Filter Retardation Assay for Detecting and Quantifying Polyglutamine Aggregates Using Lysates

Sin, O., Mata-Cabana, A., Seinstra, R. I. & Nollen, E. A. A., 5-Oct-2018, In : JMIR research protocols. 8, 19, 9 p., 3042.

Research output: Contribution to journalArticleAcademicpeer-review

Protein aggregation is a hallmark of several neurodegenerative diseases and is associated with impaired protein homeostasis. This imbalance is caused by the loss of the protein's native conformation, which ultimately results in its aggregation or abnormal localization within the cell. Using a C. elegans model of polyglutamine diseases, we describe in detail the filter retardation assay, a method that captures protein aggregates in a cellulose acetate membrane and allows its detection and quantification by immunoblotting.

Original languageEnglish
Article number3042
Number of pages9
JournalJMIR research protocols
Issue number19
Publication statusPublished - 5-Oct-2018

ID: 67725504