERMELSKIRCHEN, D, GLEITZ, J, URENJAK, J, WILFFERT, B, TEGTMEIER, F & Peters, T 1992, 'Flunarizine and r-56865 suppress veratridine-induced increase in oxygen-consumption and uptake of ca-45(2+) in rat cortical synaptosomes', Neuropharmacology, vol. 31, no. 3, pp. 235-241.

Standard

Flunarizine and r-56865 suppress veratridine-induced increase in oxygen-consumption and uptake of ca-45(2+) in rat cortical synaptosomes. / WERMELSKIRCHEN, D; GLEITZ, J; URENJAK, J; WILFFERT, B; TEGTMEIER, F; Peters, Thies.

In: Neuropharmacology, Vol. 31, No. 3, 03.1992, p. 235-241.

Research output: Contribution to journalArticleAcademicpeer-review

Vancouver

WERMELSKIRCHEN D, GLEITZ J, URENJAK J, WILFFERT B, TEGTMEIER F, Peters T. Flunarizine and r-56865 suppress veratridine-induced increase in oxygen-consumption and uptake of ca-45(2+) in rat cortical synaptosomes. Neuropharmacology. 1992 Mar;31(3):235-241.


BibTeX

@article{21c940dc305b474294610380f43c4e95,
title = "Flunarizine and r-56865 suppress veratridine-induced increase in oxygen-consumption and uptake of ca-45(2+) in rat cortical synaptosomes",
abstract = "The effect of the anti-ischemic compounds flunarizine and R 56865 on the veratridine-induced uptake of Ca2+ and Na+ was observed in cortical synaptosomes in the rat. The veratridine-induced uptake of Na+ and Ca2+ was determined by means of a measurement of synaptosomal oxygen consumption and a method for the uptake of Ca-45(2+), respectively. Veratridine (10(-5) M) was found to induce a 3-fold increase in synaptosomal oxygen consumption (uptake of Na+) and uptake of Ca-45(2+), both of which were inhibited by tetrodotoxin (10(-5) M). Nitrendipine (10(-5) M) and omega-conotoxin (5 x 10(-7) M) were ineffective on the veratridine-induced response. Nimodipine (10(-5) M) suppressed the veratridine-induced uptake of Ca-45(2+) but also diminished the unstimulated uptake of Ca-45(2+). The veratridine-induced uptake of Na+ was not influenced by nimodipine. Flunarizine (3 x 10(-6)-10(-5) M), as well as R 56865 (10(-6)-10(-5) M), attenuated the veratridine-induced uptake of both Na+ and Ca-45(2+). In conclusion, the veratridine-induced uptake of Na+ and Ca-45(2+) was shown to be closely correlated to the activity of Na+ channels but not to voltage-operated Ca2+ channels. Secondly, flunarizine and R 56865 seemed to evoke their effects by interfering with the permeability of Na+ channels. Since veratridine-induced uptake of Na+ and Ca2+ shares some similarities with ischaemia-induced uptake of Na+ and Ca2+, it is proposed, that flunarizine and R 56865 exert their anti-ischaemic effects by reducing ischaemia-induced Na+ and Ca2+ load, probably by inhibiting a TTX-sensitive Na+ channel.",
keywords = "VERATRIDINE, OXYGEN CONSUMPTION, CA-45(2+) UPTAKE, NA+ CHANNEL, CALCIUM CHANNELS, SODIUM INFLUX, NA CHANNELS, BRAIN, ENTRY, TETRODOTOXIN, BINDING, DAMAGE, ANTAGONIST, PRINCIPLE",
author = "D WERMELSKIRCHEN and J GLEITZ and J URENJAK and B WILFFERT and F TEGTMEIER and Thies Peters",
year = "1992",
month = "3",
language = "English",
volume = "31",
pages = "235--241",
journal = "Neuropharmacology",
issn = "0028-3908",
publisher = "PERGAMON-ELSEVIER SCIENCE LTD",
number = "3",

}

RIS

TY - JOUR

T1 - Flunarizine and r-56865 suppress veratridine-induced increase in oxygen-consumption and uptake of ca-45(2+) in rat cortical synaptosomes

AU - WERMELSKIRCHEN, D

AU - GLEITZ, J

AU - URENJAK, J

AU - WILFFERT, B

AU - TEGTMEIER, F

AU - Peters, Thies

PY - 1992/3

Y1 - 1992/3

N2 - The effect of the anti-ischemic compounds flunarizine and R 56865 on the veratridine-induced uptake of Ca2+ and Na+ was observed in cortical synaptosomes in the rat. The veratridine-induced uptake of Na+ and Ca2+ was determined by means of a measurement of synaptosomal oxygen consumption and a method for the uptake of Ca-45(2+), respectively. Veratridine (10(-5) M) was found to induce a 3-fold increase in synaptosomal oxygen consumption (uptake of Na+) and uptake of Ca-45(2+), both of which were inhibited by tetrodotoxin (10(-5) M). Nitrendipine (10(-5) M) and omega-conotoxin (5 x 10(-7) M) were ineffective on the veratridine-induced response. Nimodipine (10(-5) M) suppressed the veratridine-induced uptake of Ca-45(2+) but also diminished the unstimulated uptake of Ca-45(2+). The veratridine-induced uptake of Na+ was not influenced by nimodipine. Flunarizine (3 x 10(-6)-10(-5) M), as well as R 56865 (10(-6)-10(-5) M), attenuated the veratridine-induced uptake of both Na+ and Ca-45(2+). In conclusion, the veratridine-induced uptake of Na+ and Ca-45(2+) was shown to be closely correlated to the activity of Na+ channels but not to voltage-operated Ca2+ channels. Secondly, flunarizine and R 56865 seemed to evoke their effects by interfering with the permeability of Na+ channels. Since veratridine-induced uptake of Na+ and Ca2+ shares some similarities with ischaemia-induced uptake of Na+ and Ca2+, it is proposed, that flunarizine and R 56865 exert their anti-ischaemic effects by reducing ischaemia-induced Na+ and Ca2+ load, probably by inhibiting a TTX-sensitive Na+ channel.

AB - The effect of the anti-ischemic compounds flunarizine and R 56865 on the veratridine-induced uptake of Ca2+ and Na+ was observed in cortical synaptosomes in the rat. The veratridine-induced uptake of Na+ and Ca2+ was determined by means of a measurement of synaptosomal oxygen consumption and a method for the uptake of Ca-45(2+), respectively. Veratridine (10(-5) M) was found to induce a 3-fold increase in synaptosomal oxygen consumption (uptake of Na+) and uptake of Ca-45(2+), both of which were inhibited by tetrodotoxin (10(-5) M). Nitrendipine (10(-5) M) and omega-conotoxin (5 x 10(-7) M) were ineffective on the veratridine-induced response. Nimodipine (10(-5) M) suppressed the veratridine-induced uptake of Ca-45(2+) but also diminished the unstimulated uptake of Ca-45(2+). The veratridine-induced uptake of Na+ was not influenced by nimodipine. Flunarizine (3 x 10(-6)-10(-5) M), as well as R 56865 (10(-6)-10(-5) M), attenuated the veratridine-induced uptake of both Na+ and Ca-45(2+). In conclusion, the veratridine-induced uptake of Na+ and Ca-45(2+) was shown to be closely correlated to the activity of Na+ channels but not to voltage-operated Ca2+ channels. Secondly, flunarizine and R 56865 seemed to evoke their effects by interfering with the permeability of Na+ channels. Since veratridine-induced uptake of Na+ and Ca2+ shares some similarities with ischaemia-induced uptake of Na+ and Ca2+, it is proposed, that flunarizine and R 56865 exert their anti-ischaemic effects by reducing ischaemia-induced Na+ and Ca2+ load, probably by inhibiting a TTX-sensitive Na+ channel.

KW - VERATRIDINE

KW - OXYGEN CONSUMPTION

KW - CA-45(2+) UPTAKE

KW - NA+ CHANNEL

KW - CALCIUM CHANNELS

KW - SODIUM INFLUX

KW - NA CHANNELS

KW - BRAIN

KW - ENTRY

KW - TETRODOTOXIN

KW - BINDING

KW - DAMAGE

KW - ANTAGONIST

KW - PRINCIPLE

M3 - Article

VL - 31

SP - 235

EP - 241

JO - Neuropharmacology

JF - Neuropharmacology

SN - 0028-3908

IS - 3

ER -

ID: 13859710