Publication

Fibronectin attenuates process outgrowth in oligodendrocytes by mislocalizing MMP-9 activity

Siskova, Z., Yong, V. W., Nomden, A., van Strien, M., Hoekstra, D. & Baron, W., Oct-2009, In : Molecular and Cellular Neuroscience. 42, 3, p. 234-242 9 p.

Research output: Contribution to journalArticleAcademicpeer-review

The extension of multiple oligodendroglial branched processes towards axons is an important event during the early stages of myelination that likely requires remodeling of the extracellular matrix (ECM) micro-environment via matrix metalloproteinases (MMPs). Here we investigated whether fibronectin-mediated inhibition of myelin sheet formation in oligodendrocytes correlated with an altered MMP activity. Our data reveal that fibronectin enhanced, in a PKC-dependent manner, the net activity of MMP-9, but not its expression, in conditioned medium of oligodendrocytes. Residual cellular MMP-9 activity on fibronectin was confined to the cell body, whereas MMP-9 activity on laminin-2 was localized along extending processes of oligodendrocytes. The mislocalization of MMP-9 activity on fibronectin correlated with a perturbed outgrowth of oligodendroglial processes. In conclusion, our findings suggest that ECM molecules influence both the net activity of secreted MMP and the spatial distribution of cell-associated MMP activity, and thereby morphological oligodendrocyte differentiation. (C) 2009 Elsevier Inc. All rights reserved.

Original languageEnglish
Pages (from-to)234-242
Number of pages9
JournalMolecular and Cellular Neuroscience
Volume42
Issue number3
Publication statusPublished - Oct-2009

    Keywords

  • ECM, MMP-9, Myelin biogenesis, Oligodendrocytes, Process budding, MATRIX METALLOPROTEINASES, MULTIPLE-SCLEROSIS, TISSUE INHIBITOR, CELL-SURFACE, MORPHOLOGICAL-DIFFERENTIATION, VESICULAR TRAFFICKING, EXTRACELLULAR-MATRIX, CANCER PROGRESSION, NERVOUS-SYSTEM, EXPRESSION

ID: 5011610